Ca2+ channels and release sensors at a fast central synapse are tightly coupled, which minimizes the effect of extracellular Ca2+ concentration on the timing of transmitter release.
Morayma M Temoche-Diaz, Matthew J Shurtleff ... Randy Schekman
Biochemical fractionation of vesicle sub-populations and in vitro reconstitution studies reveal that Lupus La protein mediates the selective sorting of miR-122 into extracellular vesicles in vitro and in vivo.
An unbiased model for the self-organisation of the Golgi apparatus displays either anterograde vesicular transport or cisternal maturation depending on ratios of budding, fusion and biochemical conversion rates.
Arpiar Saunders, Adam J Granger, Bernardo L Sabatini
Neurons of the cholinergic system, which release the excitatory neurotransmitter acetycholine throughout the cortex, also release the inhibitory transmitter GABA, with potential implications for cognitive function.
Proximity proteomics combined with mouse genetics are used to assess the composition of active zone-like sites for dopamine release and reveal that the scaffolding protein RIM organizes these sites.
Brooks G Robinson, Xintong Cai ... Pascal S Kaeser
Midbrain dopamine neurons use sophisticated secretory machinery to establish specialized sites for action potential-evoked release of dopamine from their cell bodies and dendrites.
Andrea Salazar Lázaro, Thorsten Trimbuch ... Christian Rosenmund
Structure–function studies on the synaptic vesicle release protein syntaxin-1 identified structural motifs on the surface of the SNARE complex controlling vesicle priming and spontaneous release unique for synaptic signaling.
Maria I Lazaro-Pena, Adam B Cornwell ... Andrew V Samuelson
The transcriptional cofactor HPK-1 (homeodomain-interacting protein kinase) functions as a key regulator of multiple proteostatic stress responses, each originating from discrete neuronal subtypes within the Caenorhabditis elegans nervous system to preserve neuronal health and maintain organismal proteostasis during normal aging.
GABAergic neurons in the nervous system of C. elegans have been comprehensively mapped allowing analysis of regulatory factors that program GABAergic neuron identity.
Co-release of the functionally opposing fast neurotransmitters, glutamate and GABA, from distinct synaptic vesicles within the same supramammillary synaptic terminal modulates dentate granule cell firing in a frequency-dependent manner.
