Genomic editing and replacement of yeast ORFs is described in greater detail at Bio-protocol (Akhmetov et al., 2018).
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A more detailed protocol is described at Bio-Protocol (Karuna et al., 2018).
Dimethyl suberimidate-crosslinking of nucleosomes is described in more detail at Bio-protocol (Ichikawa and Kaufman, 2018).
no-eIF2B control condition.
Eugenia Cammorota Pietro Cicuta
The synergy site encompasses the DRVPPSRN sequence in mouse FN and site directed mutagenesis identified the two arginine residues to be essential for all synergy site-induced functions (Aota et al., 1994; Friedland et al., 2009; Chada et al., 2006; Nagae et al., 2012).
The fluorescent dye method is described more in detail at Bio-protocol (Rigosa et al., 2018).
Detailed protocol for registration can be found at Bio-protocol (Zhuang et al., 2018).
Strain generation and fitness assays are described in greater detail at Bio-protocol (Calarco and Norris, 2018).
Procedures of dissection and dye loading for VSD imaging are described in more detail at Bio-protocol (Tomina and Wagenaar, 2018).