Single molecule DNA-binding trajectories and deterministic modeling analyses demonstrate a functional role for high energy partly folded states in Transcription Activator-Like Effectors that could improve future TALEN design.
The transcription factor Pou3f1 triggers embryonic stem cells to become neuronal progenitor cells in two ways: by activating the expression of pro-neuronal genes and by blocking external inhibitory signaling cascades.
Steroid hormone receptors control the expression of their target genes through a digital on-off switch in individual cells, which leads to an analogue dose-response relationship at the level of the whole organism.
Silencing of stem cell identity genes during progenitor commitment ensures that intermediate progenitors robustly commit to generate differentiated cell types rather than abnormal stem-cell-like cells during indirect neurogenesis.
A large-scale RNA interference screen uncovers a new transcriptional regulatory program involving the histone H3 demethylase KDM3A, which mediates detachment-induced apoptosis (anoikis) in breast epithelial cells.
The phosphate starvation response network in a commensal yeast evolved to expand its downstream targets via changes in the main transcription factor's dependence on its co-activator, potentially altering the physiological response.
An atomic model of the 3744-residue Tra1 protein reveals multiple transcription activator binding sites, its integration within the SAGA chromatin coactivator complex, and a striking similarity to DNA-repair factor DNA-PKcs.