2,441 results found
    1. Chromosomes and Gene Expression

    Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair

    Natasa Savic et al.
    Linking the DNA repair template to the Cas9 ribonucleoprotein complex enhances homology-directed repair of the induced DNA double strand break.
    1. Structural Biology and Molecular Biophysics
    2. Microbiology and Infectious Disease

    A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5 Å resolution

    Xing Zhang et al.
    Cryo electron microscopy and structure-based mutagenesis reveal that the bacteriophage BPP-1 contains two of the three major recognized viral folds, one of which exhibits a new topology.
    1. Evolutionary Biology

    A Bayesian approach to dynamic homology of morphological characters and the ancestral phenotype of jawed vertebrates

    Benedict King, Martin Rücklin
    The ancestor of all known jawed vertebrates possessed a maxilla and premaxilla and was similar in morphology to the 'maxillate placoderms' from China.
    1. Developmental Biology
    2. Evolutionary Biology

    Embryonic origin and serial homology of gill arches and paired fins in the skate, Leucoraja erinacea

    Victoria A Sleight, J Andrew Gillis
    Development from dual-origin mesenchyme with common competence explains anatomical parallels and shared patterning of the jawed vertebrate gill arch and paired fin skeletons.
    1. Genetics and Genomics

    Multiplexed measurement of variant abundance and activity reveals VKOR topology, active site and human variant impact

    Melissa A Chiasson et al.
    Human vitamin K epoxide reductase (VKOR) has a four transmembrane domain topology that supports the use of a homology model, enabling identification of active site residues and human variant impact.
    1. Developmental Biology
    2. Genetics and Genomics

    Efficient targeted integration directed by short homology in zebrafish and mammalian cells

    Wesley A Wierson et al.
    Short homology arms exposed by CRISPR/Cas9 cleavage can target integration at genomic CRISPR/Cas9 cut sites at high frequencies with reproducible precision using pGTag vectors in zebrafish and mammalian cells.
    1. Chromosomes and Gene Expression

    Enhancement of homology-directed repair with chromatin donor templates in cells

    Grisel Cruz-Becerra, James T Kadonaga
    Precise genome editing by homology-directed repair occurs more efficiently with a chromatin donor template than with a naked DNA donor template.
    1. Microbiology and Infectious Disease

    The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins

    Ashley M Ngo et al.
    The ER membrane protein complex (EMC) facilitates the correct topology of the flavivirus non-structural proteins NS4A and NS4B at the ER membrane critical for viral replication.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Regulation of multispanning membrane protein topology via post-translational annealing

    Reid C Van Lehn et al.
    Coarse-grained modeling reveals a new mechanism for multispanning membrane protein topogenesis, in which misintegrated configurations of the proteins undergo post-translational annealing to reach final, fully integrated multispanning topologies.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    A family of fluoride-specific ion channels with dual-topology architecture

    Randy B Stockbridge et al.
    Efflux of xenobiotic fluoride from microorganisms occurs through a novel family of ion channels with stringent selectivity for fluoride ion and dual-topology molecular architecture.

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