The absolute affinities of thousands of variant antibodies are measured in parallel using a combination of cell sorting and high-throughput DNA sequencing.

Simple guidelines and a checklist are provided for performing high-quality equilibrium binding measurements.

SSNA1 is a potent microtubule stabilizer and novel sensor of microtubule damage, acting on both microtubule ends and lattices to regulate microtubule dynamics and protect microtubules against severing activity.

The structural and mechanistic characterization of competitive inhibition of human DMT1 offers a promising route for the development of compounds for the treatment of iron overload disorders.

The anti-HIV drug lenacapavir breaks the HIV capsid by stabilising regions of low curvature at the expense of maintaining a closed shell, leading to loss of viral DNA synthesis and inhibition of infection.

Allosteric reorganizations of a bacterial channel receptor are mapped along the protein structure, using the site-directed bimane quenching fluorescence technique, through parallel real-time conformational and electrophysiological recordings.

Identification of a novel regulator that directly influences the assembly and function of the cell division machinery in industrially and medically important bacteria.

Absolute quantification of CTCF and cohesin reveals quantitative constraints on 3D genome organization and illuminates the molecular architecture of the cohesin complex.

Oligo-conjugated antibodies are a powerful tool but require thorough optimization to reduce background signal, increase sensitivity, and reduce sequencing costs.

Cations are essential cofactors in the ATP activation mechanism of the KtrAB cation channel and appear to be cofactors of many other nucleotide-dependent RCK domains.