Victoria Mischley, Johannes Maier ... John Karanicolas
PPIscreenML is a rigorously benchmarked method that uses AlphaFold2 to screen for interacting protein pairs, and it provides superior performance to other available methods.
New mouse genetic tools, roxCre and loxCre, facilitate the study of gene function in cells of interest, allowing for simultaneous and efficient gene knockout and precise lineage tracing in vivo.
Identification of each animal in a collective becomes possible even when individuals are never all visible simultaneously, enabling faster and more accurate analysis of collective behavior.
A genetic toolkit establishes Drosophila as a tractable metazoan system for systematic interrogation of conserved biological functions of the ancient polymer polyphosphate.
Yuzhen Liu, Christopher D McGann ... Devin K Schweppe
DNA O-MAP enables proximity labeling at specific genomic loci in fixed cells using programmable oligonucleotides, revealing locus-proximal proteomes and chromatin interactions without genetic modification.
Vitaly Ryu, Anisa Azatovna Gumerova ... Mone Zaidi
The compendium of oxytocin and its receptor in the brain could provide further insights into the peripheral and central effects of oxytocin in health and disease.
Isobel Parkes, Ara Schorscher-Petcu ... Liam E Browne
A closed-loop system delivers cutaneous optical stimulation during movement in freely behaving mice, enabling controlled somatosensory input in naturalistic settings.
A comprehensive toolkit enables genome-wide, recombinase-independent mosaic analysis in Drosophila, permitting clonal analysis of pericentromeric genes, deficiency chromosomes, and interspecific hybrids previously inaccessible to standard methods.
New synaptically targeted GCaMP8 sensors and analysis tools capture calcium dynamics with the speed and sensitivity previously achievable only through electrophysiology and chemical dyes.
