Sox2 transcription is not correlated with spatial proximity of its essential regulatory enhancer in embryonic stem cells, suggesting gene transcription is not limited to periods of direct enhancer-promoter contact.
Multiple enhancers in physical proximity can reinforce shared transcriptional 'hubs' to preserve their transcriptional output, providing a buffer during environmental stresses and genetic perturbations to preserve phenotypic robustness.
Transcription-factor-dependent noncoding RNA transcription illuminates components of a transcription-factor-dependent gene regulatory network that includes enhancer-associated long noncoding RNAs and is necessary for cardiac rhythm.
A method for measuring p300 chromatin occupancy in specific lineages of mouse tissues was used to map endothelial enhancers and to identify previously unrecognized angiogenesis-related sequence motifs.
A simple and effective method facilitates the study of in vivo transcriptional dynamics using transcriptional enhancers and destabilized fluorescent protein, which is suitable for both live imaging and fixed studies.
Interspecies comparison of transcription factor occupancy during embryogenesis reveals potential co-operative relationships between factors and uncovers the inherent plasticity of developmental enhancers to overcome divergence in transcription factor occupancy.
The sharp expression pattern driven by a classic, simple animal enhancer is determined by multiple molecular mechanisms, not only cooperative binding of the activating transcription factor as was previously thought.