Snap-freezing of brain tissue reveals its true structure-showing that cells are less squashed together, and the connections between neurons are less enclosed than previously thought.

Cryo preservation of brain tissue reveals dendritic spines with thinner necks compared to those prepared with chemical fixation.

Automated segmentation of neurons and identification of synapses in electron micrographs is significantly improved by using simple modifications to chemical fixation protocols that preserve extracellular space in the brain.

A broadly applicable method that faithfully preserves genetically labeled cellular structures for 3D electron microscopy (EM) and correlated light and electron microscopy (CLEM).

In situ X-ray imaging of biological tissue samples in staining solution while they are being processed for electron microscopy reveals novel insights into the diffusion-reaction kinetics, tissue mechanics, and staining quality.

Large-volume light microscopy combined with higher-resolution electron tomography revealed the spatial distribution of virus-producing cells and highlighted mechanisms of HIV-1 dissemination in bone marrow from a small animal model.

Eye movements time-locked to retrieval and novelty during learning preferentially occur at distinct phases of hippocampal theta.

Pericytes surrounding capillaries in the retina contain α-smooth muscle actin, demonstrating that pericytes have the necessary molecular machinery to change capillary diameter during neurovascular coupling.

While monitoring neuronal activity in freely moving Drosophila is challenging, HI-FISH enables brain-wide mapping of active neurons and reveals behavior-specific neural activity.

The ability to rapidly stain for any combination of genes in intact tissue with automated quantification of transcripts in individual cells and spatial re-mapping affords new insights into lung biology, and will greatly accelerate progress in scientific and medical research.