Clathrin-coated vesicles can be made to form on intracellular membranes using a minimal molecular system, which defines the core vesicle budding machinery.

The first quantitative live-cell microscopy this work shows depicting the order of events preceding secretion in budding yeast.

Prominin 1 and Tweety Homology 1, two proteins that regulate membrane shape in neural and neuroepithelial cells, have shared evolutionary history and both cause cells to secrete extracellular vesicles.

The interplay between the Flower Ca²⁺ channel and PI(4,5)P₂ spatiotemporally couples synaptic vesicle exocytosis to activity-dependent bulk endocytosis and synaptic vesicle reformation from bulk endosomes.

Analysis of neurons that lack the two neuronal dynamins, dynamin 1 and 3, demonstrates a pathway of synaptic vesicle reformation that does not require these two dynamins or clathrin-dependent budding.

Chemical labelling of all primary endocytic vesicles allows the contribution of different endocytic mechanisms to the total endocytic uptake in cultured mammalian cells to be quantified.

Whole-transcriptomic profiling of circulating small extracellular vesicle RNA facilitates the detection of T1a stage colorectal cancer and precancerous advanced adenoma.

Parallel measurements of pH gradient and membrane potential at the single vesicle level have revealed that the synaptic vesicle acidification is initiated by removal of its clathrin coat, which blocks vesicular ATPase activity.

There are two distinct modes of late endosome fusion in the mouse yolk sac visceral endoderm cells and actin dynamics plays a role in regulating vesicle fusion.