Fast retrieval and autonomous regulation of single spontaneously recycling synaptic vesicles
Abstract
Presynaptic terminals release neurotransmitters spontaneously in a manner that can be regulated by Ca2+. However, the mechanisms underlying this regulation are poorly understood because the inherent stochasticity and low probability of spontaneous fusion events has curtailed their visualization at individual release sites. Here, using pH-sensitive optical probes targeted to synaptic vesicles, we visualized single spontaneous fusion events and found that they are retrieved extremely rapidly with faster re-acidification kinetics than their action potential-evoked counterparts. These fusion events were coupled to postsynaptic NMDA receptor-driven Ca2+ signals, and at elevated Ca2+ concentrations there was an increase in the number of vesicles that would undergo fusion. Furthermore, spontaneous vesicle fusion propensity in a synapse was Ca2+-dependent but regulated autonomously: independent of evoked fusion probability at the same synapse. Taken together, these results expand classical quantal analysis to incorporate endocytic and exocytic phases of single fusion events and uncover autonomous regulation of spontaneous fusion.
Article and author information
Author details
Reviewing Editor
- Christian Rosenmund, Charité-Universitätsmedizin Berlin, Germany
Ethics
Animal experimentation: All animal protocols were approved by the Institutional Care and Use Committee at UT Southwestern Medical Center. The work presented in this study is covered by the Animal Protocol Numbers APN 0866-06-05-1 and APN 0866-06-03-1
Version history
- Received: June 11, 2014
- Accepted: November 21, 2014
- Accepted Manuscript published: November 21, 2014 (version 1)
- Version of Record published: December 15, 2014 (version 2)
Copyright
© 2014, Leitz & Kavalali
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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