To identify neurons, which require dFz2 function for flight, an RNAi strain (dFz2-IR) was expressed in independent neurotransmitter domains with the help of the UASGAL4 system for cell and tissue specific expression (Brand and Perrimon, 1993). Amongst the neuronal domains tested, significant flight deficits were observed upon knockdown of dFz2 in aminergic neurons (60% flight time; DdcGAL4) and in dopaminergic neurons (45% flight time; THGAL4) (Figure 1A, Video 1). Because, DdcGAL4 drives expression in serotonergic and dopaminergic neurons, we tested flies with knockdown of dFz2 in serotonergic neurons (TRHGAL4). These flies exhibit normal flight bouts in the tethered flight assay (Figure 1A). Similarly, normal flight bouts were observed in flies with knockdown of dFz2 by OK371GAL4 (mainly glutamatergic neurons), GADGAL4 (many GABA-ergic neurons), and P386GAL4 (a peptidergic neuron subset that expresses in cells with the neuropeptide processing enzyme amontillado; Figure 1—figure supplement 1). Thus, dFz2 function is required primarily in flight circuit neurons, which express GAL4 under control of the pale (ple) gene promoter that codes for TH. TH catalyzes a rate-limiting enzymatic step in the synthesis of dopamine (Friggi-Grelin et al., 2003). Therefore, expression of this enzyme is considered a reliable marker of dopaminergic neurons. The THGAL4 strain marks a high proportion of TH-positive neurons in the Drosophila central nervous system (Friggi-Grelin et al., 2003).

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An earlier study demonstrated that pan-neuronal knockdown of dFz2 in pupae leads to flight deficits. However, dFz2 knockdown in adults did not affect flight (Agrawal et al., 2013). Existing larval progenitor neurons undergo extensive re-modeling of their axonal and dendritic arbors during pupal stages to form synaptic connections of the mature adult flight circuit (Fernandes and VijayRaghavan, 1993; Consoulas et al., 2002). We therefore determined the developmental stage at which dFz2 function is required in dopaminergic neurons for flight. For this purpose, we used the TARGET (temporal and regional gene expression targeting) system (McGuire et al., 2003). TARGET regulates GAL4 expression by a temperature sensitive GAL80^ts element, which can be expressed and repressed at 18°C and 30°C, respectively (McGuire et al., 2004). Experimental animals of the genotype THGAL4,GAL80^ts;dFz2-IR were shifted to the permissive GAL4 expression temperature (30°C) either during pupal or adult stages. This allowed stage-specific knockdown of dFz2. Upon knockdown of dFz2 in TH-expressing neurons through pupal development a significant reduction of flight time was observed, whereas normal flight bouts, as measured for 30 s, were observed upon knockdown of dFz2 in adults (Figure 1B). Flight deficits upon knockdown of dFz2 in pupae were equivalent to those observed upon knockdown throughout post-embryonic development confirming that dFz2 requirement for flight is primarily in pupal dopaminergic neurons during circuit maturation. A physiological correlate of flight is rhythmic patterns of action potentials recorded from the dorsal longitudinal muscles (DLMs) during tethered flight. A reduction in duration of flight patterns was observed upon knockdown of dFz2 in TH-expressing neurons during pupal development (Figure 1C,D). dFz2 knockdown at pupal and adult stages in TH-expressing neurons was confirmed by qPCR. As a control, we also confirmed dFz2 knockdown in serotonergic neurons targeted by TRHGAL4 where no flight deficits were observed (Figure 1E). The specificity of flight deficits obtained upon dFz2 knockdown was tested by expression of three additional RNAi strains for dFz2 (BL27568, BL31390, and BL31312). Significant flight defects ranging from 67% to 72% were obtained upon knockdown through post-embryonic development (Figure 1—figure supplement 2). The difference in flight deficits between dFz2-IR and the three other RNAi strains is very likely due to a difference in their efficacy of knockdown (compare Figure 1E with Figure 1—figure supplement 2B). Therefore, the dFz2-IR strain was used for all subsequent analyses.

Dopaminergic neurons marked by THGAL4 have been broadly classified into seven clusters in the brain (Figure 2A; Table 1). In addition TH-expressing neurons are present in each segment of the ventral ganglion (Mao and Davis, 2009; Sadaf et al., 2015). In the brain, two neuronal clusters referred to as PAM and PAL (Protocerebral Anterior Lateral) are located in the anterior region, whereas five neuronal clusters, PPM1, 2, and 3 (Protocerebral Posterior Medial), PPL1 and 2 (Protocerebral Posterior Lateral) are located in the posterior region (Figure 2A). In order to identify TH-expressing neurons that require dFz2 function for flight, three independent GAL4 strains (THC’, THC1, and THF2; Liu et al., 2012), with differential expression in central brain clusters and the ventral ganglion, were tested (Table 1). Significant flight deficits were observed upon expression of dFz2-IR under control of THC1GAL4, but not with THC’GAL4 and THF2GAL4 (Figure 2B). These data suggested that either all or some neurons in the PAM, PPM1, and T3 regions, marked by THC1GAL4, but poorly marked or not marked by THC’GAL4 and THF2GAL4, form part of the flight circuit and require dFz2 signaling during pupal development. Next, we tested two strains (NP6510GAL4 and R58E02GAL4) (Riemensperger et al., 2013) which drive expression uniquely in the PAM neurons (Table 1). Significant flight deficits were observed in flies with knockdown of dFz2 by either NP6510GAL4 or R58E02GAL4 (Figure 2B,C, Figure 2—figure supplement 1, Video 2) implicating these dopaminergic neurons as part of a central brain flight circuit. These data do not rule out a role for additional central neurons or ventral ganglion neurons in the regulation of flight. Expression of dFz2 was confirmed in adult PAM neurons by immunohistochemistry (Figure 2—figure supplement 2). Knockdown by dFz2-IR in PAM neurons resulted in significant loss of dFz2 immunostaining (Figure 2—figure supplement 2). Moreover, in support of the pupal requirement for dFz2 (Agrawal et al., 2013) (Figure 1B), PAM neurons marked by R58E02GAL4 do not express TH in the larval stages (Figure 2—figure supplement 3), indicating that TH immunoreactivity in these neurons is acquired during pupal maturation, as observed by co-localization of TH immunostaining with R58E02GAL4-driven GFP in pupae (Figure 2—figure supplement 3). With this we concluded that PAM neurons require dFz2 signaling during functional maturation of the flight circuit in pupae.

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A role for dFz2 in flight circuit development was originally identified in a screen for GPCRs that signal through changes in intracellular Ca²⁺ (Agrawal et al., 2013). In order to identify molecules that function downstream of Fz2 for development of the adult flight circuit, interactions of candidate genes were tested. Reports from vertebrates suggest that dFz2 activates downstream Ca²⁺ signaling through non-canonical mechanisms (Slusarski et al., 1997). We tested flight in animals with dFz2 knockdown in combination with both canonical (Figure 3—figure supplement 1) and non-canonical (Figure 3A) candidate genes. In the canonical signaling pathway dFz2 along with its co-receptor lipoprotein receptor related-protein 5/6 (LRP5/6, encoded by Arrow in Drosophila) activates Dishevelled. Activated dishevelled functions to stabilize β catenin (Drosophila armadillo) and hence promote β catenin entry into the nucleus followed by enhanced transcription of downstream target genes. We over-expressed wild-type Dishevelled, a point mutant (G64V) in the DIX-domain of Dishevelled (specifically activates the canonical pathway; Penton et al., 2002) and a constitutively active form of Armadillo (UAS-armS10; Morel and Arias, 2004) in the background of dFz2 down-regulation either in dopaminergic neurons or across all neurons. The resultant progeny were tested for flight. Up-regulation of canonical signaling molecules did not rescue flight deficits of dFz2 down-regulation (Figure 3—figure supplement 1). Moreover, normal flight times were observed in flies with RNAi knockdown of canonical dFz2 pathway components like LRP5/6, Dishevelled, and GSK3β in either the pan-neuronal domain or in dopaminergic neurons (Figure 3—figure supplement 1). RNAi strains for dishevelled and GSK3β were validated by quantitative PCR (qPCR; Figure 3—figure supplement 1), whereas RNAi for LRP5/6 was validated by ubiquitous expression with Act5CGAL4 that resulted in embryonic lethality (Dietzl et al., 2007). These results do not support a role for canonical dFz2 signaling in dopaminergic neurons for maturation of the flight circuit in pupae.

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Next, we tested flight deficits by expression of previously implicated non-canonical candidates that link dFz2 activation with Ca²⁺ signaling (Figure 3A) (Sheldahl et al., 2003). From genetic studies, we know that the heterotrimeric G-protein, Gq, which links GPCR activation to intracellular store calcium release, does not function downstream of dFz2 signaling in the context of Drosophila flight (Agrawal et al., 2013). Therefore, we tested the requirement of other heterotrimeric G-proteins from Drosophila. Constitutively, active forms of the α subunits of Gs (UASAcGs), Go (UASAcGo), and Gi (UASAcGi) were tested in flies with dFz2 knockdown in pupal stages. Pan-neuronal expression of AcGo in pupae with dFz2 knockdown, rescued flight defects to a significant extent (Figure 3B), whereas constitutively active forms of Gi, Gq, or Gs did not (Figure 3—figure supplement 2). A partial rescue of flight defects was also observed upon AcGo expression in dopaminergic neurons in pupae (Figure 3B). These data support Go activation by dFz2 in dopaminergic neurons of the maturing flight circuit during pupal development.

To confirm the requirement of Go in dopaminergic neurons, we down-regulated Go function either by expression of an RNAi construct (Vecsey et al., 2014) THGAL4;GoRNAi or by expression of pertussis toxin which inhibits Go function (THGAL4;UASPTX.16) and tested the progeny for flight. In Drosophila, pertussis toxin is a selective inhibitor of Go signaling (Hopkins et al., 1988; Ferris et al., 2006). Expression of Go-IR and PTX.16 in dopaminergic neurons reduced both flight times (Figure 3B), and the maintenance time of flight patterns recorded from the DLMs (Figure 3D,E). Moreover, down-regulation of Go signaling in PAM neurons with R58E02GAL4, by expression of either Go-IR or PTX.16 resulted in significant loss of flight (Figure 3B). PTX induced flight deficits required expression in pupae and not in adults (Figure 3B and Figure 3—figure supplement 2).

Flight deficits induced by pan-neuronal knockdown of dFz2 were rescued to a significant extent by over-expression of the ER Ca²⁺ depletion sensor, dSTIM⁺ (Agrawal et al., 2013), suggesting that activation of Go by dFz2 evokes Ca²⁺ signals in Drosophila neurons. As in other organisms (Feske et al., 2006; Prakriya et al., 2006; Vig et al., 2006), in Drosophila neurons as well Ca²⁺ release through the IP₃R leads to clustering of dSTIM, which in turn promotes Store-operated Ca²⁺ entry (SOCE) through dOrai (Venkiteswaran and Hasan, 2009; Agrawal et al., 2010) (Figure 3A). In a converse experiment, we tested the effect of over-expression of a dFz2⁺ transgene on flight deficits induced by pan-neuronal knockdown of the IP₃R (itpr-IR), dSTIM (dSTIM-IR) and dOrai (dOrai-IR) and observed a significant rescue in all three conditions tested (Figure 3—figure supplement 3, Video 3, and Video 4). These data suggest that, as in vertebrate neurons, dFz2 links to intracellular calcium signaling in Drosophila. Next, we tested the effect of over-expression of dSTIM⁺, on flight deficits induced by dFz2 knockdown in dopaminergic neurons. A partial but significant rescue of flight was observed (Figure 3C) accompanied by a rescue of the duration of firing patterns from the DLMs (Figure 3D,E). Over-expression of the IP₃R and dOrai also rescued flight deficits observed by knockdown of dFz2 in dopaminergic neurons (Figure 3C,D,E). Together these data support the idea that maturation of dopaminergic neurons in the flight circuit requires intracellular Ca²⁺ signaling by activation of dFz2 and Go. The mechanism by which Go activates Ca²⁺ signaling through IP₃R/dSTIM requires further investigation (Figure 3A).

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Synaptic function of developing hippocampal neurons can be modulated by Ca²⁺ signaling downstream of Fz2 (Varela-Nallar et al., 2010). In Drosophila, neuronal activity can be increased by over-expression of a voltage-gated sodium channel, NaChBac (Nitabach et al., 2006). Therefore, we tested flight in organisms with dFz2 knockdown and increased neuronal activity by NaChBac expression. Flight was restored close to 100% upon expression of NaChBac in dopaminergic neurons (THGAL4) and more specifically in PAM neurons (NP6510GAL4 and R58E02GAL4; Figure 4A, Video 5). Moreover, raising neuronal activity during pupal development in parallel with dFz2 knockdown compensated for loss of flight observed in the knockdown condition (Figure 4B). These data suggest that Fz2/Ca²⁺ signaling can contribute to the synaptic activity of dopaminergic PAM neurons in pupae. The requirement for synaptic activity in maturing PAM neurons was tested directly by expression of a temperature sensitive mutant of the dynamin orthologue, shibire^ts (Shi^ts). Expression of Shi^ts blocks vesicle endocytosis at 30°C (Kitamoto, 2001) and its expression during pupal development, either in TH neurons (THGAL4) or exclusively in PAM neurons (NP6510GAL4 and R58E02GAL4) resulted in significant loss of flight (Figure 4C). Temporal expression of Shi^ts in adult PAM neurons also resulted in a flight deficit (Figure 4D), supporting the requirement of active synaptic transmission in PAM neurons for adult flight.

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The cellular effect of reduced dFz2 expression in PAM neurons was investigated next. TH levels in PAM neurons marked by R58E02GAL4, appear reduced upon dFz2 knockdown as judged by immunohistochemistry (compare anti-TH panels in Figure 5A,B). Expression of NaChBac with dFz2-IR restored TH expression close to wild-type levels (Figure 5A,B,C). Quantification of TH immunostaining across multiple samples revealed a significant reduction upon dFz2 knockdown which was restored by expression of NaChBac (Figure 5D,E). Furthermore, TH transcript levels were significantly reduced by dFz2 knockdown and were restored upon expression of NaChBac (Figure 5—figure supplement 1). Thus, altered TH levels corroborated well with flight deficits and their rescue in various genotypes. Numbers of TH-positive neurons in the PAM cluster were not significantly different between the three genotypes as judged by anti-TH immunostaining (Figure 5G). Surprisingly, R58E02GAL4-driven GFP expression was also reduced upon expression of dFz2-IR and was restored back upon expression of NaChBac (Figure 5—figure supplement 1). Consequently, there was an apparent reduction in the numbers of GFP positive cells upon dFz2-IR expression which was restored by NaChBac expression (Figure 5F). Because the number of TH-expressing cells of the PAM cluster remained unchanged upon dFz2 knockdown and after NaChBac rescue (Figure 5G), we hypothesized that dFz2/Ca²⁺ signaling regulates TH expression in PAM neurons during pupal development. Moreover, our data support a role for dFz2/Ca²⁺ signaling in regulating expression of the R58E02GAL4 transgene where GAL4 is under control of the fumin gene encoding a Dopamine Transporter, DAT (Liu et al., 2012).

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The status of TH and GFP expression in PAM neurons was further investigated after dFz2 knockdown with THGAL4. Unlike R58E02GAL4, THGAL4-driven mGFP marks a small subset of PAM neurons. This consists of two clusters of 6–7 neurons each (Figure 2A and Figure 5—figure supplement 2) (Riemensperger et al., 2013). THGAL4-driven expression of dFz2-IR resulted in significant loss of GFP expression in PAM neurons (Figure 5—figure supplement 2). Interestingly, GFP expression in five TH-positive neurons of the PAL cluster remained unaffected by knockdown of dFz2, suggesting that dFz2 regulation of TH expression maybe PAM specific (data not shown). Over-expression of either dSTIM⁺, IP₃R or NaChBac in the background of dFz2 knockdown could partially rescue loss of GFP expression in the PAM neurons (Figure 5—figure supplement 2). Thus, THGAL4-driven GFP expression in PAM neurons correlated with flight deficits and their rescue (Figures 1A, 3C, 4A). Quantification of TH immunoreactivity in PAM cells by THGAL4-driven dFz2 knockdown was technically not possible because the few THGAL4-positive cells of the PAM cluster could not be identified in the dFz2 knockdown condition. Taken together these data support the idea that dFz2/Ca²⁺ signaling in PAM neurons drives transcription of two key dopamine synthesis and uptake molecules, TH and DAT. The transcriptional regulation extends to GAL4 transgenic constructs containing TH and DAT regulatory sequences. As controls we tested TH immunoreactivity of PAM neurons in flies with dFz2 knockdown in glutamatergic neurons (OK371GAL4). Both TH immunoreactivity of PAM neurons (Figure 5—figure supplement 3) and flight patterns (Figure 1—figure supplement 1) were similar to controls.

Based on our observation that dFz2 knockdown in PAM neurons leads to flight deficits accompanied by a significant reduction of TH expression, we tested the requirement of TH in PAM neurons for flight. Over-expression of a neuronal-specific TH cDNA transgene (UASDTH1) (Friggi-Grelin et al., 2003) in flies with dFz2 knockdown by PAMGAL4 strains (NP6510GAL4 and R58E02GAL4) could rescue flight deficits significantly (Figure 6A, Video 6, Figure 6—figure supplement 1). Furthermore, knockdown of TH with an RNAi (dTH-IR) resulted in significant loss of flight and reduced TH expression (Figure 6A–C,F, Figure 6—figure supplement 1). Moreover, knockdown of TH in PAM neurons affected R58E02GAL4-driven GFP expression suggesting feedback regulation of dopamine transporter (DAT) by dopamine levels. Over-expression of the DTH1 neuronal cDNA could rescue TH immunoreactivity in the R58E02GAL4-expressing PAM neurons with dFz2 knockdown (Figure 6B,C,E). However, GFP immunoreactivity remained low and unchanged between dFz2-IR- and dFz2-IR;DTH1-expressing PAM neurons (Figure 6—figure supplement 1). These data suggest that rescue of flight by over-expression of DTH1 by passes the transcriptional regulation of DAT by dopamine and of endogenous TH by dFz2/Ca²⁺ signaling. They confirm the requirement for TH expression in PAM neurons for flight.

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PAM neurons send projections to the horizontal lobes of the MB neuropil (Aso et al., 2012; Burke et al., 2012; Liu et al., 2012; Riemensperger et al., 2013). The MB is a paired brain structure that controls several higher brain functions in insects ranging from olfactory memory formation and reinforcement (Kahsai and Zars, 2011; Waddell, 2013) to locomotor activity (Helfrich-Forster et al., 2002; Serway et al., 2009; Riemensperger et al., 2013). Axons and dendrites of Kenyon cells, positioned in the calyx region form the MB neuropil which is subdivided into the α, β, α′, β′, and γ lobes (Strausfeld et al., 2003). We reasoned that flight deficits observed due to reduced levels of TH in PAM neurons might derive from reduced dopamine release and signaling in postsynaptic MB neurons. This idea was tested by silencing specific MB neuropil lobes with mb186bGAL4 and mb247GAL4 drivers. mb186bGAL4 is a recently generated split GAL4 strain (Aso et al., 2014) whose expression is restricted to the α′ β′ lobes (Vogt et al., 2014), whereas mb247GAL4 is expressed in the α, β, and γ lobes (Zars, 2000; Krashes et al., 2007; Pech et al., 2013). Synaptic release in MB neurons of adult flies was inhibited by expression of a temperature sensitive dominant negative dynamin transgene (UAS-Shi^ts) under control of either mb186bGAL4 or mb247GAL4 drivers. Blocking synaptic release in α′, β′ lobes resulted in a strong flight deficit, whereas silencing of the α, β , and γ lobes did not have a significant effect on flight, for 30 s (Figure 7A). Reduced flight bouts were accompanied by loss of rhythmic spiking in the DLMs of flies with silenced α′, β′ lobes neurons (Figure 7B). These data support a requirement for post-synaptic dopamine receptors in MB neurons that function for maintenance of acute flight. We tested this requirement further by RNAi-mediated knockdown of the four dopamine receptors—DopECR (CG18314), Dop1R1 (CG9652), Dop1R2 (CG18741), and Dop2R (CG33517), in either the α′, β′ neurons (mb186bGAL4) or the α, β, γ neurons (mb247GAL4; Figure 7—figure supplement 1). A reduction in the length of flight bouts was observed specifically upon knockdown of Dop1R2 in the α′, β ′ neurons (Figure 7A). The role of α′ β′ lobes, in flight was supported by another GAL4 driver, c305GAL4, which expresses in the α′ β′ lobes and faintly in the γ lobe (Krashes et al., 2007; Pech et al., 2013). Blocking synaptic activity or knockdown of Dop1R2 using c305aGAL4 resulted in significant flight deficits (Figure 7—figure supplement 2).

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Unlike dopaminergic neurons located in the ventral ganglion, which directly modulate flight motor neuron function as demonstrated recently (Sadaf et al., 2015), the PAM–MB circuit described here is not known to project to flight motor neurons in the ventral ganglion (Riemensperger et al., 2013). Rather, PAM-MB circuits function to reinforce both aversive and appetitive olfactory responses (Waddell, 2013). To test possible re-inforcement of flight time by the PAM-MB circuit, we monitored longer flight bouts in several genotypes. Knockdown of dFz2 in PAM neurons (R58E02GAL4>dFz2-IR) significantly reduced the duration of flight bouts, monitored up to 15 min, from an average of 13.04 ± 0.4 min in controls to 0.85 ± 0.3 min in the knockdowns (Figure 7C). Maintenance of flight bouts was rescued significantly upon increasing neuronal activity by expression of NaChBac (Figure 7C). These data were analyzed further by binning flight bouts in 20-s intervals (Figure 7D). Flight time of dFz2 knockdown flies clustered towards the left among shorter flight bouts, whereas control flies clustered towards the right with longer flight bouts (Figure 7D). Distribution of flight times in NaChBac-rescued flies appeared intermediate. All rescued flies flew for longer than 20 s and a small percentage flew for longer than 10 min. Interestingly, DTH1-rescued flies exhibit shorter flight bouts as compared with NaChBac-rescued organisms (Figure 7C,D). This difference in rescue abilities may in part be due to the previous observation that NaChBac rescue restores TH immunoreactivity to PAM neurons (Figure 5C), whereas TH rescue very likely cannot restore the excitability deficit of PAM neurons. Long flight bouts were also tested in flies with dFz2 knockdown by THGAL4, followed by rescue with dSTIM⁺, itpr⁺, and NaChBac (Figure 7—figure supplement 4). The NaChBac rescue profile was very similar to that observed by NaChBac rescue of PAM-specific dFz2 knockdown, whereas, dSTIM⁺ and itpr⁺ rescue profiles resembled the DTH1 rescue in Figure 7C,D (Figure 7—figure supplement 5). These data support a role for the PAM-MB circuit in maintenance of long flight bouts through dopaminergic synapses on α' β' MB lobes.

Differentiation of neuronal subtypes, after genetic specification, is subject to multiple signals many of which generate and modify electrical activity of the cognate neurons (Borodinsky et al., 2014). We demonstrate a requirement for dFz2/Ca²⁺ signaling for maintaining TH levels in a subset of central brain dopaminergic neurons—the PAM cluster. Our results support transcriptional regulation of TH and very likely the dopamine transporter (DAT) by dFz2/Ca²⁺ signaling in the PAM neurons. A significant compensation of the flight deficit was observed in flies with dFz2 knockdown in PAM neurons upon over-expression of the sodium channel NaChBac, indicating that dFz2/Ca²⁺ signaling also affects neural activity of PAM neurons. Moreover, flight deficits were observed upon expression of Shi^ts in PAM neurons during the pupal stages, supporting a role for neural activity and synaptic transmission in their development. Increased TH transcripts and TH immunoreactivity after rescue by NaChBac suggests that dFz2/Ca²⁺ signaling can in part be compensated by raised neural activity, and possibly the two signaling mechanisms function in parallel for maintaining TH transcription in PAM neurons. It is likely that in addition to TH and DAT, dFz2/Ca²⁺ signals exert their influence on other transcripts in PAM neurons. Transcriptional profiling of the PAM neurons is necessary to address this possibility. Interestingly, despite an increase in the number of PAM neurons during pupal maturation (Figure 2—figure supplement 3), we do not observe an affect of dFz2/Ca²⁺ signaling on the number of PAM neurons. Knockdown of dFz2 by DdcGAL4 that marks >60 PAM neurons resulted in flight time of ∼65% (Figure 1A), whereas a stronger flight deficit was observed with NP6510GAL4 which marks just 15 PAM neurons (Figure 2B). Moreover, the flight deficit obtained with R58E02GAL4 which marks ∼100 PAM neurons was similar to the flight deficit obtained with NP6510GAL4 (Figure 2B). Thus, the numbers of PAM neurons that express dFz2-IR do not correlate with the extent of observed flight deficits, suggesting that flight is regulated by a subset of PAM neurons and their projections to the MB. Further, analysis with GAL4 strains that mark PAM neuronal sub-domains would be helpful in identifying such flight specific PAM neurons.

Down-regulation of GFP fluorescence in PAMGAL4 strains upon dFz2 knockdown prevented direct analysis of their projections to the MB. Based on a recent study demonstrating similar PAM-MB connections for negative geotaxis (Riemensperger et al., 2013), we measured climbing in PAM > dFz2-IR flies. This appeared similar to controls (Figure 7—figure supplement 3), supporting the idea that connections of PAM neurons to the MB are maintained upon dFz2 knockdown. Thus as compared to climbing, flight appears more sensitive to the observed imbalance of TH. However, as expected inhibition of synaptic release from PAM neurons (R58E02GAL4>Shi^ts; at 30°C) affected both flight (Figure 4D) and climbing (Figure 7—figure supplement 3).

In vertebrates, βγ subunits of the trimeric G_o protein activate phospholipase Cβ, which in turn enhances IP₃ formation followed by IP₃ receptor-mediated release of calcium from endoplasmic reticular stores (Rebecchi and Pentyala, 2000). In Xenopus embryos, non-canonical Wnt/Ca²⁺ signaling, acting through Fz receptors, activates the Nuclear Factor of Activated T cells (NFAT) which regulates transcription of genes required for dorsoventral axis formation (Saneyoshi et al., 2002). Apart from NFAT, non-canonical Fz2/Ca²⁺ signaling can also activate calcium calmodulin-dependent protein kinase II (CamKII) and protein kinase C (PKC) which regulate activity of transcription factors, such as NFκB and CREB (Sheldahl et al., 1999; Kuhl et al., 2000; Slusarski and Pelegri, 2007). Non-canonical dFz2/Ca²⁺ signaling has been poorly characterized in Drosophila. dFz2 can be cleaved and imported into the nucleus in Drosophila neurons (Mosca and Schwarz, 2010). However, we do not favor direct transcriptional control of TH by cleaved dFz2 for the following reasons. Rescue of flight in dFz2 knockdown flies can be achieved by AcGo, itpr⁺, and dSTIM⁺ (Figure 2). These data support a link between dFz2 activation of Go at the membrane followed by intracellular Ca²⁺ release through the IP₃R and dSTIM-mediated calcium entry. This mechanism is broadly similar to what has been observed in vertebrates. Moreover, we did not detect nuclear dFz2 in PAM neurons (Figure 2—figure supplement 2).

Our observation that knockdown of dFz2 reduced not only transcripts from the endogenous TH gene but also affected GFP expression from a DAT promoter transgene (R58E02GAL4>GFP) suggests co-ordinated transcriptional regulation of genes for maintenance of dopamine levels by Fz2/Ca²⁺ signaling in PAM neurons. However, the molecular mechanism by which reduced Fz2/Ca²⁺ signaling regulates transcription of TH and very likely other genes, in PAM neurons remains to be elucidated.

Insect MBs are lobed structures located bilaterally in the protocerebrum of the central nervous system. Neuro-anatomical studies have demonstrated the presence of both efferent neurons arising from MB lobes as well as afferent connections supplying the MB lobes from protocerebral regions (Ito et al., 1998) including dopaminergic innervations from the PAM neurons (Mao and Davis, 2009). The Drosophila MB has been studied extensively as a central brain hub for olfactory associative memory and behavior (Heisenberg, 2003; Fiala, 2007). PAM-MB circuits are required for aversive as well as rewarding reinforcement of olfactory information (Aso et al., 2012; Burke et al., 2012; Liu et al., 2012). Further analysis of our data revealed that majority (∼55%) of flies with dFz2 knockdown in the PAM neurons fly for 1–20 s as compared to controls that can fly for 700–900 s or more. Rescue of flight deficits in flies with dFz2 knockdown in PAM neurons either by over-expression of a sodium channel (NaChBac) or a transgene encoding TH (UAS-DTH1) supports a requirement for both neural activity and dopamine release from the PAM neurons for maintenance of longer flight bouts (Figure 7). Reduced flight times are very likely due to lack of dopaminergic reinforcement during flight arising from reduced strength of PAM-MB signaling.

The role of higher brain centres in Drosophila flight has been investigated primarily in the context of visual cues, and these studies identified the central complex as a key area for visual associative learning (Ofstad et al., 2011). The flight circuit identified here appears similar to the one identified recently for the startle induced climbing response which requires PAM dopaminergic inputs to the β′ lobe (Riemensperger et al., 2013). Taken together, our findings support an emerging role for the Drosophila MB in coordinated motor behavior, previously considered unlikely (Wolf et al., 1998). Dopaminergic inputs from the PAM to the MBs might help integrate olfactory sensory information with motor behavior essential in a natural environment. Further investigations should allow a better understanding of how MB centres for re-inforcement of olfactory memory interact with the flight motor system.

Drosophila was reared on corn flour/agar media supplemented with yeast, grown at 25°C, unless otherwise mentioned in the experimental design. The pan-neuronal GAL4 driver (Elav^C155GAL4), aminergic GAL4 (DdcGAL4) (Li et al., 2000) and mushroom body drivers c305aGAL4 and mb247GAL4 were obtained from Bloomington Stock Center, Bloomington, IN. mb186bGAL4 was obtained from Anja Beatrice Freidrich (MPG, Germany). The dopaminergic GAL4 (THGAL4), serotonergic GAL4 (TRHGAL4), and two other GAL4s, NP6510GAL4 and R58E02GAL4 were generously provided by Serge Birman (CNRS, ESPCI Paris Tech, France) (Riemensperger et al., 2013). The various dopaminergic subdomain GAL4 drivers used, THC’GAL4, THC1GAL4, and THF2GAL4, were obtained from Mark N Wu (Johns Hopkins University, Baltimore) (Liu et al., 2012). The peptidergic GAL4 (P386GAL4) was obtained from Paul Taghert (Washington University, St. Louis) (Taghert et al., 2001).

UAS strains of Frizzled-2 RNAi (9739R-1(II), referred to as dFz2-IR in the text and figures) and itpr RNAi (1063-R2) were obtained from National Institute of Genetics Fly Stocks Centre, Kyoto, Japan (NIG). The UASRNAi strains for dSTIM (47073), dOrai (12221), Arrow (6707 and 36286), Dishevelled (101525), Shaggy (101538), DopECR (103494), Dop1R1 (107058), Dop1R2 (105324), Dop2R (11470, 11471), and TH (108879) were obtained from Vienna Drosophila RNAi center, Vienna, Austria (VDRC). UASRNAi strains for Frizzled-2 (BL27568, BL31390 and BL31312) were also obtained from Bloomington Stock Center, Bloomington, IN. RNAi strains are referred to as IR indicating the presence of an interference RNA.

We obtained UAS-DTH1 from Serge Birman (CNRS, ESPCI Paris Tech, France), UASAcGo (GoαQ205L) from Yu Fengwei (National University of Singapore, Singapore), UASAcGi (GiαQ205L) from Jurgen Knoblich (Institute of Molecular Biotechnology, Austria), UAS-PTX.16 from Gregg Roman (University of Houston, Texas) and UASFz2 from Stephen Cohen (Institute of Molecular Cell Biology, Singapore). AcGs (GαsQ215L) BL6490; Go RNAi, BL34653; UASNaChBac, BL9468; UASDishevelled, BL9453 (Dsh); BL9522 (Dsh^G64V), UASArmadillo^active, BL4782 (Arm^act), and UAS-Shi^ts (BL44222) were obtained from the Bloomington Stock Center, Bloomington, IN. UASdOrai⁺ (Venkiteswaran and Hasan, 2009), UASdSTIM⁺ (Agrawal et al., 2010), and UASAcGq3 (Ratnaparkhi et al., 2002) have been published. The GAL80^ts strain with two inserts of tubP-GAL80^ts on the second chromosome was generated by Albert Chiang, NCBS, Bangalore, India.

Progeny were collected upon eclosion and aged for 3–4 days. For flight tests, flies were anaesthetized on ice for 15 min and a thin metal wire was glued between the neck and thorax region with the help of nail polish. To test air-puff-stimulated flight responses, videos were recorded for 30 s after a gentle mouth-blown air puff was delivered to the tethered fly. These videos were analyzed and percentage flight times were calculated. For short flight assays 30 s was taken as 100% flight time. For the long flight assay air-puff-stimulated flight times were monitored for 15 min. For each genotype, a minimum of 30 flies were tethered and tested along with 30 control flies. Flight times of individual flies were noted, and data from a minimum of 30 flies were taken for calculation of the mean and standard error of mean (SEM). Significance testing between the raw data of control and experimental genotypes was performed with the Mann–Whitney U-test using GraphPad Prism 6 (GraphPad Software Inc, La Jolla, CA, USA). Data are represented as bar graphs of the mean percentage flight times. Diamonds inside each bar represent the flight time of individual flies.

Electrophysiological recordings were obtained from the indirect dorsal longitudinal flight muscles (DLMs) as described previously (Banerjee et al., 2004). Briefly, an un-insulated 0.127-mm tungsten electrode, sharpened by electrolysis to attain a 0.5 μm tip diameter, was inserted in the DLMs (fiber a). A similar electrode was inserted in the abdomen for reference. Air-puff stimulated recordings were obtained for 30 s. All recordings were performed using an ISO-DAM8A amplifier (World Precision Instruments, Sarasota, FL) with filter set up of 30 Hz (low pass) to 10 kHz (high pass). Gap free mode of pClamp8 (Molecular Devices, Union City, CA) was used to digitize the data (10 kHz) on a Pentium 5 computer equipped with Digidata 1322A (Molecular Devices). The duration of rhythmic action potential was analyzed using Clampfit (Molecular Devices) and the mean and standard error (SEM) were plotted using Origin 8.0 software (MicroCal, Origin Lab, Northampton, MA, USA). Spike durations in individual flies have been represented as diamonds within the histograms.

Progeny were collected upon eclosion and aged for 3–4 days. To test for climbing, flies in batches of 10 were transferred into cylinder of diameter 2.5 cm. Numbers of flies that crossed the 8 cm mark on the cylinder within 12 s, after three gentle taps, were recorded. This procedure was repeated three times with three independent batches of flies. Means and SEM were calculated using the Origin 8.0 software (MicroCal, Origin Lab, Northampton, MA, USA).

For isolation of RNA, the central nervous system (CNS) was dissected from adult flies. For each genotype, three independent sets of RNA were isolated each from eight dissected CNS preparations. Total RNA was isolated using TRIzol Reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) according to the manufacturer's specifications. Integrity of RNA was confirmed by visualization on a 1% TAE (40 mM Tris pH 8.2, 40 mM acetate, 1 mM EDTA) agarose gel. Total RNA (500 ng) was treated with DNase in a volume of 45.5 μl with 1 μl (1U) DNase I (Amplification grade, Invitrogen Life Technologies, Carlsbad, CA, USA) with 1 mM dithiothreitol (DTT) (Invitrogen Life Technologies, Carlsbad, CA, USA), 40U of RNase Inhibitor (Promega, Madison, WI, USA) in 5X First Strand Buffer (Invitrogen Life Technologies, Carlsbad, CA, USA) for 30 min at 37°C and heat inactivated for 10 min at 70°C. The reverse transcription reaction was performed in a final volume of 50 μl by addition of 1 μl (200U) Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen Life Technologies, Carlsbad, CA, USA), 2.5 μl (500 ng) random hexaprimers (MBI Fermentas, Glen Burnie, MD, USA) and 1 μl of a 25 mM dNTP mix (GE Healthcare, Buckinghamshire, UK). Samples were incubated for 10 min at 25°C, then 60 min at 42°C and heat inactivated for 10 min at 70°C. The polymerase chain reactions (PCRs) were performed using 1 μl of cDNA as a template in a 25 μl reaction under appropriate conditions to check the integrity of cDNA prepared.

Real time quantitative PCR (qPCR) was performed on an ABI 7500 Fast machine (Applied Biosystems, Foster City, California, USA) operated with ABI 7500 software version 2 (Applied Biosystems, Foster City, California, USA) using MESA GREEN qPCR MasterMIx Plus for SYBR Assay I dTTp (Eurogentec, Belgium). Each qPCR experiment was repeated three times with independently isolated RNA samples. qPCRs were performed with rp49 primers as internal controls and primers specific to gene of interest using dilutions of 1:10. Sequences of the primers used in the 5′ to 3′ directions are given below. The sequence of the forward primer is given first in each case: dfz2 GGTTACGGAGTGCCAGTCAT; CACAGGAAGAACTTGAGGTCC, rp49 CGGATCGATATGCTAAGCTGT; GCGCTTGTTCGATCCGTA, dsh CCAAATCCCAAGGGCTACTTC; ATAATACTGTCGTGCGATGTGAG sgg GCTGCTCGAGTATACGCCC; CACTAGGCTGGGCTGTATTGA th GTTGCAGCAGCCCAAAAGAAC; GAGACCGTAATCATTTGCCTTGC.

The cycling parameters were 95°C for 5 min, 40 cycles of 95°C for 15 s, and 60°C for 1 min followed by 1 cycle of 72°C for 5 min. The fluorescent signal produced from the amplicon was acquired at the end of each polymerization step at 60°C. A melt curve was performed after the assay to check for specificity of the reaction. The fold change of gene expression in the genotype relative to wild-type was determined by the comparative ΔΔCt method (Lorentzos et al., 2003). In this method, the fold change = 2^−ΔΔCt where ΔΔCt = (C_{t(target gene)} − C_t(rp49))_mutant2 − (C_{t(target gene)} − C_t(rp49))_{Wild type}.

Immunohistochemistry was performed on Drosophila adult brains expressing cytosolic GFP (UASGFP) with the specified GAL4 strains, after fixing the dissected tissue in 4% paraformaldehyde. The following primary antibodies were used: mouse monoclonal anti-TH antibody (1:50, #22941, ImmunoStar, Hudson, WI, USA), rabbit anti-GFP antibody (1:10,000; #A6455, Molecular Probes, Eugene, OR, USA), mouse anti-Fz2 (1:20; #12A7, DSHB, University of Iowa). 12A7 was deposited to the DSHB by Nusse, R (DSHB Hybridoma Product 12A7). Fluorescent secondary antibodies were used at a dilution of 1:400 as follows: anti-rabbit Alexa Fluor 488 (#A1108) and anti-mouse Alexa Fluor 568 (#A1104, Molecular Probes, Eugene, OR, USA). After antibody staining, confocal analysis was performed on an Olympus Confocal FV1000 microscope and visualized using the FV10-ASW 1.3 viewer (Olympus Corporation, Tokyo, Japan).

Mean intensity of TH or GFP immunostaining was calculated using ImageJ Version 10.2 (U. S. National Institutes of Health, Bethesda, Maryland, USA, http://imagej.nih.gov/ij/, 1997–2014). Region of interest was drawn around each neuron and mean intensities were obtained for TH and GFP for all the neurons. Median shown by the horizontal line and spread of 25–75% of cell intensities represented as big square was calculated and plotted using Origin 8.0 software (MicroCal, Origin Lab, Northampton, MA, USA) with the data from all the neurons. Significant difference between the different groups of cell intensities was calculated using One-way analysis of variance (ANOVA) for p < 0.05.

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

We thank Dr Krishnamurthy and NCBS Central Image and Flow Facility for help with the confocal imaging. Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study.

© 2015, Agrawal and Hasan

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