1. Cell Biology
Download icon

Correction: MELK is an oncogenic kinase essential for mitotic progression in basal-like breast cancer cells

Cite this article as: eLife 2018;7:e36414 doi: 10.7554/eLife.36414
1 figure


(A) Proliferation of MDA-MB-231, subjected to sgROSA26 (g1) or sgMELK (g6).

Cas9-expressing MDA-MB-231 cells were seeded into 24-well plates at 250 or 25,000 cells per well, and imaged 11 or 3 days after seeding, respectively (Celigo Image Cytometry, Nexcelom Biosciences). Whole well images are shown with green pseudo coloring to visualize cell confluence. Graphs indicate mean ± SD (n = 4). Cas9-expressing MDA-MB-231 and lentiGuide constructs were generous gifts of Dr. Sheltzer. (B) Proliferation of MDA-MB-468 wild-type parental cells, MELK wild-type (E9) and MELK-knockout (E3, C7, and C9) clonal sub-lines under clonogenic growth conditions. Anti-MELK immunoblotting was visualized using the Odyssey CLx infrared imaging system (LI-COR Biosciences).

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Download citations (links to download the citations from this article in formats compatible with various reference manager tools)

Open citations (links to open the citations from this article in various online reference manager services)