The structure of the Hantaan virus nucleocapsid when it is bound to the viral genome. Image credit: Malet et al. (CC BY 4.0)
Rats and mice sometimes transmit hantaviruses, a family of microbes that can cause deadly human diseases. For example, the Hantaan virus leads to haemorrhagic fevers that are potentially fatal. There are no vaccine or even drugs against these infections.
To multiply, viruses must insert their genetic material inside a cell. While the body often detects and destroys foreign genetic information, hantaviruses can still evade our defences. Molecules called nucleoproteins bind to the viral genome, hiding it away in long helices called nucleocapsids. When the virus needs to replicate, an enzyme opens up the nucleocapsid, reads and copies the genetic code, and then closes the helix. Yet, researchers know little about the details of this process, or even the structure of the nucleocapsid.
Here, Arragain et al. use a method called cryo-electron microscopy to examine and piece together the exact 3D structure of the Hantaan virus nucleocapsid. This was possible because the new technique allows scientists to observe biological molecules at an unprecedented, near atomic resolution. The resulting model reveals that the viral genome nests into a groove inside the nucleocapsid. It also shows that specific interactions between nucleoproteins stabilise the helix. Finally, the model helps to provide hypotheses on how the enzyme could read the genome without breaking the capsid.
Mapping out the structure and the interactions of the nucleocapsid is the first step towards finding molecules that could destabilise the helix and neutralise the virus: this could help fight both the Hantaan virus and other members of its deadly family.
