Establishment of two-photon imaging with a 1100-nm laser, which underfills the objective's back aperture, detects activity of multiple neurons in the prelimbic area and hippocampal CA1 region of the intact mouse brain.
Aberration correction using 3D microprinting in ultrathin microendoscopes allows two-photon imaging of large neuronal networks with homogeneously high spatial resolution and minimal invasiveness in the deep mouse brain.
The combination of a new genetically encoded voltage indicator and fast two-photon imaging methods enables detection of rapid neural electrical activity in organotypic slice cultures and in living flies.
Two photon calcium imaging experiments show that excitatory and inhibitory neurons in the mouse superior colliculus are differentially modulated by the motion contrast between stimulus center and surround.
Simultaneous voltage and calcium two-photon imaging of Purkinje neuron dendrites in awake mice reveals multiple interplaying mechanisms underlying sensory-evoked dendritic coincidence detection of parallel fiber and climbing fiber input.
Quantitative experiments and analysis determine the limit of excitation power of 1300-nm three-photon microscopy, and the imaging depth where three-photon outperforms two-photon for calcium imaging in the mouse brain.