The F-box and U-box E3 ubiquitin ligase decoy library is a powerful tool for the functional characterization of redundant E3 ubiquitin ligases, like MAC3A and MAC3B, novel circadian clock regulators.

RNF213 is a giant E3 ligase with a dynein-like core and a unique ubiquitination mechanism that proceeds in a RING-independent manner and is linked with the Moyamoya disease.

CRISPR/Cas9 genome-wide screens using sterol-sensitive endogenous HMG-CoA reductase (HMGCR) reporter identify the sterol-responsive RNF145 and gp78 as independently responsible for sterol-accelerated degradation of HMGCR, the rate-limiting enzyme of cholesterol biosynthesis.

Proteasome-mediated degradation of the TATA-box binding protein (TBP) during terminal differentiation is regulated by the E3 ligase Huwe1 and the deubiquitinase USP10.

The identification of a SUMO E3 ligase engaged in lncRNA transcription, subsequently leading to target-directed small RNA degradation, reveals an unexplored layer of regulatory mechanisms within small RNA-directed chromatin regulation.

Arrestin-related trafficking adaptors (ARTs) undergo a K63 linked di-ubiquitin modification to promote Rsp5 E3 ligase recruitment and enhance cargo protein ubiquitination.

Cyclin D1 protein stability is regulated by ubiquitin-proteasome mechanism mediated by multiple E3 ligases, including newly identified Keap1, DDB2, and WSB2.

Simultaneous phosphorylation of multiple substrate motifs drives switch-like target recognition and monoubiquitylation by an E3 ligase during metazoan development.

A single transmembrane E3 ligase complex can regulate protein ubiquitination at multiple organelles using interchangeable targeting subunits.

The abundance of the PIF4 transcription factor, central in light and temperature signaling, is controlled by an E3 ligase encoded by BOP proteins.