In transgenesis assays involving diploid model organisms, two clearly distinguishable transformation markers embedded in interweaved, but incompatible Lox site pairs allow the systematic creation of homozygous transgenic lines and minimize the number of wasted animals.
Experiments using zebrafish identify a novel sleep-promoting neuronal circuit in which hypothalamic NPVF neurons promote sleep via the hindbrain serotonergic raphe nuclei, which promote sleep in both zebrafish and mice.
Spizellomycespunctatusis a genetically tractable chytrid and model organism for comparative cell biology for understanding evolution of the cell cycle, actin dynamics, and cellularization in fungi and early eukaryotes.
The development of a transgenic zebrafish allows for cell-type-specific labeling of neuronal protein synthesis, enabling brain-wide visualization and quantification of protein synthesis and demonstrating region-specific increases following elevated brain activity.
Morphologic, molecular, biomechanical and computational analyses show that the specialized extracellular matrix architecture of the umbilical artery contributes to its rapid closure at birth and regulates smooth muscle cell differentiation.