Figure 11.

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Recognition of familiar food activates feeding via an endocrine serotonin signal in Caenorhabditis elegans

Figure 11.

Figure 11.
Download figureOpen in new tabDownload powerpointFigure 11. Recognition of familiar food may increase serotonin release from ADF.

(A) Schematic of experimental design for anti-serotonin staining. Coding is as in Figure 1A. (BC) serotonin immunoreactivity in tph-1; Is[ptph-1::gfp]; Ex[ADF::tph-1(+)::gfp] (B) and in mod-5; tph-1; Is[ptph-1::gfp]; Ex[ADF::tph-1(+)::gfp], the paired control animals defective in serotonin uptake (C). The Is[ptph-1::gfp] allows the identification of NSM and ADF by GFP expression. Filled arrowheads and open arrowheads indicate ADFs and serotonin-uptaking cells, respectively. The serotonin signals not marked by arrowheads are neuronal processes. (D) Increase in the average number of serotonin-positive serotonin-uptaking cells during the 1 hr refeeding on familiar or novel food in tph-1; Is[ptph-1::gfp]; Ex[ADF::tph-1(+)::gfp] and in mod-5; tph-1; Is[ptph-1::gfp]; Ex[ADF::tph-1(+)::gfp] (see ‘Immunohistochemistry’, ‘Quantification of serotonin positive neurons’ and ‘Detailed data analysis’ in ‘Materials and methods’ for details). The baseline for each measurement is the average number of serotonin positive AIMs and RIH after starvation. The baseline was 2.06 ± 0.07 in animals trained on HB101 and 1.99 ± 0.05 in animals trained on DA1878. The number of animals examined (n = 3 independent assays per each group) is shown under each bar. Data shown as mean ± SEM, Student's t test (see ‘Detailed data analysis’ in ‘Materials and methods’).

DOI: http://dx.doi.org/10.7554/eLife.00329.013