Aberration correction using 3D microprinting in ultrathin microendoscopes allows two-photon imaging of large neuronal networks with homogeneously high spatial resolution and minimal invasiveness in the deep mouse brain.
Establishment of two-photon imaging with a 1100-nm laser, which underfills the objective's back aperture, detects activity of multiple neurons in the prelimbic area and hippocampal CA1 region of the intact mouse brain.
The combination of a new genetically encoded voltage indicator and fast two-photon imaging methods enables detection of rapid neural electrical activity in organotypic slice cultures and in living flies.
Quantitative experiments and analysis determine the limit of excitation power of 1300-nm three-photon microscopy, and the imaging depth where three-photon outperforms two-photon for calcium imaging in the mouse brain.
The essential role of presynaptic NMDA receptors for granule cell GABAergic output elucidates the function of reciprocal spines in recurrent and possibly lateral inhibition of mitral cells during olfactory processsing.
Simultaneous voltage and calcium two-photon imaging of Purkinje neuron dendrites in awake mice reveals multiple interplaying mechanisms underlying sensory-evoked dendritic coincidence detection of parallel fiber and climbing fiber input.