A computational model of a yeast chromosome, based on first principles, recapitulates in vivo chromosome behavior, and thus provides unprecedented insight into what the inside of a chromosome is likely to look like.
Substitutions in general translation initiation factor eIF1A found as recurring somatic mutations in uveal melanoma destabilize the closed conformation of the preinitiation complex at the start codon and increase discrimination against suboptimal initiation codons genome-wide.
In yeast, a fluorescent vacuolar cargo departs from Golgi cisternae early in the TGN stage of maturation, and traffic from prevacuolar endosomes to the vacuole apparently involves a kiss-and-run mechanism.
Yeast promoters can harbor multiple natural DNA variants that influence gene expression, interact genetically, evolve under negative selection, alter transcription factor motifs, and remain challenging to predict.
A new high-throughput method for single-cell RNA-seq in yeast cells shows how stochastic expression of glucose-repressed genes contributes to cell-to-cell differences during adaptation to an environmental change.