40 results found
    1. Structural Biology and Molecular Biophysics

    The isolated voltage sensing domain of the Shaker potassium channel forms a voltage-gated cation channel

    Juan Zhao, Rikard Blunck
    Expression of the isolated voltage sensing domain significantly alters its structural conformation as well as its gating kinetics, indicating the importance of studying the biological assembly in its entirety.
    1. Structural Biology and Molecular Biophysics
    2. Neuroscience

    Single-molecule fluorimetry and gating currents inspire an improved optical voltage indicator

    Jeremy S Treger et al.
    ArcLight, a popular optogenetic reporter of voltage, is studied at both single-molecule and macroscopic levels, which leads to new mechanistic understanding and to the rational design of a faster reporter.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Cellular encoding of Cy dyes for single-molecule imaging

    Lilia Leisle et al.
    Cyanine fluorophores are encoded as non-canonical amino acids to produce functional proteins in cell-free translation systems and live cells for single-molecule imaging.
    1. Structural Biology and Molecular Biophysics

    Helix breaking transition in the S4 of HCN channel is critical for hyperpolarization-dependent gating

    Marina A Kasimova et al.
    The gating polarity of a voltage-gated ion channel is primarily determined by turn propensity of residues at a critical position in the middle of the S4 voltage-sensing helix.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Visualizing conformational dynamics of proteins in solution and at the cell membrane

    Sharona E Gordon et al.
    ACCuRET is a flexible new method for measuring the structural dynamics of proteins in solution and membrane proteins in their native environment.
    1. Structural Biology and Molecular Biophysics

    Gating and selectivity mechanisms for the lysosomal K+ channel TMEM175

    SeCheol Oh et al.
    Structures of the non-canonical potassium channel TMEM175 in open and closed states reveal unique mechanisms for channel gating and the selective permeation of K+ ions.
    1. Neuroscience

    Morphine disinhibits glutamatergic input to VTA dopamine neurons and promotes dopamine neuron excitation

    Ming Chen et al.
    Experiments examining the actions of morphine on single neurons from one of the brain's reward centers, the VTA, reveal the mechanism by which the drug exerts its hedonic effects.
    1. Cell Biology

    Bax and Bak function as the outer membrane component of the mitochondrial permeability pore in regulating necrotic cell death in mice

    Jason Karch et al.
    The proteins Bax and Bak, which increase the permeability of the mitochondrial membrane during apoptosis, are also crucial for generating a mitochondrial membrane pore that is specifically involved in necrosis.
    1. Structural Biology and Molecular Biophysics

    Structure and dynamics underlying elementary ligand binding events in human pacemaking channels

    Marcel P Goldschen-Ohm et al.
    Single-molecule resolution of cAMP binding to the ligand binding domain of pacemaking channels in zero-mode waveguides reveals the dynamics of the distinct steps underlying both binding and isomerization of the binding domain.
    1. Physics of Living Systems

    Mapping out Min protein patterns in fully confined fluidic chambers

    Yaron Caspi, Cees Dekker
    The geometry selection rules of dynamic Min protein patterns are determined in fully confined fluidic chambers, showing that both oscillations and running waves are derivatives of spiral rotations that are established as the majority pattern.

Refine your results by:

Type
Research categories