Establishment of two-photon imaging with a 1100-nm laser, which underfills the objective's back aperture, detects activity of multiple neurons in the prelimbic area and hippocampal CA1 region of the intact mouse brain.
The combination of a new genetically encoded voltage indicator and fast two-photon imaging methods enables detection of rapid neural electrical activity in organotypic slice cultures and in living flies.
Two photon calcium imaging experiments show that excitatory and inhibitory neurons in the mouse superior colliculus are differentially modulated by the motion contrast between stimulus center and surround.
Quantitative experiments and analysis determine the limit of excitation power of 1300-nm three-photon microscopy, and the imaging depth where three-photon outperforms two-photon for calcium imaging in the mouse brain.
Simultaneous 2-photon imaging of striosomes and matrix in mice shows that striosomes preferentially encode reward-predicting cues whereas both striatal compartments demonstrate reward-related activity.