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Cutting Edge: Using mobile sequencers in an academic classroom

  1. Sophie Zaaijer
  2. Columbia University Ubiquitous Genomics 2015 class
  3. Yaniv Erlich  Is a corresponding author
  1. Columbia University, United States
  2. New York Genome Center, United States
  3. Center for Computational Biology and Bioinformatics, Columbia University, United States
Feature Article
Cite this article as: eLife 2016;5:e14258 doi: 10.7554/eLife.14258
2 figures, 1 table and 7 additional files


The Ubiquitous Genomics class.

(A) Illumina MiSeq benchtop sequencer (left) and the MinION sequencer made by Oxford Nanopore Technologies (right; red rectangle). (B) The class during a hackathon.
A detailed workflow for running a hackathon using a MinION sequencer.


Table 1

MinION consumables:Total cost estimate (in US Dollars) is for one MinION run per team. For the complete list of equipment and consumables required for organizing a hackathon, please see the following link:
CompanyProductCat noPrice per unit (USD)Unit quantityAmount needed for ONT protocolCost
NEBUltra™ End Repair/dA-Tailing ModuleE7442S$225.0072 ul3 μl$9.40
AgencourtAMPure XPA63880$315.005 ml60 μl$3.78
Thermo FisherDynabeads MyOne Streptavidin C165001$475.002 mL50 μl$12
NEBBlunt/TA Ligase Master MixM0367S$95250ul50 μl$19
Tubes/ pipette-tips/H2O/
ethanol etc
Reagent kit
Projected cost per team per run:$981.68

Additional files

Supplementary file 1

Class website information.
Supplementary file 2

Protocol for setting up a hackathon using MinION.
Supplementary file 3

Student handout for Hackathon 1: Snack to sequence.
Supplementary file 4

Student handout for Hackathon 2: CSI Columbia.
Supplementary file 5

Student handout for the final project.
Supplementary file 6

Teaching slides for Hackathon 1: Snack to sequence.
Supplementary file 7

Teaching slides for Hackathon 2: CSI Columbia.

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