4,338 results found
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Novel transgenic pigs with enhanced growth and reduced environmental impact

    Xianwei Zhang, Zicong Li ... Zhenfang Wu
    Transgenic expression of glucanase, xylanase and phytase in pigs enhances growth performance and reduces nitrogen/phosphorus emission, and offers a very valuable biological strategy for sustainable resource utilization and environmental protection in the pork industry.
    1. Immunology and Inflammation

    Characterisation of the immune repertoire of a humanised transgenic mouse through immunophenotyping and high-throughput sequencing

    Eve Richardson, Špela Binter ... Simon J Watson
    A commonly used immunoglobulin loci-transgenic mouse, the Kymouse, has B cells that go through complete development and have receptor repertoires that show key differences in encoding gene frequencies but structural similarity in their most variable regions.
    1. Genetics and Genomics

    High-throughput library transgenesis in Caenorhabditis elegans via Transgenic Arrays Resulting in Diversity of Integrated Sequences (TARDIS)

    Zachary C Stevenson, Megan J Moerdyk-Schauwecker ... Patrick C Phillips
    TARDIS enables efficient and diverse transgenesis in Caenorhabditis elegans by integrating synthetic DNA libraries into engineered genomic sites, rivaling the scale of transformation previously only possible in microbes.
    1. Developmental Biology
    2. Chromosomes and Gene Expression

    A universal vector concept for a direct genotyping of transgenic organisms and a systematic creation of homozygous lines

    Frederic Strobl, Anita Anderl, Ernst HK Stelzer
    In transgenesis assays involving diploid model organisms, two clearly distinguishable transformation markers embedded in interweaved, but incompatible Lox site pairs allow the systematic creation of homozygous transgenic lines and minimize the number of wasted animals.
    1. Immunology and Inflammation

    TCR transgenic clone selection guided by immune receptor analysis and single-cell RNA expression of polyclonal responders

    Nincy Debeuf, Sahine Lameire ... Bart N Lambrecht
    Rationalized selection of responsive TCR clones by single-cell analysis to aid in the generation of novel TCR transgenic animals.
    1. Neuroscience

    Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping

    Kathryn M Tabor, Gregory D Marquart ... Harold A Burgess
    A new resource of transgenic zebrafish lines facilitates precise targeting of neuronal cell-types within the brain, supported by online software for visualizing transgene expression patterns.
    1. Neuroscience

    Impaired astrocytic Ca2+ signaling in awake-behaving Alzheimer’s disease transgenic mice

    Knut Sindre Åbjørsbråten, Gry HE Syverstad Skaaraas ... Rune Enger
    Noradrenaline-mediated astrocytic Ca2+ signaling in behavior is reduced in awake-behaving tg-ArcSwe Alzheimer's disease transgenic mice.
    1. Microbiology and Infectious Disease

    Transgenic shRNA pigs reduce susceptibility to foot and mouth disease virus infection

    Shengwei Hu, Jun Qiao ... Hongsheng Ouyang
    A transgenic small hairpin RNA can provide a viable tool for enhancing resistance to viral infection in livestock and improve animal well-being.
    1. Chromosomes and Gene Expression
    2. Plant Biology

    The MBD7 complex promotes expression of methylated transgenes without significantly altering their methylation status

    Dongming Li, Ana Marie S Palanca ... Julie A Law
    The METHYL-CpG-BINDING DOMAIN 7 (MBD7) complex promotes the activation (rather than repression) of transgenes that undergo DNA methylation and it does so without significantly altering their methylation status, placing this complex downstream of DNA methylation.
    1. Neuroscience

    Establishment of transgenic fluorescent mice for labeling synapses and screening synaptogenic adhesion molecules

    Lei Yang, Jingtao Zhang ... Chen Zhang
    A Syt1-tdTomato transgenic mice with normal synaptic transmission is established to label synapse in situ and screen synaptogenic adhesion molecules.

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