A broadly distributed toxin family mediates contact-dependent antagonism between gram-positive bacteria
- McMaster University, Canada
- University of Washington, United States
- Newcastle University, United Kingdom
- Advanced Light Source, United States
- Northwestern University, United States
Abstract
The Firmicutes are a phylum of bacteria that dominate numerous polymicrobial habitats of importance to human health and industry. Although these communities are often densely colonized, a broadly distributed contact-dependent mechanism of interbacterial antagonism utilized by Firmicutes has not been elucidated. Here we show that proteins belonging to the LXG polymorphic toxin family present in Streptococcus intermedius mediate cell contact- and Esx secretion pathway-dependent growth inhibition of diverse Firmicute species. The structure of one such toxin revealed a previously unobserved protein fold that we demonstrate directs the degradation of a uniquely bacterial molecule required for cell wall biosynthesis, lipid II. Consistent with our functional data linking LXG toxins to interbacterial interactions in S. intermedius, we show that LXG genes are prevalent in the human gut microbiome, a polymicrobial community dominated by Firmicutes. We speculate that interbacterial antagonism mediated by LXG toxins plays a critical role in shaping Firmicute-rich bacterial communities.
Article and author information
Author details
Funding
Canadian Institutes of Health Research
- John C Whitney
Natural Sciences and Engineering Research Council of Canada
- Adrian J Verster
Wellcome (101824/Z/13/Z)
- Waldemar Vollmer
National Cancer Institute (CCSG P30 CA060553)
- Young Ah Goo
National Institutes of Health (AI080609)
- Joseph D Mougous
Howard Hughes Medical Institute
- Joseph D Mougous
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Michael T Laub, Massachusetts Institute of Technology, United States
Version history
- Received: March 20, 2017
- Accepted: July 10, 2017
- Accepted Manuscript published: July 11, 2017 (version 1)
- Accepted Manuscript updated: July 12, 2017 (version 2)
- Version of Record published: August 14, 2017 (version 3)
- Version of Record updated: August 24, 2017 (version 4)
Copyright
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
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A broadly distributed toxin family mediates contact-dependent antagonism between gram-positive bacteria
eLife 6:e26938.
https://doi.org/10.7554/eLife.26938
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