A molecular model that provides a framework for interpreting the wealth of functional information obtained on the E. coli F-ATP synthase has been generated using cryo-electron microscopy.
E. coli ribosomes incapable of base-pairing with the Shine-Dalgarno sequence are still selective for annotated start sites, indicating these sites are hard-wired for initiation by other mRNA features.
Meghna Sobti, Robert Ishmukhametov ... Alastair G Stewart
Cryo-EM studies reveal that incubation with ATP produces conformational intermediates of E. coli ATP synthase, in which the ε subunit is no longer in its autoinhibitory conformation.
Sharon A McGrath-Morrow, Jarrett Venezia ... Alan L Scott
A pre-clinical model of E. coli pneumonia reveals attenuated dynamics of MHCII-expressing mononuclear cells in neonatal lung associated with elevated susceptibility to bacterial pneumonia.
Single-cell measurements combined with a new statistical framework for discriminating between models of cell cycle regulation show that chromosome initiation controls the E. coli cell cycle via two adder mechanisms.
Cryo-electron microscopy structures, combined with biochemical experiments, show how the E. coli F element-encoded TraR protein regulates transcription initiation by altering RNA polymerase conformation and conformational heterogeneity.