1. Neuroscience

Multivariate analysis of electrophysiological diversity of Xenopus visual neurons during development and plasticity

  1. Christopher M Ciarleglio
  2. Arseny S Khakhalin
  3. Angelia F Wang
  4. Alexander C Constantino
  5. Sarah P Yip
  6. Carlos D Aizenman  Is a corresponding author
  1. Brown University, United States
https://doi.org/10.7554/eLife.11351
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  1. Christopher M Ciarleglio
  2. Arseny S Khakhalin
  3. Angelia F Wang
  4. Alexander C Constantino
  5. Sarah P Yip
  6. Carlos D Aizenman
(2015)
Multivariate analysis of electrophysiological diversity of Xenopus visual neurons during development and plasticity
eLife 4:e11351.
https://doi.org/10.7554/eLife.11351
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Abstract

Biophysical properties of neurons become increasingly diverse over development, but mechanisms underlying and constraining this diversity are not fully understood. Here we investigate electrophysiological characteristics of Xenopus tadpole midbrain neurons across development and during homeostatic plasticity induced by patterned visual stimulation. We show that in development tectal neuron properties not only change on average, but also become increasingly diverse. After sensory stimulation, both electrophysiological diversity and functional differentiation of cells are reduced. At the same time, the amount of cross-correlations between cell properties increase after patterned stimulation as a result of homeostatic plasticity. We show that tectal neurons with similar spiking profiles often have strikingly different electrophysiological properties, and demonstrate that changes in intrinsic excitability during development and in response to sensory stimulation are mediated by different underlying mechanisms. Overall, this analysis and the accompanying dataset provide a unique framework for further studies of network maturation in Xenopus tadpoles.

Article and author information

Author details

  1. Christopher M Ciarleglio

    Department of Neuroscience, Brown University, Providence, United States
    Competing interests
    The authors declare that no competing interests exist.

  2. Arseny S Khakhalin

    Department of Neuroscience, Brown University, Providence, United States
    Competing interests
    The authors declare that no competing interests exist.

  3. Angelia F Wang

    Department of Neuroscience, Brown University, Providence, United States
    Competing interests
    The authors declare that no competing interests exist.

  4. Alexander C Constantino

    Department of Neuroscience, Brown University, Providence, United States
    Competing interests
    The authors declare that no competing interests exist.

  5. Sarah P Yip

    Neuroscience, Brown University, Providence, United States
    Competing interests
    The authors declare that no competing interests exist.

  6. Carlos D Aizenman

    Department of Neuroscience, Brown University, Providence, United States
    For correspondence
    Carlos_Aizenman@brown.edu
    Competing interests
    The authors declare that no competing interests exist.

Reviewing Editor

  1. Mark CW van Rossum, University of Edinburgh, United Kingdom

Ethics

Animal experimentation: All handling of animals was approved by Brown University IACUC in accordance with NIH guidelines. The animal protocol used for these experiments is "Regulation of Neural Excitability and Synaptic Function by Experience in the Developing Visual System (#1308000008C002)."

Version history

  1. Received: September 3, 2015
  2. Accepted: November 12, 2015
  3. Accepted Manuscript published: November 14, 2015 (version 1)
  4. Version of Record published: December 16, 2015 (version 2)

Copyright

© 2015, Ciarleglio et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Christopher M Ciarleglio
  2. Arseny S Khakhalin
  3. Angelia F Wang
  4. Alexander C Constantino
  5. Sarah P Yip
  6. Carlos D Aizenman
(2015)
Multivariate analysis of electrophysiological diversity of Xenopus visual neurons during development and plasticity
eLife 4:e11351.
https://doi.org/10.7554/eLife.11351

Share this article

https://doi.org/10.7554/eLife.11351

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