Browse annotations

Comment on Version 3

The following citation to Bio-protocol was added to the start of the Materials and Methods section titled "Sucrose gradient analysis":

Polysome analyses by sucrose gradient fractionation are described in more detail at Bio-protocol (Aboulhouda et al., 2017).

The following reference has also been added to the Reference section:

Aboulhouda, S, Di Santo, R, Therizols, G, Weinberg, D. (2017). Accurate, Streamlined Analysis of mRNA Translation by Sucrose Gradient Fractionation. Bio-protocol 7(19): e2573. DOI: https://doi.org/10.21769/BioProtoc.2573

Comment on Version 3

The following citation to Bio-protocol was added to the start of the Materials and Methods section titled "Pathogen inoculation":

Xoo inoculation described in more detail at Bio-protocol (Ke et al., 2017).

The following reference has also been added to the Reference section:

Ke, Y, Hui, S and Yuan, M. (2017). Xanthomonas oryzae pv. oryzae Inoculation and Growth Rate on Rice by Leaf Clipping Method. Bio-protocol 7(19): e2568. DOI: https://doi.org/10.21769/BioProtoc.2568.

Comment on Version 2

The following citation to Bio-protocol was added to the end of the Materials and Methods section titled "Quick-freezing and freeze replica preparation":

The method of freeze-fracture/etching is described in detail at Bio-protocol (Tsuji and Fujimoto, 2017).

The following reference has also been added to the Reference section:

Tsuji, T, and Fujimoto, T. (2017). Freeze-fracture-etching Electron Microscopy for Facile Analysis of Yeast Ultrastructure. Bio-protocol 7(18): e2556. DOI: https://doi.org/10.21769/BioProtoc.2556.

Comment on Bio-protocol methods

The authors have published a detailed protocol at Bio-protocol by which the "purified human Polθ is utilized to modify the 3’ terminal ends of RNA and DNA":

https://doi.org/10.21769/BioProtoc.2330

Comment on Version 2

The following Bio-protocol citation has been added to the beginning of the "Producing GST-fusion proteins from E. coli" section in the Materials and methods:

Producing GST-Cbx7 fusion proteins is described in more detail at Bio-protocol (Huynh and Ren, 2017).

The following Bio-protocol citation has been added to the beginning of the "Labelling HaloTag fusion proteins with HaloTag ligand in living cells" section in the Materials and methods:

Labelling HaloTag Fusion Proteins is described in more detail at Bio-protocol (Huynh and Ren, 2017).

The following citations have been added to the Reference list:

Huynh, TN and Ren, X. (2017). Producing GST-Cbx7 Fusion Proteins from Escherichia coli. Bio-protocol 7(12): e2333. DOI: https://doi.org/10.21769/BioProtoc.2333.

Huynh, TN and Ren, X. (2017). Labelling HaloTag Fusion Proteins with HaloTag Ligand in Living Cells. Bio-protocol 7(17): e2526. DOI: https://doi.org/10.21769/BioProtoc.2526.

Two subsequent publications in eLife reach different conclusions around the role of MELK:

CRISPR/Cas9 mutagenesis invalidates a putative cancer dependency targeted in on-going clinical trials, by Lin et al (March 2017), https://doi.org/10.7554/eLife.24179

MELK is not necessary for the proliferation of basal-like breast cancer cells, by Huang et al (September 2017), https://doi.org/10.7554/eLife.26693

Comment on Version 3

The following citation to Bio-protocol was added to the beginning of the Materials and Methods section titled "Expression and purification":

"A detailed protocol for expression and purification of pdP2X7 is available at Bio-protocol (Karasawa and Kawate, 2017). In brief, ..."

The following citation has also been added to the Reference list:

Karasawa, A and Kawate, T. (2017). Expression and Purification of a Mammalian P2X7 Receptor from Sf9 Insect Cells. Bio-protocol 7(17): e2544. DOI: https://doi.org/10.21769/BioProtoc.2544

Comment on Version 3

The following citation to Bio-protocol was added to the beginning of the Materials and Methods section titled "siRNA screen":

The multiplex image-based autophagy RNAi screen is described in more detail at Bio-protocol (Jung and Behrends, 2017).

The following citation has also been added to the Reference list:

Jung, J and Behrends, C. (2017). Protocol for Establishing a Multiplex Image-based Autophagy RNAi Screen in Cell Cultures. Bio-protocol 7(17): e2540. DOI: https://doi.org/10.21769/BioProtoc.2540

Comment on Version 3

The following citation to Bio-protocol was added to the end of the Materials and Methods section titled "Cloning and mutagenesis":

Cloning procedures, as well as protein expression, crystallization and structure solution methods, are described in more detail at Bio-protocol (Imelio et al., 2017).

The following citation has also been added to the Reference list:

Imelio, JA, Larrieux, N, Mechaly, AE, Trajtenberg, F, Buschiazzo, A. (2017). Snapshots of the Signaling Complex DesK:DesR in Different Functional States Using Rational Mutagenesis and X-ray Crystallography. Bio-protocol 7(16): e2510. DOI: https://doi.org/10.21769/BioProtoc.2510.

Comment on Version 2

A citation to Bio-protocol (Ewald et al., 2017) was added to the end of the "Oxidative stress assays" section in the Materials and methods:

Both oxidative stress tolerance assays are described in more detail at Bio-protocol (Ewald et al., 2017).

The following citation has been added to the Reference list:

Ewald, CY, Hourihan, JM and and Blackwell, TK. (2017). Oxidative Stress Assays (arsenite and tBHP) in Caenorhabditis elegans. Bio-protocol 7(13): e2365. DOI: https://doi.org/10.21769/BioProtoc.2365.