ADPT enhances the robustness of animal pose estimation by minimizing keypoint tracking drift, enabling more accurate multi-animal tracking and facilitating refined behavioral analysis across species.
Novel genetically encoded tools PFE3, paGFE3, and chGFE3 can be used to reversibly ablate excitatory synapses constitutively, and inhibitory synapses in a light- or chemically dependent manner.
SAVEMONEY is a computational tool that guides users to mix plasmids prior to nanopore sequencing, reducing the effective cost of whole-plasmid sequencing to below that of Sanger sequencing.
The established under-flow migration tracker (UFMTrack) framework allows for automated and scalable studies of immune cell interactions with and extravasation across endothelial monolayer under flow in vitro.
Eric R Brooks, Andrew R Moorman ... Jennifer A Zallen
Single-cell RNA sequencing and computational analysis define a spatial and temporal map of gene expression during early patterning and morphogenesis of the mouse cranial neural plate, providing a resource for elucidating the transcriptional basis of mammalian brain development.
Jianheng Fox Liu, Ben R Hawley ... Samie R Jaffrey
CROWN-seq provides the first precise and quantitative map of N6,2’-O-dimethyladenosine (m6Am) in mRNA and snRNA, revealing the link between m6Am modification and efficient transcription, rather than translation and RNA stability.
Jasper Janssens, Pierre Mangeol ... Frank Schnorrer
Spatial transcriptomics of adult Drosophila brain and body sections determined the spatial expression for 150 genes, which identified the locations of unknown neuronal cell types and transcriptional diversity within muscle.
Gabriel Loewinger, Erjia Cui ... Francisco Pereira
A fiber photometry analysis framework based on functional mixed models enhances the detection of effects by testing signal-variable associations at each trial timepoint and accounting for between-animal heterogeneity.
Barbora Rehak Buckova, Charlotte Fraza ... Jaroslav Hlinka
Normative modelling adapted for longitudinal neuroimaging enables precise evaluation of individual brain changes over time, revealing subtle disease dynamics and temporal patterns missed by traditional analytical approaches.
