1. Computational and Systems Biology
  2. Evolutionary Biology

Point of View: Biological causal links on physiological and evolutionary time scales

  1. Amit Karmon
  2. Yitzhak Pilpel  Is a corresponding author
  1. Weizmann Institute of Science, Israel
https://doi.org/10.7554/eLife.14424
Share this article
Cite this article
  1. Amit Karmon
  2. Yitzhak Pilpel
(2016)
Point of View: Biological causal links on physiological and evolutionary time scales
eLife 5:e14424.
https://doi.org/10.7554/eLife.14424
  2. Download BibTeX
  3. Download .RIS

Abstract

Correlation does not imply causation. If two variables, say A and B, are correlated, it could be because A causes B, or that B causes A, or because a third factor affects them both. We suggest that in many cases in biology, the causal link might be bi-directional: A causes B through a fast-acting physiological process, while B causes A through a slowly accumulating evolutionary process. Furthermore, many trained biologists tend to consistently focus at first on the fast-acting direction, and overlook the slower process in the opposite direction. We analyse several examples from modern biology that demonstrate this bias (codon usage optimality and gene expression, gene duplication and genetic dispensability, stem cell division and cancer risk, and the microbiome and host metabolism) and also discuss an example from linguistics. These examples demonstrate mutual effects between the fast physiological processes and the slow evolutionary ones. We believe that building awareness of inference biases among biologists who tend to prefer one causal direction over another could improve scientific reasoning.

Main text

More than 50 years ago Ernst Mayr introduced the distinction between “proximate” and “ultimate” causes in biology (Mayr, 1961). In this now classical theory, proximate causes act via short-term physiological processes, and ultimate causes act via long-term evolutionary processes. Put simply, the proximate cause addresses the question of “how?” and the ultimate cause addresses the “why?”.

The extensive data yielded by modern biology are full of intriguing correlations between variables. A great conceptual challenge, which often triggers intense arguments, is to decipher the nature of causal links that underlie these observations. It seems to us that biologists tend to interpret causation in a way that reflects their education and other biases, often noticing at first the proximate cause. We follow with a few examples of biological questions in which the direction of a causal link (physiological or evolutionary) has been, or could have been, debated. In each example we describe how a physiological causal link (“A causes B”) could account for an observed correlation, but also how the opposite causal direction (“B causes A”), which takes place over an evolutionary time scale, can and perhaps should be invoked. We conclude with a discussion on the interaction between the fast-acting and slow-acting effects in biology and ask why biologists might intuitively focus on one effect over another.

Codon usage optimality and gene expression

Let’s first consider the often-observed correlation between the codon usage of a gene and its protein expression level (Sharp and Li, 1987). Scientists who wish to overexpress a gene in a heterologous system know that they need to “codon-optimize” their gene. That is to say, they must alter its coding sequence so that it is the best match for the codon usage of the most highly expressed genes in the host genome. These are often the codons that correspond to abundant tRNAs in that species. Indeed the effects of codon optimization of gene expression are most pronounced in heterologous expression systems (see Gustafsson et al., 2004). Yet also for the natural genes in a genome, the most codon-optimized genes are often most highly used and expressed (Sharp and Li, 1987; Man and Pilpel, 2007). This may lead to the conclusion that optimal codon usage is a cause of high protein expression for natural genes too. In other words, codon optimization is a proximate cause for protein expression level.

Many causal links in biology might be caused by fast-acting physiological processes acting in one direction and slower evolutionary processes acting in the opposite direction. 

Illustration: Claudia Stocker, vividbiology.com

But could the opposite causal direction provide additional explanation of the observed correlation? Let’s state clearly the potential opposite effect: “a high level of expression of a protein (that may have been achieved via various mechanisms) affects the codon optimality of its gene”. Is that possible? On an evolutionary time scale, maybe it is and protein expression level is an ultimate cause of codon optimality over millions of years (Kudla et al., 2009). It is plausible that highly expressed genes are selected more vigorously for codon optimization than lowly expressed genes, because the fitness cost of not optimizing them (in terms of a burden on the translation apparatus) is likely to be higher. We can even imagine that highly expressed genes could affect the translation machinery (e.g. the tRNA pool) to become more optimally suited to their codons, and thus force the genome to define their codon usage as “optimal” (Yona et al., 2013).

While the evolutionary causal direction in this example was well appreciated early on mainly by evolutionary biologists (Ikemura, 1981; see Plotkin et al. 2011 for a summary), for others the physiological causal direction was the first to be envisaged (Sharp et al., 1986).

Gene duplication and genetic dispensability

Our second example has to do with gene duplication, and the essentiality of the same genes. Gene duplication has without doubt played a major role in the evolution of biological complexity and has served as a source of genetic novelty (Ohno et al., 1968; Lynch and Conery, 2000). Notwithstanding, a well-known observation is that genes that have “duplicates” (or paralogs) in the genome often appear to be less essential, compared to genes that occur as a single copy (Gu et al., 2003). An intuitive interpretation of this correlation, that was shown to be correct in several cases, suggests that a gene is non-essential because its duplicate can compensate if it is mutated (Kafri et al., 2005, DeLuna et al., 2010). In other words, duplication causes dispensability. But could it be that dispensability causes duplication too? How could that happen?

Consider the possibility that “dispensable” genes, defined as those that can be deleted without reducing the fitness of the organism, may also be tolerated at a duplicated dosage. On the other hand, “essential” genes, whose copy number cannot be reduced to zero, may also not tolerated at more than one copy (He and Zhang, 2006). According to this possibility, if a gene is dispensable, the chances that evolution will tolerate its duplication are higher compared to genes that carry out more essential functions. Please note that we do not intend to judge the plausibility of this alternative evolutionary explanation here (see Discussion below), but instead we want to show that this alternate explanation is certainly of interest and yet it took longer to appear.

What is common to the codon usage and gene essentiality examples above? Both feature a strong correlation observed throughout genomes. In both cases, the originally proposed effect, is perhaps more intuitive to many molecular biologists and describes a physiological process that typically occurs on a time scale of minutes, hours or, at the most, days (i.e., protein expression and compensation between genes). In contrast, the alternatives – “high expression causes codon optimality” and “gene dispensability causes duplication” – both describe processes that could occur on a time scale of millions of years or more.

Let’s now take two additional examples from biology, and then one from linguistics.

Stem cell division and cancer risk

Recently, the number of stem cell divisions within a tissue or organ in humans was shown to correlate strongly with the lifetime risk of cancer arising within that body part (Tomasetti and Vogelstein, 2015). The interpretation given to this finding was that each stem cell division has a certain probability to result in a carcinogenic mutation such that tissues that divide more have a greater chance of developing these mutations. Essentially this interpretation proposes that stem cell division affects risk of cancer. But can we also consider the flip side of this effect?

In a tissue with a cell count that remains steady, a high rate of stem cell divisions should be balanced with a high rate of cell death. Suppose also that certain tissues are more prone than others to becoming cancerous simply because, for example, they are commonly exposed to environmental damage that could mutate the DNA in the non-dividing cells. We would expect such tissues, which would include the skin and those in the gut, to have evolved mechanisms to defend themselves against cancer. Maybe instead of stem cell division affecting risk of cancer we are seeing the results of such a protective mechanism. That is to say, high-risk tissues feature high rates of cellular turnover, with extensive programmed cell death to eliminate potentially mutated cells and many stem cell divisions to compensate for the loss of cells.

Interestingly, in this example, the physiological causal link again appears to have been more intuitive for many biologists, and the evolutionary one is provided here as an additional possibility for further discussion. Of course, this proposed mechanism might have a cost too, because more stem cell divisions means more DNA replication and an increased risk of cancerous mutations. Nevertheless, perhaps this cost is worth paying to eliminate the cells damaged by the environment.

The microbiome and host metabolism

Ground-breaking experiments on microbiome manipulation in mice showed that colonization of germ-free mice with a microbiota of obese donors results, within a couple of weeks, in a greater increase in total body fat compared to colonization with a 'lean microbiota' (Turnbaugh et al., 2006). These experiments showed that the microbiota can affect the host metabolic environment, perhaps for their own benefit (see also McNally and Brown, 2015). Put in simpler terms, the microbiota composition might cause host obesity, potentially serving the microbes' needs. Importantly, the seminal work by Turnbaugh et al. went well beyond merely observing a correlation; it also established a direct causal link through experimental manipulation.

Notwithstanding the validity of this study, and in no way implying that the original causal link is wrong, what if we were to also consider a slow-acting effect in the opposite direction? Could a host’s inner metabolic environment select for certain microbes? This possibility opens up others. “Obese” or “lean” microbiota might thrive in “obese” or “lean” hosts, and thus they might have evolved adaptive mechanisms to help design a comfortable environment for themselves. In addition, other factors in a person’s metabolism might determine which germs will eventually colonize them. In other words, perhaps your body selects its microbiota, which in turn feedbacks onto your metabolism. This would be a slow evolutionary effect, at least in terms of microbial evolutionary time scales.

What experiments would support this conjecture? Maybe the above-mentioned experiment of microbiota transfer might provide an answer if it was extended over months or years. For example, take an originally lean mouse, make a germ-free version of it, and transplant into it the microbiota of either a lean or an obese mouse donor. Then, follow the stability of the transplants for prolonged periods of time. If metabolic environment affects the microbiome, then this originally lean mouse should more stably sustain the donation from the lean mouse. Interestingly, this alternative causal direction is supported by a recent study that compared the microbiome of hundreds of human twins and concluded “host genetics influence the composition of the human gut microbiome and can do so in ways that impact host metabolism” (Goodrich et al., 2014). Again in this example, it is important to note that the originally proposed effect occurs within a short-term physiological time frame, yet a plausible effect in the opposite direction might play out over a microbial evolutionary time scale.

Language and perception

Our final example is one from language and linguistics. The principle of “linguistic relativity” holds that the structure of a language affects the ways in which its speakers conceptualize their world, or, put more simply: “we think in the way that we speak”. There is circumstantial evidence that serves to provide support for this notion. For example, native speakers of the Pirahã language, which does not have names for numbers above two, perform relatively poorly in simple counting tasks (Everett, 2005); natives of languages in which directions are absolute (“south”, “north,” “east” and “west”) rather than relative (“right” or “left”) navigate better (Boroditsky, 2011); and natives of languages such as Russian, in which different shades of blue have different names, can distinguish between such colours more easily (Winawer et al., 2007). These and other examples indeed suggest that the way people speak affects the way they think, or perceive the world. Yet here too, we could consider an effect in the opposite direction. Put explicitly: maybe the way we think or perceive the world affects the way our language evolves. The linguist John McWhorter also strongly argues against “linguistic relativity,” suggesting that, despite differences between languages, “the world looks the same in any language.

Like in the above examples from biology, there are two potential directions of effect that could explain correlations between language and perception, and they would act on either a short time scale or a long time scale. The putative effect of perception on language would necessarily take place over an evolutionary time scale, while effects of language on perception would occur during our life span as we acquire a language. For example, is it possible that the language of the Aboriginal group Kuuk Thaayorre, which uses absolute directions, was shaped in its rather peculiar way because absolute directions were more evolutionarily critical than relative directions in that culture? That is, maybe this group, to which navigation through absolute directions comes naturally, survived better over the years, and their language simply reflects their natural inclinations. Likewise, the different names for shades of blue in Russian might reflect a peculiarity in the genes that determine colour vision in the Russian population. Although we will leave the resolution of this general dispute to the linguists, it is interesting to notice that, in this example too, the observed correlation between language and perception might be interpreted as indicating a bi-directional causal link.

Discussion

The five examples described above each involve a pair of variables which are correlated. In each case, a short-term effect that acts on a physiological time scale seemed more intuitive at first, at least for traditionally trained molecular biologists. Curiously, the slower-acting potential effect that requires involvement of evolutionary processes was less intuitive, albeit typically not to evolutionary biologists. We note though that under certain conditions a bi-directional effect, both physiological and evolutionary might be at work. In particular, consider again A and B as two characteristics of an organism. First, suppose that B is a characteristic that increases an organism’s fitness (e.g. high expression of a gene). Second, suppose that the organism can display B without the characteristic A (e.g. high codon optimality of that gene), but that A makes B more efficient. Then, after a long evolutionary process, we would expect the organism to develop a mechanism in which B is established through the means of A. This means that the effect “B causes A” depends on the existing effect “A causes B”. For example, evolution likely selected for high codon optimality of highly expressed genes (Kudla et al., 2009). But selection for optimal codons could only come into play more effectively in highly expressed genes if codon optimization affected gene expression in the first place.

Let us return to Mayr’s classic that introduced the distinction between proximate and ultimate causal explanations (Mayr, 1961). This distinction has been debated over the years. Some have argued later that the distinction is not always a sharp one, and that “proximate mechanisms both shape and respond to selection” (Laland et al., 2011). Without committing ourselves to any claim about the validity, sharpness or universality of this distinction, we agree that sometimes two variables can be the proximate and the ultimate causes of one another. While A is a proximate cause of B, B may have prevailed even before A, and may have ultimately affected A.

So why is the reasoning of many biologists seemingly more prone to focus at first on the effect acting on the short-term, physiological time scale explanation and not on the processes that take millions of years to manifest themselves? Is it because of the biologists’ training? Or is it because it’s easier to think in terms of attainable laboratory experiments? Other reasoning concepts and tools of course must be exercised too. For example, Occam’s Razor – the problem solving principle in philosophy that suggests that the theory with fewest assumptions should be preferred at first – could be helpful here in judging each potential causal direction. For example in the gene duplication and genetic dispensability problem presented above, the evolutionary causal effect requires the extra assumption that dispensable genes are more likely to undergo gene duplication during evolution; this is non-trivial.

In any case, it is interesting to realize that our reasoning is influenced by certain biases that might prevent us, in some cases, from seeing the full picture. We hope that an awareness of such pitfalls could help others to fix or even avoid them.

References

  16. Book
    1. McWhorter JH
    (2014)
    The Language Hoax: Why the World Looks the Same in Any Language
    Oxford University Press.

Decision letter

  1. Stuart King
    Reviewing Editor; eLife, United Kingdom

In the interests of transparency, eLife includes the editorial decision letter and accompanying author responses. A lightly edited version of the letter sent to the authors after peer review is shown, indicating the most substantive concerns; minor comments are not usually included.

Thank you for submitting your article "Biological causal links on physiological and evolutionary time scales" to eLife for consideration as a Feature Article. Two peer reviewers have reviewed your article, and an Associate Features Editor – Stuart King – and the Features Editor – Peter Rodgers – have overseen the evaluation.

The Associate Features Editor has drafted this consolidated decision letter to help you prepare a revised submission.

Summary:

Karmon and Pilpel present a highly unusual opinion piece that reads more like a scientist's musings or even a stream of consciousness, rather than as a comprehensive review of a topic. However, as unusual as this contribution is, it does make a noteworthy and thought-provoking point: that evolutionary explanations of biological causality are often overlooked by researchers focusing on fast-acting physiological explanations.

The five sub-sections selected by Karmon and Pilpel are also varied: some cover well-trodden ground (but are nonetheless worth repeating), while others seem almost entirely new. For example, the sub-section about stem cell division and cancer risk seems to be a mostly new idea that has not been discussed by evolutionary biologists so much or as long as codon usage and gene duplication; it thus provides good contrast in the article.

Together it is an interesting read, but lacks scholarship and connection to the prior literature. The following issues need to be remedied in the revision.

1) Connection to the prior literature

1A) More than fifty years ago, Ernst Mayr wrote about the nature of causation in biology, and distinguished between proximate (physiology) and ultimate causes (evolution). Since then, others have noted that this rigorous distinction is problematic to say the least, and that reciprocal causation may now prove useful for many specific biological problems (Laland et al. 2011; DOI: 10.1126/science.1210879). Since your paper attempts to discuss well-defined segments of this problem, you should put your arguments and speculation within the proper historical context.

1B) A few other important citations are missing from specific sub-sections. In the gene duplication example, a reference to Ohno (e.g. S. Ohno, Evolution by Gene Duplication, Springer 1970) is critical but missing, and a reference to Lynch's work on gene duplication and genome evolution (e.g. M. Lynch, The Origins of Genome Architecture, Sinauer 2007) should be added too. In the microbiome example, a reference to the large evolutionary literature on "niche construction" would be useful.

2) Consideration of problem-solving principles

At the end of the paper, you ask "why is the reasoning of many biologists, so it seems, more prone to prefer the effect acting on the short-term, physiological time scale explanation over the processes that take millions of years to manifest themselves?" The brief discussion that follows could be improved if it also considered how general problem-solving principles, such as Occam's razor, could explain the 'bias'.

For example, in the gene duplicability sub-section – the pattern that needs to be explained is that duplicated genes in the genome often appear to be less essential. There are two, mutually non-exclusive explanations:

i) The presence of a back-up gene mitigates fitness effect of mutations in the other copy.ii) Gene dispensability drives gene duplication on an evolutionary timescale.

Both hypotheses are relevant, but there is a good reason why researchers should start by focusing on the first hypothesis. This is because the second hypothesis has an extra assumption (that dispensable genes are more likely to undergo gene duplication during evolution), which is non-trivial and has relatively little empirical basis. The first hypothesis has no such hidden assumption, and testing its predictions is straightforward (e.g. by comparing the fitness of singleton and double-knock-outs). Therefore, only if the predictions of this first hypothesis fail would most people consider the more complicated models that assume that evolution (i.e. gene duplicability) differs across gene classes. By not taking this into consideration, there is a risk that the current manuscript could potentially encourage readers to ignore simple null models.

3) Example of codon usage

The example about codon usage is perhaps the best illustration of the overall point made in the piece. However, the article should make it clearer that the fast-acting physiological effect is generally only visible in the unnatural case of a highly expressed transgene. By contrast, the effects of codon usage on synthesis for endogenous genes are much weaker, even for the most strongly expressed endogenous genes, which is why it has taken evolutionary timescales for the genome to adjust codon usage (and has done so only for high-expression endogenous genes).

https://doi.org/10.7554/eLife.14424.001

Author response

The following issues need to be remedied in the revision. 1) Connection to the prior literature 1A) More than fifty years ago, Ernst Mayr wrote about the nature of causation in biology, and distinguished between proximate (physiology) and ultimate causes (evolution). Since then, others have noted that this rigorous distinction is problematic to say the least, and that reciprocal causation may now prove useful for many specific biological problems (Laland et al. 2011; DOI: 10.1126/science.1210879). Since your paper attempts to discuss well-defined segments of this problem, you should put your arguments and speculation within the proper historical context.

Thank you for this essential point. We now made explicit reference to Mayr’s classic, once at the Introduction, it now opens our manuscript by introducing the terms proximate and ultimate, and then again in the Discussion, where we also discuss Laland’s reflection. See “More than 50 years ago Ernst Mayr…” and “Let us return back to Mayr’s classic”.

1B) A few other important citations are missing from specific sub-sections. In the gene duplication example, a reference to Ohno (e.g. S. Ohno, Evolution by Gene Duplication, Springer 1970) is critical but missing, and a reference to Lynch's work on gene duplication and genome evolution (e.g. M. Lynch, The Origins of Genome Architecture, Sinauer 2007) should be added too. In the microbiome example, a reference to the large evolutionary literature on "niche construction" would be useful.

Correct. We now reference Ohno and Lynch in the gene duplication section, and provide a representative recent mention of niche construction, which we connect to our argument (see “Gene duplication, no doubt, has played…” and These experiments showed that the microbiota can affect the host metabolic environment, perhaps for their own benefit (see also McNally and Brown, 2015)). We also reference codon optimization in the context of heterologous gene expression (see point 3) below).

2) Consideration of problem-solving principles At the end of the paper, you ask "why is the reasoning of many biologists, so it seems, more prone to prefer the effect acting on the short-term, physiological time scale explanation over the processes that take millions of years to manifest themselves?" The brief discussion that follows could be improved if it also considered how general problem-solving principles, such as Occam's razor, could explain the 'bias'. For example, in the gene duplicability sub-section – the pattern that needs to be explained is that duplicated genes in the genome often appear to be less essential. There are two, mutually non-exclusive explanations:

i) The presence of a back-up gene mitigates fitness effect of mutations in the other copy.ii) Gene dispensability drives gene duplication on an evolutionary timescale.

Both hypotheses are relevant, but there is a good reason why researchers should start by focusing on the first hypothesis. This is because the second hypothesis has an extra assumption (that dispensable genes are more likely to undergo gene duplication during evolution), which is non-trivial and has relatively little empirical basis. The first hypothesis has no such hidden assumption, and testing its predictions is straightforward (e.g. by comparing the fitness of singleton and double-knock-outs). Therefore, only if the predictions of this first hypothesis fail would most people consider the more complicated models that assume that evolution (i.e. gene duplicability) differs across gene classes. By not taking this into consideration, there is a risk that the current manuscript could potentially encourage readers to ignore simple null models.

Thank you for a very good point. We now refer to Occam’s Razor as an additional concept and tool in prioritizing between theories (causal effects, in our case) and indeed follow this exact argument to show how it could be used to support one causal direction over then other (see “Other reasoning concepts and tools of course must be exercised too. For example, Occam’s Razor…”). I believe that with that said the reader might easily apply these reasoning tools to additional problems.

3) Example of codon usage The example about codon usage is perhaps the best illustration of the overall point made in the piece. However, the article should make it clearer that the fast-acting physiological effect is generally only visible in the unnatural case of a highly expressed transgene. By contrast, the effects of codon usage on synthesis for endogenous genes are much weaker, even for the most strongly expressed endogenous genes, which is why it has taken evolutionary timescales for the genome to adjust codon usage (and has done so only for high-expression endogenous genes).

We agree with the referee and indeed now clarify that the physiological direction applies mainly for heterologous expression systems (“Indeed the effects of codon optimization of gene expression are most pronounced in heterologous…”).

https://doi.org/10.7554/eLife.14424.002

Article and author information

Author details

  1. Amit Karmon

    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-0763-6696

  2. Yitzhak Pilpel

    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    For correspondence
    Pilpel@weizmann.ac.il
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-3200-9344

Acknowledgements

The authors thank the Minerva Center for Live Emulation of Evolution in the Lab for grant support.

Publication history

  1. Received:
  2. Accepted:
  3. Version of Record published:

Copyright

© 2016, Karmon et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 9,542
    views
  • 948
    downloads
  • 11
    citations

Views, downloads and citations are aggregated across all versions of this paper published by eLife.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Amit Karmon
  2. Yitzhak Pilpel
(2016)
Point of View: Biological causal links on physiological and evolutionary time scales
eLife 5:e14424.
https://doi.org/10.7554/eLife.14424

Categories and tags

    1. Of interest

    Mitochondrial respiration atlas reveals differential changes in mitochondrial function across sex and age

    Dylan C Sarver, Muzna Saqib ... G William Wong
  2. Further reading

Further reading

    1. Computational and Systems Biology

    Mitochondrial respiration atlas reveals differential changes in mitochondrial function across sex and age

    Dylan C Sarver, Muzna Saqib ... G William Wong
    Research Article

    Organ function declines with age, and large-scale transcriptomic analyses have highlighted differential aging trajectories across tissues. The mechanism underlying shared and organ-selective functional changes across the lifespan, however, still remains poorly understood. Given the central role of mitochondria in powering cellular processes needed to maintain tissue health, we therefore undertook a systematic assessment of respiratory activity across 33 different tissues in young (2.5 months) and old (20 months) mice of both sexes. Our high-resolution mitochondrial respiration atlas reveals: (1) within any group of mice, mitochondrial activity varies widely across tissues, with the highest values consistently seen in heart, brown fat, and kidney; (2) biological sex is a significant but minor contributor to mitochondrial respiration, and its contributions are tissue-specific, with major differences seen in the pancreas, stomach, and white adipose tissue; (3) age is a dominant factor affecting mitochondrial activity, especially across most brain regions, different fat depots, skeletal muscle groups, eyes, and different regions of the gastrointestinal tract; (4) age effects can be sex- and tissue-specific, with some of the largest effects seen in pancreas, heart, adipose tissue, and skeletal muscle; and (5) while aging alters the functional trajectories of mitochondria in a majority of tissues, some are remarkably resilient to age-induced changes. Altogether, our data provide the most comprehensive compendium of mitochondrial respiration and illuminate functional signatures of aging across diverse tissues and organ systems.

    1. Computational and Systems Biology

    Cellular Respiration: Mapping mitochondrial aging

    Alessandro Bitto
    Insight

    Measuring mitochondrial respiration in frozen tissue samples provides the first comprehensive atlas of how aging affects mitochondrial function in mice.

    1. Computational and Systems Biology

    Statistical analysis supports pervasive RNA subcellular localization and alternative 3' UTR regulation

    Rob Bierman, Jui M Dave ... Julia Salzman
    Research Article

    Targeted low-throughput studies have previously identified subcellular RNA localization as necessary for cellular functions including polarization, and translocation. Furthermore, these studies link localization to RNA isoform expression, especially 3’ Untranslated Region (UTR) regulation. The recent introduction of genome-wide spatial transcriptomics techniques enables the potential to test if subcellular localization is regulated in situ pervasively. In order to do this, robust statistical measures of subcellular localization and alternative poly-adenylation (APA) at single-cell resolution are needed. Developing a new statistical framework called SPRAWL, we detect extensive cell-type specific subcellular RNA localization regulation in the mouse brain and to a lesser extent mouse liver. We integrated SPRAWL with a new approach to measure cell-type specific regulation of alternative 3’ UTR processing and detected examples of significant correlations between 3’ UTR length and subcellular localization. Included examples, Timp3, Slc32a1, Cxcl14, and Nxph1 have subcellular localization in the mouse brain highly correlated with regulated 3’ UTR processing that includes the use of unannotated, but highly conserved, 3’ ends. Together, SPRAWL provides a statistical framework to integrate multi-omic single-cell resolved measurements of gene-isoform pairs to prioritize an otherwise impossibly large list of candidate functional 3’ UTRs for functional prediction and study. In these studies of data from mice, SPRAWL predicts that 3’ UTR regulation of subcellular localization may be more pervasive than currently known.