Roya E Huang, Giridhar M Anand ... Sharad Ramanathan
A method for arrayed CRISPRi screening in organoids enables the investigation of morphogenesis in human stem cell-derived tissues, revealing the roles of ZIC2, SOX11, and ZNF521 in neural tube closure.
Systematic characterization of terminating ribosomes uncovers sequence features influencing ribosome dynamics at stop codons with direct implications in 3' UTR translation.