An inorganic tin oxochloride cluster specifically binds to an intrinsically disordered, histidine-rich, low complexity protein region and arrests de novo transcription initiation without affecting reinitiation.
The transcription factor (TF)-binding specificities of Pseudomonas aeruginosa allow us to predict virulence-associated TFs and their target genes, which will facilitate to find effective treatment and prevention for its associated diseases.
Glycolysis is locally enhanced and redirected in zebrafish to generate lactate, which functions as a signaling molecule to fully activate Fgf target genes required for proper sensory and neural development.
Hyung Suk Oh, Werner M Neuhausser ... David M Knipe
CRISPR genome editing technology can efficiently introduce mutations into lytic and latent HSV genomes to block lytic replication and reactivation of latent herpes simplex virus genome though differential mechanisms.
Cloning-free 3Cs technology is developed for the generation of sequence-bias-free covalently closed circular synthesized (3Cs) CRISPR/Cas gRNA libraries that can interrogate the coding and noncoding human genome.
A powerful sequencing approach is used to elucidate the genetic basis of a complex organismal trait, and members of the irld gene family that affect starvation resistance are identified.
Jeroen Overman, Frank Fontaine ... Mathias Francois
A novel molecular strategy to block transcription factor activity using small molecule interference of multiple protein-protein interactions is reported.
A sophisticated classical mutagenesis assay with next-generation sequencing technology presents a benefit to future research on mutagenesis, DNA repair, and cancer.
Nick D Pokorzynski, Amanda J Brinkworth, Rey Carabeo
Analysis of the atypical tryptophan biosynthetic operon of Chlamydia trachomatis revealed the simultaneous regulation of transcriptional initiation and termination by an iron-dependent repressor, expanding known regulatory mechanisms of this pathway.
