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  1. Point of View: Competency-based assessment for the training of PhD students and early-career scientists

    Michael F Verderame, Victoria H Freedman ... Wayne T McCormack
    A method to assess the training of scientists, based on a set of 10 core competencies, is proposed.
    1. Ecology
    2. Microbiology and Infectious Disease

    Metabolite exchange between microbiome members produces compounds that influence Drosophila behavior

    Caleb N Fischer, Eric P Trautman ... Nichole A Broderick
    Interactions between fungal and bacterial microbiome members alter Drosophila melanogaster's chemical environment, affecting host behavior and survival.
    1. Cell Biology

    Tissue libraries enable rapid determination of conditions that preserve antibody labeling in cleared mouse and human tissue

    Theodore J Zwang, Rachel E Bennett ... Bradley T Hyman
    Conditions for multiplexed antibody labeling in mouse brain tissue translates to effective labeling in human brain tissue when samples are prepared similarly.
    1. Developmental Biology

    Integration of mouse ovary morphogenesis with developmental dynamics of the oviduct, ovarian ligaments, and rete ovarii

    Jennifer McKey, Dilara N Anbarci ... Blanche Capel
    A detailed characterization of mouse ovary development using tissue clearing and 3D lightsheet imaging identifies ovary folding, specification of cortical and medullary domains, and ovary encapsulation as major hallmarks of ovary morphogenesis.
    1. Neuroscience

    Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain

    Ya-Hui Lin, Li-Wen Wang ... Li-An Chu
    A new approach uses heat-induced antigen retrieval to enable tissue clearing, multiple-round immunostaining, and expansion microscopy for volumetric imaging of centimeter-scale clinical formalin-fixed paraffin-embedded specimens.
    1. Developmental Biology
    2. Neuroscience

    Precise regulation of the guidance receptor DMA-1 by KPC-1/Furin instructs dendritic branching decisions

    Xintong Dong, Hui Chiu ... Kang Shen
    The enzyme KPC-1/Furin down-regulates the DMA-1 receptor in neurons, allowing growing dendrites to migrate away from intermediate targets.
    1. Neuroscience

    Alpha/beta power decreases track the fidelity of stimulus-specific information

    Benjamin James Griffiths, Stephen D Mayhew ... Simon Hanslmayr
    Neural representations of stimulus-specific information increase in fidelity as the power of alpha/beta activity decreases, suggesting that alpha/beta power decreases reflect a domain-general mechanism that supports information representation.
    1. Neuroscience

    Three-dimensional single-cell transcriptome imaging of thick tissues

    Rongxin Fang, Aaron Halpern ... Xiaowei Zhuang
    By integrating multiplexed error-robust fluorescence in situ hybridization (MERFISH), deep learning, optical sectioning, and protocol optimization, thick-tissue 3D MERFISH allows volumetric single-cell transcriptome imaging of thick-tissue samples, broadening the scope of applications of spatial genomics.
    1. Cell Biology
    2. Chromosomes and Gene Expression

    Science Forum: A survey of research quality in core facilities

    Isabelle C Kos-Braun, Björn Gerlach, Claudia Pitzer
    Responses to a survey reveal how core facilities could improve and encourage good research practices.
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Length-dependent disassembly maintains four different flagellar lengths in Giardia

    Shane G McInally, Jane Kondev, Scott C Dawson
    Giardia, a multi-ciliated parasitic protist, maintains four different flagellar lengths by balancing flagellar length-independent IFT-mediated assembly with length-dependent kinesin-13 mediated disassembly.