Quantitative experiments and analysis determine the limit of excitation power of 1300-nm three-photon microscopy, and the imaging depth where three-photon outperforms two-photon for calcium imaging in the mouse brain.
Establishment of two-photon imaging with a 1100-nm laser, which underfills the objective's back aperture, detects activity of multiple neurons in the prelimbic area and hippocampal CA1 region of the intact mouse brain.
CaRuby-Nano is a novel red-emitting and highly sensitive functionalizable calcium indicator with a wide range of applications in neuroscience and cell biology.
CaImAn is an open-software package that equips the neuroscience community with a set of turnkey, fast and scalable solutions to pre-processing problems arising in single cell calcium imaging data analysis.
An all-optical 3D two-photon imaging and photostimulation platform was demonstrated, with the capability to precisely stimulate a large group of cells in mice cortex in vivo with low laser power.
The central complex, a highly conserved insect brain region important for navigation, is characterized by a high degree of recurrence and a sparseness of output pathways.
A distinctive recurrent network motif in the Drosophila central brain enables neurons that encode angular velocity to shift population activity in compass neurons, thereby updating their heading representation whenever the fly turns.
The A1 of ferrets and mice show similar tonotopic organizations, with neurons preferring a single frequency being more precisely organized into a tonotopic map than multipeaked neurons.
Two photon calcium imaging experiments show that excitatory and inhibitory neurons in the mouse superior colliculus are differentially modulated by the motion contrast between stimulus center and surround.
Combining spatial restriction of channelrhodopsin to the neuronal cell body with two photon excitation and calcium imaging will enable production of high resolution maps of neural circuitry.
