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Cutting Edge: Using mobile sequencers in an academic classroom

  1. Sophie Zaaijer
  2. Columbia University Ubiquitous Genomics 2015 class
  3. Yaniv Erlich Is a corresponding author
  1. Columbia University, United States
  2. New York Genome Center, United States
  3. Center for Computational Biology and Bioinformatics, Columbia University, United States
Feature Article
Cite as: eLife 2016;5:e14258 doi: 10.7554/eLife.14258
2 figures, 1 table and 7 additional files


The Ubiquitous Genomics class.

(A) Illumina MiSeq benchtop sequencer (left) and the MinION sequencer made by Oxford Nanopore Technologies (right; red rectangle). (B) The class during a hackathon.
A detailed workflow for running a hackathon using a MinION sequencer.


Table 1

MinION consumables:Total cost estimate (in US Dollars) is for one MinION run per team. For the complete list of equipment and consumables required for organizing a hackathon, please see the following link:
CompanyProductCat noPrice per unit (USD)Unit quantityAmount needed for ONT protocolCost
NEBUltra™ End Repair/dA-Tailing ModuleE7442S$225.0072 ul3 μl$9.40
AgencourtAMPure XPA63880$315.005 ml60 μl$3.78
Thermo FisherDynabeads MyOne Streptavidin C165001$475.002 mL50 μl$12
NEBBlunt/TA Ligase Master MixM0367S$95250ul50 μl$19
Tubes/ pipette-tips/H2O/
ethanol etc
Reagent kit
Projected cost per team per run:$981.68

Additional files

Supplementary file 1

Class website information.
Supplementary file 2

Protocol for setting up a hackathon using MinION.
Supplementary file 3

Student handout for Hackathon 1: Snack to sequence.
Supplementary file 4

Student handout for Hackathon 2: CSI Columbia.
Supplementary file 5

Student handout for the final project.
Supplementary file 6

Teaching slides for Hackathon 1: Snack to sequence.
Supplementary file 7

Teaching slides for Hackathon 2: CSI Columbia.

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