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Following exposure of Leishmania culture promastigotes to DNA stress, single-cell RNA sequencing reveals discrete clusters of cells associated with efficient generation of hybrids between different species and strains in vitro marked by expression of the ancestral gamete fusogen HAP2.
The Arabidopsis N6-methyladenosine (m6A) binding proteins ECT2 and ECT3 act redundantly in the cytoplasm to regulate m6A-containing mRNAs whose abundances are reduced in ect2/ect3/ect4 knockout plants although the locations of their polyadenylation sites remain unaffected, contrary to currently held beliefs.
The Arabidopsis N6-methyladenosine (m6A)-binding protein ECT2 binds to m6A in the canonical RR[m6A]CH motif, not a plant-specific motif as previously thought, and its intrinsically disordered region contributes to mRNA binding via contacts with U-rich motifs.
Using genetics, it is possible to uncouple higher adiposity from its adverse metabolic effects and show that some obesity-associated conditions may benefit from treating the metabolic effects alone, whilst others may benefit more from weight loss.