Quantitative experiments and analysis determine the limit of excitation power of 1300-nm three-photon microscopy, and the imaging depth where three-photon outperforms two-photon for calcium imaging in the mouse brain.
Establishment of two-photon imaging with a 1100-nm laser, which underfills the objective's back aperture, detects activity of multiple neurons in the prelimbic area and hippocampal CA1 region of the intact mouse brain.
CaImAn is an open-software package that equips the neuroscience community with a set of turnkey, fast and scalable solutions to pre-processing problems arising in single cell calcium imaging data analysis.
A distinctive recurrent network motif in the Drosophila central brain enables neurons that encode angular velocity to shift population activity in compass neurons, thereby updating their heading representation whenever the fly turns.
Two photon calcium imaging experiments show that excitatory and inhibitory neurons in the mouse superior colliculus are differentially modulated by the motion contrast between stimulus center and surround.