An inference paradigm for extracting neuronal correlations from two-photon imaging data, without requiring intermediate spike deconvolution, provides significant performance gains over existing methods as demonstrated by theoretical analysis, simulation studies, and real-data applications.

Quantitative experiments and analysis determine the limit of excitation power of 1300-nm three-photon microscopy, and the imaging depth where three-photon outperforms two-photon for calcium imaging in the mouse brain.

Establishment of two-photon imaging with a 1100-nm laser, which underfills the objective's back aperture, detects activity of multiple neurons in the prelimbic area and hippocampal CA1 region of the intact mouse brain.

CaImAn is an open-software package that equips the neuroscience community with a set of turnkey, fast and scalable solutions to pre-processing problems arising in single cell calcium imaging data analysis.

The A1 of ferrets and mice show similar tonotopic organizations, with neurons preferring a single frequency being more precisely organized into a tonotopic map than multipeaked neurons.

Simultaneous calcium imaging and cell-attached recording in GCaMP6 transgenic mice in vivo reveals spiking-fluorescence relationship that supports more refined inference of neuronal activities from calcium imaging data.

Adult-born neurons in the olfactory bulb allow state-dependent pattern separation of representations of similar odorants.

Simultaneous voltage and calcium two-photon imaging of Purkinje neuron dendrites in awake mice reveals multiple interplaying mechanisms underlying sensory-evoked dendritic coincidence detection of parallel fiber and climbing fiber input.

An all-optical 3D two-photon imaging and photostimulation platform was demonstrated, with the capability to precisely stimulate a large group of cells in mice cortex in vivo with low laser power.

A novel deep-learning framework shows how to interpret and decode raw neural recordings, avoiding the need for strong prior hypotheses, revealing a novel representation of head direction.