1. Neuroscience
Download icon

Retraction: Protein kinase C is a calcium sensor for presynaptic short-term plasticity

Retraction
  • Cited 0
  • Views 3,703
  • Annotations
Cite this article as: eLife 2018;7:e35974 doi: 10.7554/eLife.35974

Main text

Fioravante D, Chu YX, de Jong APH, Leitges M, Kaeser PS, Regehr WG. 2014. Protein kinase C is a calcium sensor for presynaptic short-term plasticity. eLife 3:e03011. doi: 10.7554/eLife.03011.

Published 5, August 2014

The authors are retracting the eLife paper cited above based on irregularities associated with the electrophysiology experiments performed by YXC that were discovered by the corresponding author (WGR). The most important experiments are those in which the PKCβ is virally expressed in presynaptic cells in PKCαβ dko animals, in which the calcium sensitive PKC isoforms are eliminated in presynaptic cells and post-tetanic potentiation (PTP) is very small. In some cases the calcium sensitive wildtype, PKCβWT, is expressed and PTP is rescued (Figure 1F), and in other cases mutated calcium-insensitive PKCβD/A is expressed and PTP is not rescued (Figure 3B).

A careful re-examination of these experiments revealed that for many cells in PKCβWT rescue experiments, synaptic enhancement was accompanied by an increase in the stimulus artifact and a speeding of excitatory postsynaptic current (EPSC) decays. Both of these effects were transient and had approximately the same time course as the enhancement of synaptic amplitude. The alterations in artifact and time course were not present in experiments in which PKCβD/A was expressed. No biological mechanism has been identified that can account for these changes in stimulus artifact and EPSC decay. For each PTP trial, data were collected continuously for approximately two minutes, and there was no independent measure of series resistance and series resistance compensation throughout the trial. It was assumed that changes in series resistance would be small, and that series resistance compensation would remain constant throughout each trial. These challenging experiments will need to be repeated in a verifiably blind manner.

The authors will post an update if further information becomes available in the future, but in light of the re-analysis the key conclusions of this work are now in doubt and the authors agree that the paper should be retracted.

Article and author information

Author details

  1. Diasynou Fioravante

    Contributed equally with
    YunXiang Chu
  2. YunXiang Chu

    Contributed equally with
    Diasynou Fioravante
  3. Arthur PH de Jong

  4. Michael Leitges

  5. Pascal S Kaeser

  6. Wade G Regehr

    For correspondence
    wade_regehr@hms.harvard.edu

Publication history

  1. Version of Record published: March 7, 2018 (version 1)

Copyright

© 2018, Fioravante et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 3,703
    Page views
  • 0
    Citations

Article citation count generated by polling the highest count across the following sources: Crossref, PubMed Central, Scopus.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Download citations (links to download the citations from this article in formats compatible with various reference manager tools)

Open citations (links to open the citations from this article in various online reference manager services)