Aberration correction using 3D microprinting in ultrathin microendoscopes allows two-photon imaging of large neuronal networks with homogeneously high spatial resolution and minimal invasiveness in the deep mouse brain.
Establishment of two-photon imaging with a 1100-nm laser, which underfills the objective's back aperture, detects activity of multiple neurons in the prelimbic area and hippocampal CA1 region of the intact mouse brain.
The combination of a new genetically encoded voltage indicator and fast two-photon imaging methods enables detection of rapid neural electrical activity in organotypic slice cultures and in living flies.
Quantitative experiments and analysis determine the limit of excitation power of 1300-nm three-photon microscopy, and the imaging depth where three-photon outperforms two-photon for calcium imaging in the mouse brain.
Simultaneous voltage and calcium two-photon imaging of Purkinje neuron dendrites in awake mice reveals multiple interplaying mechanisms underlying sensory-evoked dendritic coincidence detection of parallel fiber and climbing fiber input.
Simultaneous 2-photon imaging of striosomes and matrix in mice shows that striosomes preferentially encode reward-predicting cues whereas both striatal compartments demonstrate reward-related activity.