As the Arctic continues to warm, woody shrubs are expected to expand northward. This process, known as “shrubification”, has important implications for regional biodiversity, food web structure, and high-latitude temperature amplification. While the future rate of shrubification remains poorly constrained, past records of plant immigration to newly deglaciated landscapes in the Arctic may serve as useful analogues. We provide one new postglacial Holocene sedimentary ancient DNA (sedaDNA) record of vascular plants from Iceland and place a second Iceland postglacial sedaDNA record on an improved geochronology; both show Salicaceae present shortly after deglaciation whereas Betulaceae first appears more than 1000 years later. We find a similar pattern of delayed Betulaceae colonization in eight previously published postglacial sedaDNA records from across the glaciated circum North Atlantic. In nearly all cases, we find that Salicaceae colonizes earlier than Betulaceae and that Betulaceae colonization is increasingly delayed for locations farther from glacial-age woody plant refugia. These trends in Salicaceae and Betulaceae colonization are consistent with the plant families’ environmental tolerances, species diversity, reproductive strategies, seed sizes, and soil preferences. As these reconstructions capture the efficiency of postglacial vascular plant migration during a past period of high-latitude warming, a similarly slow response of some woody shrubs to current warming in glaciated regions, and possibly non-glaciated tundra, may delay Arctic shrubification and future changes in the structure of tundra ecosystems and temperature amplification.
This valuable work on the paleovegetation history of Iceland has implications for the field of paleoecology, and the deglaciation history of Iceland and additional localities in Northern America and Europe via woody shrub colonization. The study uses a sedimentary ancient DNA metabarcoding approach to study this historic process. The strength of evidence is solid, with the methods (analysis of sedimentary DNA) and data analyses broadly supporting the claims.
As the planet warms, the Arctic is warming at 2 to 4 times the global rate (Rantanen et al., 2022). This warming is impacting Arctic ecosystems through a process known as ‘shrubification’, or the northward expansion and increased height and density of deciduous woody vegetation (Tape et al., 2006; Myers-Smith et al., 2011; Elmendorf et al., 2012; Sweet et al., 2015). Arctic shrubification alters the structure of tundra ecosystems, its regional biodiversity, food web structure, and nutrient availability (Elmendorf et al., 2012; Fauchald et al., 2017; Collins et al., 2018). Arctic shrubification also has implications for the climate system by reducing surface albedo (Sturm et al., 2005) and increasing atmospheric water vapor (Pearson et al., 2013) – both positive feedbacks that amplify high-latitude warming (Thompson et al., 2022). However, large uncertainties remain regarding the rate of future shrubification, such that the magnitude of these positive feedbacks is poorly constrained in predictive earth system models. One way to reduce future climate uncertainties is through the analysis of well-constrained paleoenvironmental records dating to warmer-than-present periods in Earth’s recent history (e.g., Tierney et al., 2020).
Traditionally, macrofossils and pollen have formed the backbone of Quaternary paleovegetation reconstructions (Birks, 2018). However, macrofossils are not consistently preserved in sedimentary records, and pollen production is restricted to seed-bearing taxa, which can be obscured by long-distant transport from sources 1000s of km away (Hyvärinen, 1970; Birks, 2003; Crump et al., 2019). For example, in Iceland, exotic tree pollen from taxa never reported as macrofossils since the Miocene and Pliocene (i.e., Pinus, Quercus, Corylus, Alnus, and Ulmus, Denk et al., 2011a, b) are found in Early Holocene sedimentary records following retreat of the Icelandic Ice Sheet (Caseldine et al., 2006; Eddudóttir et al., 2015). Hence, the first appearance of pollen need not reflect local colonization of a given taxon. Recent analytical advances in sedimentary ancient DNA (sedaDNA) allow for more reliable vegetation reconstructions (e.g., Capo et al., 2021), due in part to their application in continuous lake sediment records that capture colonization times following local deglaciation. Taxa identified by DNA in lake surface sediment closely match those in catchment vegetation cover, confirming the local origin of DNA in lake sediment and improved reliability for taxa presence compared to pollen (Sjögren et al., 2017; Alsos et al., 2018). Past records of vascular plant sedaDNA can therefore provide new insight into postglacial colonization patterns (Crump et al., 2019; Alsos et al., 2022) as well as the response of vegetation to changes in temperature during prior warm periods (Clarke et al., 2020; Crump et al., 2021; Huang et al., 2021).
In this study we present one new Icelandic record (Stóra Viðarvatn) and update the age model of a second previously published record from Iceland (Torfdalsvatn, Alsos et al., 2021). For these two lakes, we also compare existing pollen and sedaDNA datasets to highlight the ability of sedaDNA in pinpointing the timing of each taxon’s first appearance. We then compare sedaDNA records from eight other published North Atlantic lakes that track local vegetation assemblages since deglaciation, with particular attention to postglacial colonization patterns of two woody plant families, Salicaceae and Betulaceae. Collectively, the North Atlantic vascular plant DNA data sets demonstrate that Salicaceae colonized deglaciated lands quickly, whereas Betulaceae was delayed by up to several thousand years. As these colonization patterns took place during a period of high latitude warming, our datasets suggest that ongoing warming may also lead to different northward expansion rates of woody shrubs in glaciated regions, and possibly tundra regions as well. As a result, the rate of Arctic shrubification may be delayed, with important consequences for high-latitude ecosystems and temperature amplification.
Results and interpretation
Stóra Viðarvatn, Iceland
Stóra Viðarvatn (#4, Table 1, Figure 1) is a large lake (surface area: 2.4 km2, depth: 48 m) in northeast Iceland, with no pre-existing records of Holocene vegetation change. In winter 2020, we recovered sediment core 20SVID-02 from 17.4 m water depth near the center of the lake. The core contains 893 cm of sediment, collected in 1.5 m increments. The basal 20 cm are laminated, clay-rich sediment, transitioning to organic gyttja by 873 cm depth, which characterizes the remainder of the record, interbedded with tephra (volcanic ash) deposits (Figure S1). The chronology of the Early to Middle Holocene portion of the record is based on 5 tephra layers (see Materials and Methods), of which the lowest, at 886.5 cm depth, is identified as the Askja S tephra (10830 ± 57 BP, Bronk Ramsey et al., 2015), and the highest, at 334 cm depth, as Hekla 4 (4200 ± 42 BP, Dugmore et al., 1995). Because the sedimentation rate between the six tephra layers is essentially linear, we extrapolate that rate from the Askja S tephra layer 7 cm to the base of the sediment core at 893.5 cm depth, which provides a minimum deglaciation age of ∼10950 BP (Figure S2A).
Samples for sedaDNA analysis were taken from 75 equi-spaced depths throughout core 20SVID-02. Of these, 73 yielded amplifiable vascular plant DNA using the trnL P6 loop primer set. The two samples that failed (810 and 856.5 cm depth) were sampled within thick tephra layers with low organic content. Following data filtering (see Materials and Methods), the trnL dataset yields 15,738,794 total assigned reads, with an average of 209,851 reads per sample. qPCR cycle threshold (CT) values, which reflect PCR efficiency and the quantity of suitable target sequences for amplification, average 32.1 across samples. While there is inter-sample variation in DNA reads and CT values, linear regressions show nearly stable temporal trends (Figure S2), which indicates that the availability of DNA to PCR amplification is consistent throughout the record. Metabarcoding technical quality (MTQ) and analytical quality (MAQ) scores are below suggested thresholds for low quality (0.75 and 0.1, respectively, Rijal et al., 2021) only in some samples during the Early Holocene (Figure S2). However, given that the scores correlate with species richness due to the latter values all falling below 30 (Figure S2), the low MTQ and MAQ scores are likely an artifact of the requirement that the 10 best represented barcode sequences are required for calculation (Rijal et al., 2021), and not an indication of low-quality DNA. As PCR amplification results in sequence read abundances that may not reflect original relative abundances in a sample (Nichols et al., 2018), we focus our discussion on taxa presence/absence, with a greater number of replicates representing a higher likelihood of taxa presence. We identified 53 taxa throughout Stóra Viðarvatn’s lake sediment record. Of the key woody shrub taxa, Salicaceae is present at the base of the core (892.5 cm depth, 10850 BP) and is present nearly continuously through the core. Betulaceae first appears ∼2300 years later (701.5 cm depth, 8560 BP), and has a sporadic presence until the Middle Holocene at ∼5500 BP (Figure 2A).
Torfdalsvatn (#3, Table 1, Figure 1) is a small lake (surface area: 0.41 km2, depth: 5.8 m) in north Iceland from which sediment cores have been analyzed since the early 1990s to create paleoecological records from pollen and macrofossils (Björck et al., 1992; Rundgren, 1995, 1998), and sedaDNA (Alsos et al., 2021). The paleovegetation records derived from pollen/macrofossils and sedaDNA were constructed from separate sediment cores that have independent age models derived from radiocarbon and tephra layers of known age. To temporally compare the two sediment records, radiocarbon ages need to be calibrated using the same calibration curve, correlative tephra layer ages must agree, and age model construction requires a common method (i.e., linear interpolation vs. Bayesian). For the pollen/macrofossil records (Rundgren 1995, 1998), this required calibrating the radiocarbon ages using IntCal20 (Reimer et al., 2020) and application of a Bayesian age modeling approach that informs sediment rate changes with prior information (Blaauw and Christen, 2011) (see Geirsdóttir et al., 2020). For the sedaDNA record (Alsos et al., 2021), we revised the tephra ages previously used to establish a more reliable Bayesian age model (see Methods and Materials). In doing so, the sedaDNA and pollen records feature similar linear sedimentation rates and are now synchronized by the G10ka Series tephra layers (Figure S2C). The revised age model for the Early Holocene portion of the pollen/macrofossil record (Figure S2B, Geirsdóttir et al., 2020) sets the timing of deglaciation, inferred from the onset of organic sedimentation at 801 cm depth, to 11800 BP.
Differences on the order of centuries between the pollen/macrofossil and sedaDNA records may be artifacts of chronological uncertainty. This is because although the revised age models for the pollen/macrofossil and sedaDNA records feature common tephra layers, variable levels of proxy data resolution between the two records increases uncertainty when comparing the timing of inferred changes. Pollen of both Salicaceae (Salix spp.) and Betulaceae (Betula spp.) first appear by ∼11800 BP (Figure 3). The oldest B. nana macrofossil dates to ∼9300 BP, similar to macrofossils of S. herbacea (∼9400 BP) and S. phylicifolia (∼9300 BP) (Figure 3). The trnL primed sedaDNA data indicate that Salicaceae did not colonize Torfdalsvatn’s catchment until ∼10300 BP, whereas Betulaceae arrived later at ∼9500 BP (Figure 3).
Resolving Icelandic records of woody taxa colonization
Based on the original age model, Torfdalsvatn pollen records were interpreted to capture ecological changes associated with the abrupt climate oscillations between the Bølling-Allerød (14700 to 12900 BP) and Younger Dryas (12900 to 11700 BP) periods (Björck et al., 1992; Rundgren, 1995). However, recent calibration of the radiocarbon ages in the Torfdalsvatn cores from Björck et al. (1992) and Rundgren (1995) suggest that the organic-rich portion of the record began less than 11800 years ago (Figure S2B, Geirsdóttir et al., 2020). While there is sediment at deeper levels, it is described as silty clay (Björck et al., 1992) with sand and gravel (Rundgren, 1995). These characteristics suggest that the sediment likely originates from a rapidly deglaciating environment, perhaps with lingering ice sheet meltwater in the catchment and a rapid sediment accumulation rate that is difficult to constrain without secure age control. Therefore, we suggest that the maximum age for the final retreat of the Icelandic Ice Sheet from Torfdalsvatn’s catchment, when organic-rich sedimentation began, is ∼11800 BP, several thousand years younger than previously proposed (Björck et al., 1992; Rundgren, 1995). We use the updated age models to reconstruct the history of plant colonization in Iceland.
Differences in taxa presence/absence between the sedaDNA and pollen records from Torfdalsvatn likely reflect different mechanisms of transport for DNA and pollen. SedaDNA for both Salicaceae and Betulaceae from Torfdalsvatn shows a colonization delay relative to deglaciation, whereas pollen from both taxa is present, albeit in low abundance, in the oldest sediment samples (Figure 3). This is most likely explained by the local catchment origin of sedaDNA (Sjögren et al., 2017) versus pollen, which can be transported by wind from 1000s of km away (Caseldine et al., 2006; Eddudóttir et al., 2015). Therefore, the occurrence of low pollen counts prior to the corresponding identification with sedaDNA (Alsos et al., 2021) likely reflects long-distance transport from sources outside of Iceland rather than local establishment in the catchment – an inference supported by a lack of Salix and Betula macrofossils during this interval (Figure 3, Rundgren, 1998). While poor DNA preservation could explain the delay in sedaDNA, other vascular plants are identified before Salicaceae and Betulaceae DNA appear, suggesting adequate DNA preservation for PCR amplification (Alsos et al., 2021). Moreover, the corresponding sediment is rich in clay (Rundgren, 1995), which preferentially binds with and stabilizes DNA (Kanbar et al., 2020), making poor preservation unlikely. Therefore, we conclude that the colonization of Salicaceae and Betulaceae were delayed by 1500 and 2300 years, respectively, after local deglaciation of Torfdalsvatn’s catchment.
Relative to Torfdalsvatn, the catchment of Stóra Viðarvatn deglaciated ∼1000 years later at ∼10850 BP (Figure 2A). Based on our new sedaDNA record, Salicaceae colonized the catchment rapidly, as it was identified in the lowermost sample at ∼10850 BP, whereas Betulaceae does not appear in the record until 8560 BP, more than 2000 years later. Considering CT values are relatively stable throughout the entire Holocene record (Figure S3), the delayed appearance of Betulaceae does not likely result from preservation bias. While Stóra Viðarvatn does not have any independent paleovegetation records, a pollen record from a nearby peat section Ytra-Áland in Thistilfjörður (Karlsdóttir et al., 2014), ∼12 km to the east, provides a useful cross-examination of woody shrub colonization patterns. In the Ytra-Áland record, Salix pollen is found in high counts well above the mean in the lowermost sample dated to 10400 BP, suggesting bona fide early colonization (Figure 4). For Betula, there is limited to no pollen present until ∼8000 BP, after which there is sporadic presence as we observe with sedaDNA (Figure 4). The Betula pollen-limited period at Ytra-Áland is followed by the first peak of Betula presence in the total land pollen during ∼7700 to 6800 BP. According to size differentiation between the two Betula species in Iceland (Karlsdóttir et al., 2007), the pollen recovered in this peak period is mostly from the dwarf birch B. nana, but with a fair amount of pollen from the shrub-like B. pubescens, suggesting arctic-alpine heath with stands of downy birch in the region (Karlsdóttir et al., 2014). Despite the coarser resolution of Ytra-Áland’s age model, which only has two tephra layer dates bounding the timing of first appearance of these taxa (10400 BP and 7050 BP, Karlsdóttir et al., 2014), there is reasonable agreement between lake and peat records for the timing of arrival and subsequent Early Holocene establishment of Salicaceae and Betulaceae in northeast Iceland.
Based on the sedaDNA records from Torfdalsvatn and Stóra Viðarvatn, we find that as the Icelandic Ice Sheet receded and exposed new land Salicaceae colonized the landscape. This occurred 0 to 1700 years after inferred deglaciation, whereas Betulaceae colonization was further delayed by a few thousand years (∼2300 years in both cases). As Torfdalsvatn is the oldest dated terrestrial site of deglaciation in Iceland, this implies that ∼1700 years was required for Salicaceae to establish a viable population on the island. By the time Stóra Viðarvatn deglaciated, it is likely that other portions of north Iceland had also deglaciated allowing Salicaceae to more efficiently colonize new regions of Iceland. The similar timing of Betulaceae colonization relative to deglaciation in the two catchments (∼2300 years) but different times of deglaciation (11800 and 10850 BP) suggests that the evolution of catchment conditions for Torfdalsvatn and Stóra Viðarvatn may be additional factors controlling the local colonization of this taxon on Iceland. The earlier presence of low concentrations of Salicaceae and Betulaceae pollen in Torfdalsvatn are most likely derived from long distance wind dispersal from outside Iceland.
Postglacial sedaDNA records from the circum North Atlantic
Eight additional records of vascular plant sedaDNA from lake sediments located around the North Atlantic that were glaciated during the Last Glacial Maximum are available to compare with the Icelandic data (see Material and Methods for details). These include records from Baffin Island (n=1, Crump et al., 2019), northern Greenland (n=1, Epp et al., 2015), Svalbard (n=2, Alsos et al., 2016; Volstad et al., 2020), northern Norway (n=3, Rijal et al., 2021; Alsos et al., 2022), and southern Norway (n=1, ter Schure et al., 2021) (Figure 1). All eight records have age control derived from calibrated radiocarbon ages. Except for Baffin Island, we infer minimum ages of deglaciation based on the age of the oldest sediment.
Baffin Island: Lake Qaupat (#1, Figure 1) is a small lake (surface area: 0.08 km2, depth: 9.2 m) situated at 35 m asl on southern Baffin Island. Cosmogenic 10Be exposure dating of Lake Qaupat’s impounding moraine constrains the timing of deglaciation to 9100 ± 700 BP (Crump et al., 2019). Lake Qaupat and most of its catchment were below sea level until 7700 ± 300 BP, when postglacial isostatic recovery raised the basin above the ocean and its catchment was available for vascular plant colonization. Salicaceae is present in the record at shortly after isolation from the ocean at 7400 BP, but no vascular plants are documented by sedaDNA in the older marine sediment. The first appearance of Betulaceae sedaDNA occurs at 5900 BP, more than 1000 years after Salicaceae and 2000 years after the lake and its catchment were above sea level (Crump et al., 2019).
Greenland: Bliss Lake (#2, Figure 1) is a small lake (depth: 9.8 m) situated at 17 m asl on the northern coastline of Greenland (Peary Land). Bliss Lake initially deglaciated 11000 BP (Olsen et al., 2012) and the first appearance of Salicaceae sedaDNA is found in low read counts in sediment dated to 10800 BP but only in high read counts beginning at 7400 BP (Epp et al., 2015). As the lake was inundated by marine water between 10480 and 7220 BP, (Olsen et al., 2012), 7400 BP is interpreted to be the most reliable date for Salicaceae colonization in the catchment as sea level regressed and isolated the lake from the ocean (Epp et al., 2015).
Svalbard: Jodavannet (#5, Figure 1) is a small lake (depth: 6.4 m) situated at 140 m asl on the east coast of Wijdefjorden on northern Spitsbergen. Regional cosmogenic exposure dating suggests the area began to deglaciate between 14600 and 13800 BP (Hormes et al., 2013), although the base of Jodavannet’s sediment record suggests a minimum timing of lake deglaciation by 11900 BP (Volstad et al., 2020). Salicaceae sedaDNA is first found at 9800 BP in Jodavannet, whereas Betulaceae was not detected with sedaDNA metabarcoding (Volstad et al., 2020). Lake Skartjørna (#6, Figure 1) is a small lake (surface area: 0.10 km2, depth: 7.5 m) situated at 65 m asl on the west coast of Spitsbergen. As sea level fell due to glacial isostatic adjustment, Lake Skartjørna likely became isolated from the sea at ∼13000 BP (Landvik et al., 1987), although the base of the recovered sediment record, which provides a minimum deglaciation age, is dated to 8600 BP (Alsos et al., 2016). Salicaceae sedaDNA is identified in Lake Skartjørna’s basal sample at 8600 BP and Betulaceae is first identified at 7000 BP (Alsos et al., 2016).
Norway: Langfjordvannet (#7, Figure 1) is a small lake (surface area: 0.55 km2, depth: 34.8 m) situated at 66 m asl on the coast of northern Norway (Rijal et al., 2021). Langfjordvannet deglaciated by 16150 BP, Salicaceae is first identified in sedaDNA by 15500 BP, and Betulaceae by 10000 BP (Alsos et al., 2022). Eaštorjávri South (#8, Figure 1) is a small lake (surface area: 0.06 km2, depth: 5.4 m) situated at 260 m asl near the coast of northern Norway (Rijal et al., 2021). Eaštorjávri South deglaciated by 11060 BP, with Salicaceae sedaDNA identified in the basal sample at 11060 BP and consistently present above that level. Betulaceae is identified, albeit with low read counts, in the basal sample as well, but not identified in the overlying sample. Since Betulaceae sedaDNA is not present with substantial read counts until 9950 BP (Alsos et al., 2022), we conservatively set this as Betulaceae’s first appearance in Eaštorjávri South. Nordvivatnet (#9, Figure 1) is a small lake (surface area: 0.05 km2, depth: 13.3 m) situated at 82 m asl on the coastline of northern Norway (Rijal et al., 2021). Nordvivatnet deglaciated by 12880 BP, with Salicaceae sedaDNA identified in the basal sample. Betulaceae sedaDNA is identified in the basal sample as well but below our cut-off for taxa presence (see Materials and Methods). The first sample with high read counts indicative of Betulaceae presence is at 12080 BP (Alsos et al., 2022), which we conservatively set as the timing of the taxa’s first appearance in Nordvivatnet. Finally, Lake Ljøgottjern (#10, Figure 1) is a small lake (surface area: 0.02 km2, depth: 18 m) situated at 185 m asl in southeastern Norway. Lake Ljøgottjern deglaciated by 9300 BP and both Salicaceae and Betulaceae sedaDNA were first detected by 7900 BP (ter Schure et al., 2021).
Betulaceae colonization is delayed relative to Salicaceae in the circum North Atlantic
The timing of postglacial Salicaceae and Betulaceae colonization varies across the North Atlantic regions, with first-appearance dates ranging from 15500 BP in Norway to 5900 BP on Baffin Island (Figure 5). Salicaceae appears immediately after inferred deglaciation at 4 out of 10 sites, while colonization dates for the other 6 locations range from 200 to 2100 years after deglaciation and exhibit no clear spatio-temporal pattern (Figure 6). For Betulaceae, colonization times range from 800 to 6150 years after deglaciation, with sites closest to source refugia south of the ice sheets (Figure 1) having generally shorter colonization delays (Figure 6), consistent with seedling viability having a higher probability of success with shorter distance from source (Nathan, 2006). The one exception is Langfjordvannet, in northern Norway (site #7, Figure 1, Alsos et al., 2022), which deglaciated at 16150 BP (Figure 5). Considering that Betulaceae only arrives during the Holocene Epoch (last 11700 years) across all North Atlantic sites, the anomalously long delay of Betulaceae to Langfjordvannet suggests that this taxon may have required the Holocene’s relatively stable climate, as reflected by Greenland oxygen isotope records (Figure 5, Seierstad et al., 2014), to successfully establish itself. Alternatively, the colonization delay of Betulaceae to Langfjordvannet may reflect the slow development of stable soils, which are required for Betulaceae establishment, due to the lake’s steep catchment (Otterå, 2012). In contrast to Betulaceae, Salicaceae appears to have been an efficient colonizer to Langfjordvannet during the colder and less stable climate of the Late Glacial period and earliest Holocene (Figure 5, Seierstad et al., 2014). Finally, except for Lake Ljøgottjern in southern Norway (site #10, Figure 1) where Betulaceae and Salicaceae apparently colonize at about the same time (ter Schure et al., 2021), all other sites show that Betulaceae colonization is delayed by 800 to 5500 years relative to Salicaceae colonization dates (Figure 5).
Several possibilities may contribute to the delay in Betulaceae colonization compared to Salicaceae colonization. First, the northernmost Salicaceae species reach bioclimate subzone A (1-3 °C July temperature) whereas the northernmost Betulaceae species rarely go beyond bioclimate subzone D (8-9 °C July temperature) (Walker et al., 2005). The different environmental tolerances may explain why Salicaceae generally arrives earlier when regional temperatures are still climbing compared to the timing of Betulaceae colonization when warmth is more broadly established (Figure 5). In support, Alsos et al. (2022) showed that at least for Fennoscandian sedaDNA vascular plant records, temperature was one of the dominant controls on postglacial dispersal patterns. Second, species diversity is greater for the Salicaceae family compared to Betulaceae family in the North Atlantic, and conventional DNA metabarcoding techniques cannot identify sedaDNA to the species level. Iceland, for example, has four native Salicaceae taxa, i.e., three dwarf, cold-tolerant arctic species (S. herbacea, S. lanata, and S. arctica) and one shrub-like species (S. phylicifolia), and only two Betulaceae (B. nana and B. pubescens) (Kristinsson, 2008). The suite of Salicaceae taxa can collectively tolerate a wider range of environments than Betulaceae, including disturbed, wet, and nutrient limited substrate characteristic of deglacial environments (e.g., S. herbacea, Beerling, 1998). Third, the modes of reproduction for Salicaceae and Betulaceae are different. Salicaceae is clonal, meaning that plants mostly reproduce asexually by vegetative spreading, which is advantageous when colonizing new landscapes (e.g., Beerling, 1998). Betulaceae propagates instead by seed and therefore requires a larger number of founder individuals for successful establishment (Atkinsson, 1992; de Groot et al., 1997). Moreover, Betulaceae seeds are generally heavier than Salicaceae seeds (dry mass, B. nana = 0.12 to 0.30 mg, de Groot et al., 1997; S. herbacea = 0.07 to 0.14 mg, Beerling, 1998). This mass difference would provide a higher probability of long-distance wind dispersal for Salicaceae compared to Betulaceae and establishment of the pioneer plants.
Finally, modern observations broadly replicate the records of delayed woody taxa colonization in the North Atlantic. Studies tracking the migration of Salicaceae and Betulaceae in the forefields of retreating glaciers and to new islands also show a rapid and faster colonization of Salicaceae compared to Betulaceae (Whittaker, 1993; Magnússon et al., 2009; Burga et al., 2010; Synan et al., 2021). As an example, following the formation of the volcanic island Surtsey in 1964 CE, Salicaceae (S. herbaceae, S. lanata and S. phylicifolia) colonized between 1995 and 1999 CE and Betulaceae has yet to arrive (Magnússon et al., 2009). Complimentary studies tracking soil and nutrient development at proglacial sites in the North Atlantic (Vilmundardóttir et al., 2014) suggest that Salicaceae is environmentally unconstrained (Whittaker, 1993; Glausen and Tanner, 2019), whereas Betulaceae requires more developed soil for its ultimate viability (Synan et al., 2021). Collectively, these studies support the environmental constraints known for each woody taxon and reinforce the hypothesis that Salicaceae is a more efficient pioneer than Betulaceae, particularly in newly deglaciated landscapes.
Environmental dispersal mechanisms
Coupling the timing of postglacial woody shrub colonization in the North Atlantic with genetic approaches provides a valuable opportunity to identify probable environmental dispersal mechanisms (e.g., wind, sea ice, driftwood, and birds) for long-distance transport. Previously, Alsos et al. (2015) compared amplified fragment length polymorphism (AFLP) data from living plants from Iceland, Greenland, Svalbard, the Faroe Islands, and Jan Mayen to AFLP data from the same species from potential source regions. Genetic distances suggest that Betula (sp. nana and pubescens) and Salix herbacea populations in most locations descend from ancestors in a western European Last Glacial Maximum (LGM) refugia (Alsos et al., 2009; Eidesen et al., 2015), and that each taxa underwent a broad-fronted and rapid migration to newly deglaciated regions (Alsos et al., 2015). B. nana on Svalbard likely descends from a northwest Russian source (Alsos et al., 2007, 2015), and populations on Baffin Island and western Greenland originated from North America (Alsos et al., 2009; Eidesen et al., 2015). Molecular analysis of Icelandic Betula based on chloroplast haplotypes indicates that Betula may have post-glacially immigrated to Iceland from northern Scandinavia (essentially B. nana) and northwestern Europe (B. pubescens) (Thórsson et al., 2010). However, the relative roles of wind, sea ice, driftwood, and birds as transport vectors remain an open question.
Sea ice has been suggested to be a dispersal mechanism for postglacial vascular plants in the Arctic (Alsos et al., 2016). While seasonal sea ice persisted throughout the Holocene around Svalbard (Pieńkowski et al., 2021) and northern Greenland (Syring et al., 2020), general ocean circulation patterns require source populations of vascular plants in Arctic Siberia, the dominant source region for sea ice export to the North Atlantic, for sea ice to be a viable vector. Considering that B. nana on Svalbard likely descends from a Russian refugia (Alsos et al., 2007, 2015), sea ice is a reasonable transport mechanism for this location. However, genetic data from Salicaceae (S. herbacea) on Baffin Island, Greenland, and Svalbard suggest that the modern populations from these locations descend from source populations in either mid-latitude North America (south of the Laurentide Ice Sheet) or western Europe (Alsos et al., 2007, 2009), and neither region exported sea ice in the Early Holocene. This leaves wind and birds as likely mechanisms for S. herbacea transport to Baffin Island, Greenland, and Svalbard during the Holocene. North of Iceland, proxy evidence shows that sea ice disappeared by ∼11200 BP (Figure 2B, Xiao et al., 2017) when surface ocean currents were established bringing warm Atlantic waters to the North Iceland Shelf (Figure 2C, Eiríksson et al., 2000; Sha et al., 2022), nearly 500 years before the colonization of the first woody taxon on the island (Figure 2A). Thus, with the absence of sea ice around Iceland’s coastline during woody plant colonization, wind and/or birds remain the likeliest dispersal mechanisms for Iceland, similar to other Arctic regions.
As the planet continues to warm, glacier and ice sheet mass loss is accelerating (Hugonnet et al., 2021), providing new territory for woody shrubs to colonize – the Greenland Ice Sheet currently occupies 1.7 million km2 (Morlighem et al., 2017) with >400,000 km2 more covered by glaciers elsewhere in the Arctic (RGI Consortium, 2017). Global warming will also allow woody plants to migrate poleward across unglaciated Arctic tundra (e.g., Myers-Smith et al., 2011), which occupies a substantially larger area compared to glaciated regions (5 billion km2, Walker et al., 2005). In this study, we demonstrate that Salicaceae can migrate into deglaciating North Atlantic habitats rapidly whereas Betulaceae may be delayed by millennia. However, the ability of these taxa to successfully follow the rapid pace of ongoing range shifts in non-glaciated tundra (e.g., Alaska and Siberia) remains an open question (e.g., Davis and Shaw, 2001; Thuiller et al., 2009). This is partially due to different baseline conditions of the tundra ecosystem compared to glacial regions upon deglacial warming, which already contain established ecosystems and nutrient-rich soils bound in the permafrost (e.g., Heijmans et al., 2022).
While the causes and rates of present-day climate change differ from the preceding postglacial period (e.g., Tierney et al., 2020), the factors that we identify as potentially driving the colonization patterns (i.e., environmental tolerance, modes of reproduction, and soil development) are relevant in both scenarios. As a result, circum North Atlantic shrubification in deglaciating regions may be prolonged – ultimately dampening the rate of change, at least temporarily, in regional biodiversity and food web structure, as well as high-latitude temperature amplification. An important next step for constraining future shrubification rates is developing detailed paleoecological reconstructions of plant dynamics in multiple tundra regions (e.g., Clarke et al., 2020; Huang et al., 2021), continuing these efforts in glaciated regions, and pairing plant histories with independent local records of climate, such as temperature and precipitation. The latter paleoclimate reconstructions will enable more detailed assessment of plant colonization lags relative to climate (e.g., Crump et al., 2019). By pairing paleoecological and paleoenvironmental records from formerly glaciated and tundra regions and integrating these with predictive models (e.g., Braconnot et al., 2012), the community will be able to reduce uncertainties and better quantify future shrubification rates, which are essential to inform ecological predictions for policymakers.
Materials and methods
Lake sediment cores and age control
Stóra Viðarvatn (66.24°N, 15.84°W) is a large (2.4 km2), deep lake (48 m) located at 151 m asl in northeast Iceland (Figure 1, Axford et al., 2007). In winter 2020, we recovered a composite 8.93 m long sediment core (20SVID-02, Fig. S1) from 17.4 m water depth using lake ice as a coring platform. The sediment was collected in 7 drives of ∼150 cm each. The core sections were subsequently stored at 4 °C before opening for sediment subsampling. The chronology of 20SVID-02 is based on 5 marker tephra layers (volcanic ash) of known age: Askja S, G10ka Series, Kverkfjöll/Hekla, Kverkfjöll, and Hekla 4 tephra layers, with ages of 10830 ± 57 BP (Bronk Ramsey et al., 2015), 10400 to 9900 BP (Óladóttir et al., 2020), 6200 BP (Óladóttir et al., 2011), 5200 BP (Óladóttir et al., 2011) and 4200 ± 42 BP (Dugmore et al., 1995), respectively (see Supplemental Information). A Bayesian age model was generated using the R package rbacon and default settings (Blaauw and Christen, 2011) (Figure S2A).
Torfdalsvatn (66.06°N, 20.38°W) is a small (0.4 km2), shallow (5.8 m) lake located at 52 m asl in north Iceland (Figure 1, Rundgren et al., 1997). Paleovegetation records from this lake are based on pollen, macrofossil assemblages (Björck et al., 1992; Rundgren, 1995, 1998; Rundgren and Ingólfsson, 1999), and sedaDNA (Alsos et al., 2021). Sediment cores from Torfdalsvatn were also recovered by a joint Colorado-Iceland team in 2004, 2008, 2012, and 2020. In modifying the lake sediment core chronologies, we use the recently calibrated age model (Figure S2B, Geirsdóttir et al., 2020) for the pollen/macrofossil records (Björck et al., 1992; Rundgren, 1995) that uses the IntCal20 calibration curve (Reimer et al., 2020). We note that the ∼12000 BP Vedde Ash has been previously identified in this record based on major oxide composition (Björck et al., 1992). However, the age of the Vedde Ash is stratigraphically too old for the radiocarbon-based chronology, suggesting that this tephra layer likely represents ash from a separate and subsequent volcanic eruption that produced the Vedde Ash, of which several possible correlations have been identified in Iceland and mainland Europe (e.g., Pilcher et al., 2005; Kristjánsdóttir et al., 2017; Matthews et al., 2011; Geirsdóttir et al., 2022). For the sedaDNA record, the Early Holocene portion of lake sediment core (Alsos et al., 2021) features two age control points, the so-called “Saksunarvatn Ash” (10267 ± 89 BP) and a graminoid radiocarbon age (6620 ± 120 BP, Alsos et al., 2021). To better compare with the pollen/macrofossil record, we generated a new age model for the sedaDNA record (Alsos et al., 2021), using the lowermost radiocarbon age and the upper and lower limits of the main ∼20 cm thick G10ka Series/Saksunarvatn tephra unit (10400 to 9900 BP) following the recent recognition of multiple associated tephra layers and dating of these layers in Iceland (Harning et al., 2018, 2019; Óladóttir et al., 2020). The new Bayesian age model was generated using the R package rbacon and default settings (Blaauw and Christen, 2011) with the IntCal20 calibration curve (Reimer et al., 2020) (Figure S2C).
We subsampled the Stóra Viðarvatn sediment core halves immediately upon splitting in a dedicated clean lab facility with no PCR products in the Trace Metal Lab, University of Colorado Boulder. All surfaces and tools were treated with 10% bleach and 70% ethanol before use. Personnel wearing complete PPE took a total of 75 samples using clean metal spatulas. Samples were then stored in sterile Falcon tubes at 4°C until DNA extraction and metabarcoding. Sampling resolution was based upon a preliminary age model with a sample estimated every 150 years.
We performed DNA extraction and metabarcoding library generation in a dedicated ancient DNA laboratory at the Paleogenomics Lab, University of California Santa Cruz. While working in this laboratory we followed commonly used practices for minimizing contamination of ancient and degraded samples described previously (Fulton and Shapiro, 2019). We initially cleaned all instruments and surfaces using 10% bleach and 70% ethanol before UV irradiation for at least 1 hour. From each homogenized sample of the core, we aliquoted two 50 mg subsamples and isolated DNA following the column purification method described in Rohland et al. (2018) using Binding Buffer D and eluting 50 µl volumes. We pooled extraction eluates derived from the same original core sample prior to subsequent experiments and included one extraction control (containing no sample) for every 12 samples processed to assess the presence of external contaminants or cross contamination.
We performed quantitative PCR (qPCR) to determine the ideal dilution of extract to add to the metabarcoding PCR, as well as the ideal number of PCR cycles. We added 2 µl of neat or diluted extract to wells containing 1X Qiagen Multiplex master mix, as well as a final concentration of 0.16 µm of the forward and reverse trnL primer and 0.12X SYBR Green I Dye. Cycling conditions were as follows: a 15 min 95C activation, followed by 40 cycles of 94C for 30 sec, 57C for 30 sec, and 72C for 60 sec. We tested 1:10 and 1:100 dilutions of extract to water alongside neat extract. We used cycle threshold (CT) values to compare PCR efficiency between dilutions and determined the ideal cycle number for the metabarcoding PCR as the number of cycles at which the PCR curve reached the plateau phase.
We performed metabarcoding PCR on each extract using the best dilution as determined by qPCR and versions of the chloroplast trnL primer set (Taberlet et al., 2007) that contained attached truseq adapters (Table S2). We used five replicate PCRs for each primer set and for each sample, with reaction conditions as follows: 2 µl of diluted or undiluted extract in 1X Qiagen Multiplex Mix and a 0.16 µM concentration of each primer at a total volume of 25 µl. Thermocycling began with 15 min at 95C, followed by a number of cycles (as determined by qPCR) at 94C for 30 sec, 50-57C for 90 sec, 72C for 60 sec. We used one negative control PCR (no sample) alongside every 16 samples to assess the possibility of contamination during the PCR set up. Extraction controls also underwent metabarcoding. We cleaned reactions using SPRI select beads and eluted them into a final volume of 20 µl.
We then used indexing PCR to attach the full truseq adapter and unique identifier. We added 5 µl of cleaned PCR product from the metabarcoding PCR to 1X Kapa Hifi master mix and dual-unique truseq style indices at a concentration of 0.4 µM. Thermocycling began with an activation step at 98C for 3 min, followed by 8 cycles of 98C for 20 sec, 65C for 30 sec, 72C for 40 sec, and a final extension at 72C for 2 min. We cleaned reactions using SPRI select beads and quantified DNA concentration using the Nanodrop 8000 Spectrophotometer. We pooled PCR replicates from all samples in equimass ratios and sequenced the pools on an Illumina Nextseq 550 platform. Sequencing was performed using Illuminas v3 chemistry and a 150 cycle mid output sequencing kit with paired end reads of 76 base pair length.
We processed raw fastq files and performed taxonomic assignment using the Anacapa Toolkit (Curd et al., 2019) and the ArcBorBryo library, which contains 2280 sequences of Arctic and boreal vascular plants and bryophytes (Sønstebø et al., 2010). Briefly, we removed adapter sequences from the raw fastq data using cutadapt and trimmed low quality bases using FastX-toolkit before reads were separated into paired and unpaired files (Hannon, 2010; Martin, 2011). We then used dada2 for further filtering of chimeric sequences and for merging of paired reads (Callahan et al., 2016.). We dereplicated identical sequences present in the data using dada2 and generated ASV (amplicon sequence variant) tables and fastq files for each of the read types (paired merged, paired unmerged, unpaired forward, and unpaired reverse). ASV’s were assigned taxonomy by globally aligning to a CRUX database generated from the ArcBorBryo library using Bowtie2 before assignment with Anacapa’s custom BLCA algorithm (Langmead and Salzberg, 2012). Outputted taxonomy site frequency tables containing taxonomic assignments and read counts were subsequently analyzed using R. ASV sequences for Salicaceae and Betulaceae identified in our samples are included in the supplement (Table S3).
To minimize the chance of false positives, we retained only sequences that: 1) were not detected in negative controls, 2) had 100% match to sequences in the library, 3) had a minimum of 10 reads per PCR replicate and occurred in a minimum of two of five PCR replicates, and 4) had a minimum of 100 reads across all PCR replicates (Table S4). We verified non-native taxa identified by this pipeline via comparison with the NCBI nucleotide database using BLAST (http://www.ncbi.nlm.nih.gov/ blast/) and removed these due to the low likelihood of correct taxonomic assignment. Finally, DNA quality scores were calculated following Rijal et al. (2021).
Published sedaDNA datasets
To place the Icelandic plant sedaDNA datasets in the context of the circum North Atlantic, we compiled all existing lake sedaDNA records of vascular plants from this region that were formally glaciated during the Last Glacial Maximum (Figure 1, Table 1). These include records from Baffin Island (n=1, Crump et al., 2019), northern Greenland (n=1, Epp et al., 2015), Svalbard (n=2, Alsos et al., 2016; Volstad et al., 2020), northern Norway (n=3, Rijal et al., 2021; Alsos et al., 2022), and southern Norway (n=1, ter Schure et al., 2021). To ensure that all records were comparable, we excluded 7 records from northern Norway: Sandfjorddalen, Horntjernet, Gauptjern, Jøkelvatnet, Kuutsjärvi, Nesservatnet, Sierravannet (Rijal et al., 2021; Alsos et al., 2022). Nesservatnet and Sierravannet contained only Late Holocene records and thus do not capture local postglacial colonization patterns. A lack of supporting proxy records (e.g., carbon content) for Sandfjorddalen and Horntjernet meant it was unclear whether these two records capture the complete postglacial period. For Gauptjern, the inferred basal age (8776 BP) is inconsistent with the reconstructed timing of deglaciation (∼11000 BP, Hughes et al., 2016), which suggests the initial postglacial interval may be missing. Finally, both Jøkelvatnet and Kuutsjärvi were impacted by glacial meltwater during the Early Holocene when woody taxa are first identified (Wittmeier et al., 2015; Bogren, 2019), and thus the inferred timing of plant colonization is probably confounded in this unstable landscape by periodic pulses of terrestrial detritus. Unless otherwise specified, the timing of deglaciation at each site is inferred from the basal age of the sediment record.
This study was supported by NSF ARCSS #1836981. We kindly thank Sveinbjörn Steinþórsson and Þór Blöndahl for lake coring assistance at Stóra Viðarvatn, Mats Rundgren for sharing Torfdalsvatn’s pollen datasets, Thomas Marchitto for access to the Trace Metal Lab at the University of Colorado Boulder, and Martha Raynolds and Helga Bültmann for valuable discussion. Publication of this article was funded by the University of Colorado Boulder Libraries Open Access Fund. We thank two anonymous reviewers for their constructive comments that improved the final version of our manuscript.
GHM, ÁG and JS conceptualized funded the project; GHM, BS and ÁG supervised the project; GHM, ÁG, DJH and JHR cored the lake; DJH and NA sampled the sediment; TT performed tephra analyses and identification; SS performed DNA extraction and library generation; DJH performed taxononmic assignment and processed the data, constructed age models, and compiled published datasets; DJH wrote the paper with contributions and expertise from SS, KAJ, NA, TT, JS, ÁG, BS and GHM.
The authors declare that they have no competing interests.
- 1.Past and future range shift and loss of genetic diversity in dwarf willow (Salix herbacea L.) inferred from genetics, fossils, and modellingGlobal Ecology and Biogeography 18:223–239
- 2.Long-distance plant dispersal to North Atlantic islands: colonization routes and founder effectAoB PLANTS 7:1–19
- 3.The role of sea ice for vascular plant dispersal in the ArcticBiology Letters 12
- 4.Plant DNA metabarcoding of lake sediment: How does it represent the contemporary vegetationPLoS ONE 13:1–23
- 5.Ancient sedimentary DNA shows rapid post-glacial colonisation of Iceland followed by relatively stable vegetation until the Norse settlement (Landnám) AD 870Quaternary Science Reviews 259
- 6.Postglacial species arrival and diversity buildup of northern ecosystems took millenniaScience Advances 8:1–13
- 7.Sedimentary ancient DNA from Lake SkartjørnaSvalbard: Assessing the resilience of arctic flora to Holocene climate change. The Holocene 26:627–642
- 8.Betula pendula Roth (B. verrucosa Ehrhand B. pubescens Ehrh. Journal of Ecology 80:837–870
- 9.Holocene temperature history of northern Iceland inferred from subfossil midgesQuaternary Science Reviews 26:3344–3358
- 10.The configuration of Northern Hemisphere ice sheets through the QuaternaryNature Communications 10:1–10
- 11.Salix herbacea LJournal of Ecology 86:872–895
- 12.Contributions of Quaternary botany to modern ecology and biogeographyPlant Ecology and Diversity 12:189–385
- 13.The importance of plant macrofossils in the reconstruction of Lateglacial vegetation and climate: examples from Scotland, western Norway, and Minnesota, USAQuaternary Science Reviews 22:453–473
- 14.Lake Torfadalsvatn: a high resolution record of the North Atlantic ash zone I and the last glacial-interglacial environmental changes in IcelandBoreas 21:15–22
- 15.Flexible paleoclimate age-depth models using an autoregressive gamma processBayesian Analysis 6:457–474
- 16.Evidence for birch forests and a highly productive environment near the margin of the Fennoscandian ice sheet in the Värriötunturit area, northeastern FinlandMSc thesis, Stockholm University, 41 pp
- 17.Evaluation of climate models using palaeoclimatic dataNature Climate Change 2:417–424
- 18.Improved age estimates for key Late Quaternary European tephra horizons in the RESET latticeQuaternary Science Reviews 118:18–32
- 19.Plant succession and soil development on the foreland of the Morteratsch glacier (Pontresina, Switzerland): straight forward or chaotic?Flora 205:561–576
- 20.Early Holocene climate variability and the timing and extent of the Holocene thermal maximum (HTM) in northern IcelandQuaternary Science Reviews 25:2314–2331
- 21.DADA2: High-resolution sample inference from Illumina amplicon dataNature Methods 13:581–583
- 22.Lake sedimentary DNA research on past terrestrial and aquatic biodiversity: Overview and recommendationsQuaternary 4:1–61
- 23.A 24,000-year ancient DNA and pollen record from the Polar Urals reveals temporal dynamics of arctic and boreal plant communitiesQuaternary Science Reviews 247
- 24.Shrub range expansion alters diversity and distribution of soil fungal communities across an alpine elevation gradientMolecular Ecology 27:2461–2476
- 25.Ancient plant DNA reveals High Arctic greening during the Last InterglacialProceedings of the National Academy of Sciences 118:1–9
- 26.Arctic shrub colonization lagged peak postglacial warmth: Molecular evidence in lake sediment from Arctic CanadaGlobal Change Biology 25:4244–4256
- 27.Anacapa Toolkit: An environmental DNA toolkit for processing multilocus metabarcode datasetsMethods in Ecology and Evolution 10:1469–1475
- 28.Range shifts and adaptive responses to Quaternary climate changeScience 292:673–679
- 29.Betula nana L. and Betula glandulosa MichxJournal of Ecology 85:241–264
- 30.A Lakeland Area in the Late Miocene. In: Late Cainozoic Floras of Iceland. Topics in Geobiology, vol 35
- 31.The Pleistocene Floras (2.4–0.8 Ma) – Shaping the Modern Vegetation of Iceland. In: Late Cainozoic Floras of Iceland. Topics in Geobiology, vol 35
- 32.Radiocarbon dating tephra layers from Britain and IcelandRadiocarbon 37:379–388
- 33.Plot-scale evidence of tundra vegetation change and links to recent summer warmingNature Climate Change 2:8–12
- 34.Lake sediment multi taxon DNA from North Greenland records early post-glacial appearance of vascular plants and accurately tracks environmental changesQuaternary Science Reviews 117:152–163
- 35.Life on the periphery is tough: Vegetation in Northwest Iceland and its responses to early-Holocene warmth and later climate fluctuationsThe Holocene 25:1437–1453
- 36.Comparative analyses of plastid and AFLP data suggest different colonization history and asymmetric hybridization between Betula pubescens and B. nanaMolecular Ecology 24:3993–4009
- 37.Late-glacial and Holocene palaeoceanography of the North Icelandic shelfJournal of Quaternary Science 15:23–42
- 38.Arctic greening from warming promotes declines in caribou populationsScience Advances 3:1–9
- 39.Multi-proxy reconstructions of Holocene environmental change and catchment biogeochemistry using algal pigments and stable isotopes preserved in lake sediment from Baffin Island and Iceland. PhD Dissertation, University of Colorado Boulder
- 40.Setting Up an Ancient DNA LaboratoryAncient DNA. Methods in Molecular Biology, vol
- 41.Holocene history of landscape instability in Iceland: Can we deconvolve the impacts of climate, volcanism and human activity?Quaternary Science Reviews 249
- 42.Recurrent outburst floods and explosive volcanism during the Younger Dryas-Early Holocene deglaciation in south Iceland: evidence from a lacustrine recordJournal of Quaternary Science 37:1006–1023
- 43.Successional trends and processes on a glacial foreland in Southern Iceland studied by repeated species countsEcological Processes 8
- 45.Marker tephra in Haukadalsvatn lake sediment: A key to the Holocene tephra stratigraphy of northwest IcelandQuaternary Science Reviews 219:154–170
- 46.Provenance, stratigraphy and chronology of Holocene tephra from Vestfirðir, IcelandQuaternary Geochronology 46:59–76
- 47.Tundra vegetation change and impacts on permafrostNature Reviews Earth & Environment 3:68–84
- 48.From mountain top to the deep sea – deglaciation in 4D of the northwestern Barents Sea ice sheetQuaternary Science Reviews 75:78–99
- 49.Plant sedimentary ancient DNA from Far East Russia covering the last 28,000 years reveals different assembly rules in cold and warm climatesFrontiers in Ecology and Evolution 9
- 50.The last Eurasian ice sheets – a chronological database and time-slice reconstructionDATED-1 Boreas 45:1–45
- 51.Accelerated global glacier mass loss in the early twenty-first centuryNature 592:726–731
- 52.Flandrian pollen diagrams from SvalbardGeografiska Annaler 52:213–222
- 53.Geochemical identification of potential DNA-hotspots and DNA-infrared fingerprints in lake sedimentsApplied Geochemistry 122
- 54.Birch hybridization in ThistilfjördurNorth-east Iceland during the Holocene. Icelandic Agricultural Sciences 27:95–109
- 55.Thórsson ÆTh, Hallsdóttir M, Sigurgeirsson, Eysteinsson Th, Anamthawat - Jónsson KDifferentiating pollen of Betula species from Iceland. Grana 4 :78–84
- 56.Íslenskt plöntutal, blómplöntur og byrkningarFjölrit Náttúrufræðistofnunar 51:1–58
- 57.Geochemistry of Holocene cryptotephras from the North Iceland Shelf (MD99-2269): intercalibration with radiocarbon and palaeomagnetic chronostratigraphiesThe Holocene 17:155–176
- 58.The Late Weichselian and Holocene shoreline displacement on the west-central coast of SvalbardPolar Research 5:29–44
- 59.Fast gapped-read alignment with Bowtie 2Nature Methods 9:357–359
- 60.Developments in plant colonization and succession on Surtsey during 1999-2008Surtsey Research 12:57–76
- 61.Cutadapt removes adapter sequences from high-throughput sequencing readsEMBnet.journal 17:10–12
- 62.New age estimates and climatostratigraphic correlations for the Borrobol and Penifiler Tephras: evidence from Abernethy ForestScotland. Journal of Quaternary Science 26:247–252
- 63.BedMachine v3: Complete bed topography and ocean bathymetry mapping of Greenland from multibeam echo sounding combined with mass conservationGeophysical Research Letters 44:51–11
- 64.Shrub expansion in tundra ecosystems: dynamics, impacts and research prioritiesEnvironmental Research Letters 6:1–15
- 65.Long-distance dispersal of plantsScience 313:786–788
- 66.Minimizing polymerase biases in metabarcodingMolecular Ecology Resources 18:927–939
- 67.Holocene volcanic activity at Grímsvötn, Bárdarbunga, and Kverkfjöll subglacial centres beneath Vatnajökull, IcelandBulletin of Volcanology 73:1187–1208
- 68.The Saksunarvatn Ash and the G10ka series tephra. Review and current state of knowledgeQuaternary Geochronology 56
- 69.High-Arctic climate conditions for the last 7000 years inferred from multi-proxy analysis of the Bliss Lake recordNorth Greeland. Journal of Quaternary Science 27:318–327
- 70.Rekonstruksjon av skredaktiviteten rundt Langfjordvatnet på Arnøy, Nord-TromsMSc Thesis, University of Bergen
- 71.Shifts in Arctic vegetation and associated feedbacks under climate changeNature Climate Change 3:673–677
- 72.Seasonal sea ice persisted through the Holocene Thermal Maximum at 80°NCommunications Earth & Environment 2:1–10
- 73.A Holocene tephra record from the Lofoten IslandsArctic Norway. Boreas 34:136–156
- 74.The Arctic has warmed nearly four times faster than the globe since 1979Communications Earth & Environment 3
- 75.A new Greenland ice core chronology for the last glacial terminationJournal of Geophysical Research 111:1–16
- 76.The IntCal20 northern hemisphere radiocarbon age calibration curve (0-55 cal kBP)Radiocarbon 62:725–757
- 77.Randolph Glacier Inventory (RGI) – A Dataset of Global Glacier Outlines: Version 6.0. Technical Report, Global Land Ice Measurements from Space, Boulder, Colorado, USA. Digital Media
- 78.Sedimentary ancient DNA shows terrestrial plant richness continuously increased over the Holocene in northern FennoscandiaScience Advances 7:1–16
- 79.Extraction of highly degraded DNA from ancient bones, teeth and sediments for high-throughout sequencingNature Protocols 13:2447–2461
- 80.Biostratigraphic evidence of the Allerød-Younger Dryas-Preboreal Oscillation in Northern IcelandQuaternary Research 44:405–416
- 81.Early Holocene vegetation of northern Iceland: pollen and plant macrofossil evidence from the Skagi peninsulaThe Holocene 5:553–564
- 82.Plant survival in Iceland during periods of glaciation?Journal of Biogeography 26:387–396
- 83.Consistently dated records from the Greenland GRIP, GISP2, and NGRIP ice cores for the past 104 ka reveal regional millennial-scale δ18O gradients with possible Heinrich event imprintQuaternary Science Reviews 106 :29–46
- 84.Diatom-reconstructed summer sea-surface temperatures and climatic events off Norh Iceland during the last deglaciation and HolocenePalaeogeography, Palaeoclimatology, Palaeoecology 602
- 85.Lake sedimentary DNA accurately records 20th Century introductions of exotic conifers in ScotlandNew Phytologist 213:929–941
- 86.Using next-generation sequencing for molecular reconstruction of past Arctic vegetation and climateMolecular Ecology Resources 10:1009–1018
- 87.Changing snow and shrub conditions affect albedo with global implicationsJournal of Geophysical Research 110:1–13
- 88.Greater deciduous shrub abundance extends tundra peak season and increases modeled net CO2 uptakeGlobal Change Biology 21:2394–2409
- 89.Holocene changes in sea-ice cover and polynya formation along the eastern North Greenland shelf: New insights from biomarker recordsQuaternary Science Reviews 231
- 90.Spatial and temporal dynamics of growth of woody plant species (birch and willows) on the foreland of a retreating glacier in southern IcelandEcological Processes 10:1–11
- 91.Power and limitations of the chloroplast trnL (UAA) intron for plant DNA barcodingNucleic Acids Research 35
- 92.The evidence for shrub expansion in Northern Alaska and the Pan-ArcticGlobal Change Biology 12:686–702
- 93.Anthropogenic and environmental drivers of vegetation change in southeastern Norway during the HoloceneQuaternary Science Reviews 270
- 94.Introgression and phylogeography of Betula nana (diploid)B. pubescens (tetraploid) and their triploid hybrids in Iceland inferred from cp-DNA haplotype variation. Journal of Biogeography 37:2098–2110
- 95.Predicting global change impacts on plant species’ distributions: Future challengesPerspectives in Plant Ecology, Evolution and Systematics 9:137–152
- 96.Past climates inform our futureScience 370:1–9
- 97.Northern Hemisphere vegetation change drives a Holocene thermal maximumScience Advances 8:1–10
- 98.Early stage development of selected soil properties along the proglacial moraines of Skaftafellsjökull glacier, SE-IcelandCatena 121:142–150
- 99.A complete Holocene lake sediment ancient DNA record reveals long-standing high Arctic plant diversity hotspot in northern SvalbardQuaternary Science Reviews 234
- 100.Talbot SS, Yurtsev BA, other members of the CAVM TeamThe circumpolar Arctic vegetation map. Journal of Vegetation Science 16 :267–282
- 101.Plant population patterns in a glacier foreland succession: pioneer herbs and later-colonizing shrubsEcography 16:117–136
- 102.Reconstructing Holocene glacier activity at Langfjordjøkelen, Arctic Norway, using multi-proxy fingerprinting of distal glacier-fed lake sedimentsQuaternary Science Reviews 114:78–99
- 103.Deglacial and Holocene sea-ice variability north of Iceland and response to ocean circulation changesEarth and Planetary Science Letters 472:14–24