Listeria monocytogenes (purple). Image credit: Balasubr Swaminathan, Peggy Hayes, USCDCP (CC0)
Eating food that has been contaminated with bacteria called Listeria monocytogenes can result in life-threatening infections. The bacteria first invade the epithelial cells that line the small intestine. After this, L. monocytogenes can move from one host cell to another, which allows the infection to reach other organs.
Most studies into how L. monocytogenes infections spread have focused either on how single bacterial cells move from one host cell to the next, or on how millions of bacteria damage host tissues. Little was known about the intermediate steps of an infection, where the bacteria start to colonize the small intestine.
To investigate, Ortega et al. recorded videos of L. monocytogenes spreading between epithelial cells grown on a glass coverslip, and developed computer simulations to try to reproduce how the bacteria spread. This revealed that the bacteria do not all move in the same way. Instead, less than 1% of the bacteria move in ‘steps’ that are up to 10 times longer than those taken by the others. Ortega et al. named these bacteria ‘pioneers’.
Ortega et al. propose that the pioneers form long protrusions that allow them to spread directly from an infected cell to a non-neighboring cell. By taking these large steps, the pioneers may increase the chances that the bacteria will cause a long-lasting infection.
Future research will be needed to answer further questions about the pioneers. For example, how do the pioneer bacteria differ from the majority of bacterial cells? Would targeting anti-bacterial treatments at pioneers make it easier to treat infections? It also remains to be seen if other types of bacteria also show this pioneer behavior.
