Transcriptional activation domains achieve rapid, dynamic, specific interaction with Mediator through binding of an unstructured peptide to multiple hydrophobic surfaces without particular amino acid side chain interactions.
The transcription coactivators SAGA and TFIID have redundant function at a subset of yeast genes while SAGA also contributes to transcription of all genes through regulation of chromatin modifications.
Elucidating the molecular mechanism by which the Hippo signaling effector Yorkie (Yki) functions as a transcriptional coactivator in growth control reveals the importance of a histone-modifying enzyme for this process.
Following fertilization, the pioneering transcription factors GAGA factor (GAF) and Zelda are independently required to reprogram the zygotic genome of Drosophila and activate the first wave of gene expression.
Structural and biochemical analysis reveals that two intrinsically disordered domains of the transcription factor FoxM1 co-fold to form an autoinhibited conformation, which is disrupted by a specific activating phosphorylation event.
Single molecule DNA-binding trajectories and deterministic modeling analyses demonstrate a functional role for high energy partly folded states in Transcription Activator-Like Effectors that could improve future TALEN design.
Steroid hormone receptors control the expression of their target genes through a digital on-off switch in individual cells, which leads to an analogue dose-response relationship at the level of the whole organism.