Phylogeny of the 807 species including ray-finned fishes (Actinopteri), birds (Aves), insects (Insecta), mammals (Mammalia), and molluscs (Mollusca) with bars corresponding to TE content (blue), genome size (green), and dN/dS estimations (yellow). The tree was plotted with iTOL (Letunic and Bork 2021).

(A) Relationship between overall TE content and genome size (log-transformed). Slope = 0.718, adjusted-R2 = 0.751, p-value < 0.001. (B) Relationship between genome size and dN/dS. Slope = 6.100, adjusted-R2 = 0.275, p-value < 0.001. (C) Relationship between TE content and dN/dS. Slope = 4.253, adjusted-R2 = 0.092, p-value < 0.001. Statistics refer to linear regression, see Tables 1 and 2 for Phylogenetic Independent Contrasts results.

Correlation between genome size and overall TE content based on Phylogenetic Independent Contrasts. Statistics are shown relative to the overall dataset and to each clade. Variables were log-transformed previous to regression. * 0.05 < p ≤ 0.01; ** 0.01 < p ≤ 0.001; *** p < 0.001.

PIC results for the correlations of LHTs, genome size, and TE content against dN/dS. Results for Bio++ dN/dS are shown for the full dataset and for the phylogeny deprived of the longest (> 1) and shortest (< 0.01) terminal branches. Results for Coevol dN/dS are relative to the GC3-poor geneset. Only body mass and longevity are reported as LHTs (for an overview of all traits, see Table 3). For genomic traits, statistics are reported relative to the overall dataset and to each clade. Expected significant correlations are marked in bold black; significant correlations opposite to the expected trend are marked in bold red. * 0.05 < p ≤ 0.01; ** 0.01 < p ≤ 0.001; *** p < 0.001

Correlation coefficients (CC) and posterior probabilities (PP) estimated by Coevol with the GC3-poor and GC3-rich genesets for the coevolution of dN/dS with life history and genomic traits. Different LHTs are shown according to availability for a clade. Posterior probabilities lower than 0.1 indicate significant negative correlations; posterior probabilities higher than 0.9 indicate significant positive correlations. Expected significant correlations are marked in bold black; significant correlations opposite to the expected trend are marked in bold red. aPantheria, bAnAge.