Cutting Edge: Changing the rules of the game

  1. Dan MacLean  Is a corresponding author
  1. Sainsbury Laboratory, United Kingdom
1 figure


Fraxinus in Facebook.

The top screenshot shows the start of the game. The target pattern is at the top of the screen and the aim of the game is to align the read patterns with this target. The target pattern is always a 21-base stretch of DNA from a reference genome that is likely to contain a genetic variant; in Fraxinus we are starting with targets taken from a reference genome for C. fraxinea, the pathogen that causes ash dieback disease, but the targets can be chosen from any reference genome. The read patterns used in the game can contain up to 76 bases and, in this case, are taken from samples of an interesting strain of C. fraxinea. The arrow buttons allow each read pattern to be moved left or right, while individual leaves (bases) can be moved, deleted or inserted with click-and-drag movements. The pattern value at the bottom of the screen indicates the player’s score. The bottom screenshot shows the game after the player has shifted the top two reads 14 bases to the left to match the seven bases on the left of the target, and then introduced a 9-base insertion (black circles) to match another three bases towards the right of the target: these moves increase the pattern value to −80. (Note that zero is not the maximum possible score.)

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  1. Dan MacLean
Cutting Edge: Changing the rules of the game
eLife 2:e01294.