1 figure


(A) Proliferation of MDA-MB-231, subjected to sgROSA26 (g1) or sgMELK (g6).

Cas9-expressing MDA-MB-231 cells were seeded into 24-well plates at 250 or 25,000 cells per well, and imaged 11 or 3 days after seeding, respectively (Celigo Image Cytometry, Nexcelom Biosciences). Whole well images are shown with green pseudo coloring to visualize cell confluence. Graphs indicate mean ± SD (n = 4). Cas9-expressing MDA-MB-231 and lentiGuide constructs were generous gifts of Dr. Sheltzer. (B) Proliferation of MDA-MB-468 wild-type parental cells, MELK wild-type (E9) and MELK-knockout (E3, C7, and C9) clonal sub-lines under clonogenic growth conditions. Anti-MELK immunoblotting was visualized using the Odyssey CLx infrared imaging system (LI-COR Biosciences).

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Yubao Wang
  2. Young-Mi Li
  3. Lukas Baitsch
  4. Alan Huang
  5. Yi Xiang
  6. Haoxuan Tong
  7. Ana Lako
  8. Thanh Von
  9. Christine Choi
  10. Elgene Lim
  11. Junxia Min
  12. Li Li
  13. Frank Stegmeier
  14. Robert Schlegel
  15. Michael J Eck
  16. Nathanael S Gray
  17. Timothy J Mitchison
  18. Jean J Zhao
Correction: MELK is an oncogenic kinase essential for mitotic progression in basal-like breast cancer cells
eLife 7:e36414.