Tools and Resources

A Tools and Resources article allows authors to publish the details of new experimental techniques, datasets, software tools, and other resources.

Latest articles

    1. Ecology

    Full factorial construction of synthetic microbial communities

    Juan Diaz-Colunga, Pablo Catalan ... Alvaro Sanchez
    A rapid, inexpensive, and easy to implement experimental protocol enables the construction of combinatorially complete sets of microbial consortia.
    1. Immunology and Inflammation

    Cas9+ conditionally immortalized neutrophil progenitors as a tool for genome wide CRISPR screening for neutrophil differentiation and function

    Robyn M Jong, Krystal L Ching ... Sarah A Stanley
    1. Neuroscience

    Functional imaging of nine distinct neuronal populations under a miniscope in freely behaving animals

    Mary L Phillips, Nicolai T Urban ... Ryohei Yasuda
    Multiplexed spectral fingerprinting through a single implanted GRIN lens enables projection-resolved functional imaging of up to nine neuronal populations in freely behaving animals without post hoc histology.
    1. Chromosomes and Gene Expression
    2. Computational and Systems Biology

    Real-time transcriptomic profiling in distinct experimental conditions

    Tamer Butto, Stefan Pastore ... Susanne Gerber
    Real-time nanopore transcriptomics enables early detection of expression changes and sample quality, offering a rapid, cost-effective strategy for experimental quality control and transcriptomic analysis.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Interrogating the structure and function of the human voltage-gated proton channel (hHv1) with a fluorescent noncanonical amino acid

    Emerson M Carmona, William N Zagotta, Sharona E Gordon
    A fluorescent noncanonical amino acid enables structural measurements across full-length human voltage-gated proton channel (Hv1) and reveals zinc-dependent conformational changes.
    1. Structural Biology and Molecular Biophysics

    PPIscreenML is a method for structure-based screening of protein-protein interactions using AlphaFold

    Victoria Mischley, Johannes Maier ... John Karanicolas
    PPIscreenML is a rigorously benchmarked method that uses AlphaFold2 to screen for interacting protein pairs, and it provides superior performance to other available methods.
    1. Developmental Biology
    2. Genetics and Genomics

    The robust, high-throughput, and temporally regulated roxCre and loxCre reporting systems for genetic modifications in vivo

    Mengyang Shi, Jie Li ... Bin Zhou
    New mouse genetic tools, roxCre and loxCre, facilitate the study of gene function in cells of interest, allowing for simultaneous and efficient gene knockout and precise lineage tracing in vivo.
    1. Ecology
    2. Neuroscience

    New idtracker.ai rethinks multi-animal tracking as a representation learning problem to increase accuracy and reduce tracking time

    Jordi Torrents, Tiago Costa, Gonzalo de Polavieja
    Identification of each animal in a collective becomes possible even when individuals are never all visible simultaneously, enabling faster and more accurate analysis of collective behavior.
    1. Cell Biology

    Probing metazoan polyphosphate biology using Drosophila reveals novel and conserved polyP functions

    Sunayana Sarkar, Harsha Sharma ... Manish Jaiswal
    A genetic toolkit establishes Drosophila as a tractable metazoan system for systematic interrogation of conserved biological functions of the ancient polymer polyphosphate.
    1. Chromosomes and Gene Expression
    2. Computational and Systems Biology

    DNA O-MAP uncovers the molecular neighborhoods associated with specific genomic loci

    Yuzhen Liu, Christopher D McGann ... Devin K Schweppe
    DNA O-MAP enables proximity labeling at specific genomic loci in fixed cells using programmable oligonucleotides, revealing locus-proximal proteomes and chromatin interactions without genetic modification.
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