Quantitative binding and kinetic assays on DNA polymerases η and δ define the mechanism for polymerase exchange during human translesion DNA synthesis across a UV-induced lesion on the lagging strand.
Cryo-EM structures of the 30S*RNAP complex visualize co-localization of the transcription and translation machineries and provide insights into the transcription-translation synchrony, which coordinates gene expression in bacteria.
We discuss the methods available to understand lncRNA function in vivo, and highlight important considerations that should be taken into account when designing such experiments.
Biochemical and genetic experiments show that Ebf and Lhx2 cooperate to specify olfactory receptor enhancers, which cooperate to drive olfactory receptor expression.
The unspliced HAC1 mRNA does not give rise to detectable protein in budding yeast, despite its cytoplasmic localization, due to a two-part post-transcriptional silencing mechanism.
Selfish wtf meiotic drive genes use overlapping transcripts to encode both a trans-acting poison to kill gametes that do not inherit the gene and a gamete-autonomous antidote to specifically rescue the gametes that do.
