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Comment on Version 2

The following citation to Bio-protocol was added to the end of the Materials and Methods section titled "Spore recovery and sterilisation":

A more detailed description of this method can be found on Bio-Protocol (Bosch and Djamei, 2017).

A second citation to Bio-protocol was added to the end of the Materials and Methods section titled "Differential expression and overrepresentation analyses in the transcriptomic dataset”:

A more detailed description of the methodology can be found on Bio-Protocol (Czedik-Eysenberg et. al., 2017).

The following two citations have also been added to the Reference list:

Bosch, J and Djamei, A. (2017). Isolation of Ustilago bromivora Strains from Infected Spikelets through Spore Recovery and Germination. Bio-protocol 7(14): e2392. DOI: https://doi.org/10.21769/BioProtoc.2392

Czedik-Eysenberg, A, Rabe, F, Ekker, H, Czepe, C and Djamei A. (2017). Overrepresentation Analyses of Differentially Expressed Genes in the Smut Fungus Ustilago bromivora during Saprophytic and in planta Growth. Bio-protocol 7(15): e2426. DOI: https://doi.org/10.21769/BioProtoc.2426

Comment on Version 3

The following citation to Bio-protocol (Liu et al., 2017) has been added in the Materials and methods:

The locomotor assay in Drosophila are described in more detail at Bio-protocol (Liu et al., 2017).

The following citation has also been added to the Reference list:

Liu, Q, Tian, J, Yang, X, Li, Y and Guo A. (2017). Locomotor Assay in Drosophila melanogaster. Bio-protocol 7(10): e2283. DOI: https://doi.org/10.21769/BioProtoc.2283

Co-submitted with Sandeep Dembla et al., "Anti-nociceptive action of peripheral mu-opioid receptors by G-beta-gamma protein-mediated inhibition of TRPM3 channels" –available at https://elifesciences.org/articles/26280 – and Tibor Rohacs et al., "Inhibition of Transient Receptor Potential Melastatin 3 ion channels by G-protein βγ subunits" –available at https://elifesciences.org/articles/26147.

Co-submitted with Talisia Quallo et al., "G protein βγ subunits inhibit TRPM3 ion channels in sensory neurons" – available at https://elifesciences.org/articles/26138 – and Sandeep Dembla et al., "Anti-nociceptive action of peripheral mu-opioid receptors by G-beta-gamma protein-mediated inhibition of TRPM3 channels" – available at https://elifesciences.org/articles/26280.

Co-submitted with Tibor Rohacs et al., "Inhibition of Transient Receptor Potential Melastatin 3 ion channels by G-protein βγ subunits" – available at https://elifesciences.org/articles/26147 – and Talisia Quallo et al., "G protein βγ subunits inhibit TRPM3 ion channels in sensory neurons" – available at https://elifesciences.org/articles/26138).

Comment on Version 3

A citation to Bio-protocol (Petersen and Berg, 2017) was added to the following sentence in the Materials and methods:

The experimental procedures are described in more detail at Bio-protocol (Petersen and Berg, 2017).

The following citation has been added to the Reference list:

Petersen, P and Berg, R. (2017). Lognormal firing rate distribution reveals prominent fluctuation–driven regime in spinal motor networks. Bio-protocol 7(13): e2381. DOI: https://doi.org/10.21769/BioProtoc.2381

Comment on Version 2

The following citation to Bio-protocol has been added in the Materials and methods:

The behavioral task, protocol, and analysis are described in more detail at Bio-protocol (Lesburguères et al, 2017).

The following citation has also been added to the Reference list:

Lesburguères, E, Tsokas, P, Sacktor, TC and Fenton AA. (2017) The Object Context-place-location Paradigm for Testing Spatial Memory in Mice. Bio-protocol 7(8): e2231. DOI: https://doi.org/10.21769/BioProtoc.2231

Comment on Version 2

A citation to Bio-protocol (Ho and Frommer, 2016) was added to the Fluorimetry section in the Materials and methods:

Fluorimetric analyses are described in more detail at Bio-protocol (Ho and Frommer, 2016).

The following citation has been added to the Reference list:

Ho, C-H and Frommer, WB. (2016). Design and Functional Analysis of Fluorescent Nitrate and Peptide Transporter Activity Sensors in Yeast Cultures. Bio-protocol 6(3): e1728. DOI: https://doi.org/10.21769/BioProtoc.1728

Related manuscript: Delgado et al., 2017. "Pharmacological evidence for a metabotropic glutamate receptor heterodimer in neuronal cells". eLife. Available at: https://elifesciences.org/articles/25233

Comment on Version 3

The following citation to Bio-protocol was added to the Materials and Methods:

A detailed protocol for the isolation of cytosol, microsome, free polysomes (FP) and membrane-bound polysomes (MBP) may be found at Bio-protocol (Zhao and Li, 2017).

The following citation was added to the Reference list:

Zhao, Y and Li S. (2017). Isolation of Cytosol, Microsome, Free Polysomes (FPs) and Membrane-bound Polysomes (MBPs) from Arabidopsis Seedlings. Bio-protocol 7(15): e2436. DOI: https://doi.org/10.21769/BioProtoc.2436