1,516 results found
    1. Neuroscience

    In vivo imaging with a water immersion objective affects brain temperature, blood flow and oxygenation

    Morgane Roche et al.
    Brain temperature and oxygenation vary according to the imaging conditions during two-photon microscopy.
    1. Neuroscience

    Mapping oxygen concentration in the awake mouse brain

    Declan G Lyons et al.
    Two-photon phosphorescence lifetime microscopy reveals the physiological values of oxygen concentration and blood flow parameters in the brains of awake mice.
    1. Immunology and Inflammation

    Studying the biology of cytotoxic T lymphocytes in vivo with a fluorescent granzyme B-mTFP knock-in mouse

    Praneeth Chitirala et al.
    A new knock-in mouse endogenously labels cytotoxic granules and can be used with all modern super-resolution imaging techniques to study T cell function in vitro and in vivo.
    1. Neuroscience

    More homogeneous capillary flow and oxygenation in deeper cortical layers correlate with increased oxygen extraction

    Baoqiang Li et al.
    Resting-state capillary blood flow and oxygenation are more homogeneous in the deeper cortical layers, underpinning an important mechanism by which the microvascular network adapts to an increased local oxidative metabolism.
    1. Cell Biology
    2. Immunology and Inflammation

    Intermittent Ca2+ signals mediated by Orai1 regulate basal T cell motility

    Tobias X Dong et al.
    Orai1 channels activate intermittently in motile T cells, generating calcium transients, detected by a novel genetically encoded indicator and inducing pauses that favor antigen scanning.
    1. Developmental Biology
    2. Neuroscience

    Frazzled promotes growth cone attachment at the source of a Netrin gradient in the Drosophila visual system

    Orkun Akin, S Lawrence Zipursky
    Direct observation in the developing fly brain indicates that the response of a growth cone in a gradient of a guidance cue is adhesion to the source of the cue rather than attraction by the gradient.
    1. Neuroscience

    Laser ablation of Dbx1 neurons in the pre-Bötzinger complex stops inspiratory rhythm and impairs output in neonatal mice

    Xueying Wang et al.
    Laser-ablating neurons of a single genetic class reveals that Dbx1-derived interneurons comprise core respiratory rhythmogenic and premotor circuits and provides quantitative cellular parameters that govern network functionality
    1. Cell Biology
    2. Immunology and Inflammation

    T-cell calcium dynamics visualized in a ratiometric tdTomato-GCaMP6f transgenic reporter mouse

    Tobias X Dong et al.
    Salsa6f is a novel, ratiometric genetically encoded Ca2+ indicator that combines the power of ratiometric chemical Ca2+ indicators with the ability, when expressed transgenically, to image cellular Ca2+ signals amid complex tissue environments in vivo.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Quantitative analysis of 1300-nm three-photon calcium imaging in the mouse brain

    Tianyu Wang et al.
    Quantitative experiments and analysis determine the limit of excitation power of 1300-nm three-photon microscopy, and the imaging depth where three-photon outperforms two-photon for calcium imaging in the mouse brain.

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