Abstract
A high density of tumor-associated macrophages (TAMs) is associated with poorer prognosis and survival in breast cancer patients. Recent studies have shown that lipid accumulation in TAMs can promote tumor growth and metastasis in various models. However, the specific molecular mechanisms that drive lipid accumulation and tumor progression in TAMs remain largely unknown. Herein, we demonstrated that unsaturated fatty acids (FAs), unlike saturated ones, are more likely to form lipid droplets in macrophages. Specifically, unsaturated FAs, including linoleic acids (LA), activate the FABP4/CEBPα pathway, leading to triglyceride synthesis and lipid droplet formation. Furthermore, FABP4 enhances lipolysis and FA utilization by breast cancer cells, which promotes cancer cell migration in vitro and metastasis in vivo. Notably, a deficiency of FABP4 in macrophages significantly reduces LA-induced lipid metabolism. Therefore, our findings suggest FABP4 as a crucial lipid messenger that facilitates unsaturated FA-mediated lipid accumulation and lipolysis in TAMs, thus contributing to the metastasis of breast cancer.
Graphic Abstract
Highlights
Unlike saturated fatty acids, unsaturated fatty acids preferentially promote lipid droplet formation in macrophages.
Unsaturated fatty acids activate the FABP4/CEBPα axis for neutral lipid biosynthesis in macrophages
Deficiency of FABP4 compromised unsaturated fatty acid-mediated lipid accumulation and utilization in macrophages
FABP4-mediated lipid metabolism in macrophages contributes to breast cancer metastasis
eLife Assessment
This important paper uses elegant models, including genetic knock outs, to demonstrate that FABP4 contributes to lipid accumulation in tumor-associated macrophages, which seems to increase breast cancer migration. While the work is of high interest, the strength of the evidence relating to some of the conclusions is incomplete and the paper would benefit from some refinement. The work will be of interest to those researchers trying to link metabolism, the immune system, and cancer.
Significance of findings
important: Findings that have theoretical or practical implications beyond a single subfield
- landmark
- fundamental
- important
- valuable
- useful
Strength of evidence
incomplete: Main claims are only partially supported
- exceptional
- compelling
- convincing
- solid
- incomplete
- inadequate
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Introduction
As a main arm of innate immunity, macrophages are present in almost all tissues of an organism, where they play pivotal roles in maintaining tissue homeostasis or contributing to disease pathogenesis1. In breast cancer, tumor associated macrophages (TAMs) are the most abundant immune cells in the tumor microenvironment (TME), actively participating in tumor progression and metastasis2–4. A high presence of TAMs in the TME correlates with a poorer prognosis of cancer patients in clinical studies5–7. Moreover, macrophage deletion via genetic or therapeutic approaches results in inhibition and regression of mammary tumors in pre-clinical studies8–10. These ample lines of evidence corroborate a crucial role for macrophages in facilitating breast cancer progression.
Macrophages are known for their heterogeneity regarding origin, phenotypes, and functional states11. In response to diverse environmental stimuli from the environment, macrophages are believed to acquire a spectrum of functional states in vivo 12,13. To understand their functional versatility, macrophages are commonly divided into the M1/M2 paradigm based on the concept of adaptive Th1/Th2 polarization14,15. Generally, Th1-derived cytokines polarize macrophages to M1 phenotype, which express inducible nitric oxide synthase and exhibit anti-tumor activities. Th2-cytokines polarized M2 macrophages highly express arginase 1, CD206, VEGFs, IL-10, TGFβ, etc., supporting angiogenesis and favoring tumor growth16,17. However, given the complex functional states of macrophages in vivo 18, the M1/M2 classification is likely oversimplified. Many studies demonstrate that TAMs do not follow the two extreme M1/M2 activation states in vivo 19,20. Considering that various extrinsic and intrinsic factors in the TME can cooperatively shape TAM phenotypes and functions21,22, identification of these factors is essential for targeting TAM-mediated cancer immunotherapy.
There is a growing realization that macrophage metabolism determines their functional outcome23,24. Several recent studies in multiple cancer models, including breast cancer25–28, reported that lipid accumulation in TAMs promotes tumor growth and metastasis. Specifically, lipid accumulation promoted the expression of Arg1, VEGFα, MMP9, IL-6, PD-L1, CCL-6, etc., thus conferring TAMs with immune suppressive and pro-tumorigenic phenotypes. Lipid droplet formation was as a characteristic feature of the pro-tumor TAMs, but the underlying molecular mechanisms driving lipid droplet formation in TAMs remain to be determined.
Despite morphological and compositional differences, lipid droplets are all formed by a phospholipid monolayer enclosing a neutral lipid core 29. A key step of lipid droplet formation is to synthesize neutral triacylglycerol (TAG), which requires the esterification of activated fatty acids (FA) to diacylglycerol (DAG)30. During this step, enzymes including glycerol-3-phosphate acyltransferase (GPAT), acyl-CoA:1-acyl-glycerol-3-phosphate acyltransferase (AGPAT), DAG acyltransferases (DGATs), are key in the biogenesis of neutral lipids. As such, factors engaging with FA transport and regulating key enzyme activity are deemed to be critical for lipid droplet formation in TAMs.
Due to the insolubility of hydrophobic FAs, fatty acid binding proteins (FABPs) have evolved to solubilize FAs, facilitating their transport and responses31. Among FABPs, adipose FABP (A-FABP or FABP4) is the most well studied given its striking biology in regulating macrophage lipid metabolism and functions in multiple disease settings1,32. Our pre-clinical studies demonstrated that a subset of macrophages with the phenotype of CD11b+F4/80+Ly6C−CD11c−MHCII− highly express FABP4, facilitating macrophage lipid processing and patrolling functions1,33. Moreover, FABP4 was identified as a functional marker for protumor TAMs by enhancing the oncogenic IL-6/STAT3 signaling19. Given the emerging role of lipid accumulation in protumor TAMs, we hypothesized that FABP4 might function as a key molecular sensor promoting lipid accumulation and utilization in TAMs.
Utilizing a combination of in vitro cellular studies with various species of FAs, in vivo macrophage single-cell sequencing in mouse models, and macrophage/FABP4 analysis in human breast cancer specimens, we demonstrate that FABP4 plays a pivotal role in mediating lipid droplet formation and subsequent lipolysis for tumor utilization, thus contributing to TAM-mediated tumor growth and metastasis in breast cancer.
Results
Unsaturated FAs promote lipid accumulation in macrophages
Hostile TME, especially in breast cancer, is characterized by enriched FAs and other lipid species, which foster immunosuppression and support tumor growth and metastasis34,35. Since palmitic acid (PA, 16:0), stearic acid (SA, 18:0), oleic acid (OA, 18:1), and linoleic acid (LA, 18:2) are the most common FAs in humans, as shown in Figure 1A, we treated macrophages with individual albumin-conjugated FAs and determined their fate. Using a macrophage cell line, we demonstrated that all the FAs studied were oxidized by mitochondria as evidenced by the increased oxygen consumption rate (OCR) when compared to the bovine serum albumin (BSA) control (Figure 1B). Interestingly, when measuring their impact on other cellular organelles, FAs exhibited dichotomous effects, with saturated FAs increasing lysosome contents (Figure 1C), while unsaturated FAs promoting ER (Figure 1D) and, more dramatically, lipid droplet (LD) formation (Figure 1E) in macrophages. Using primary peritoneal macrophages (Figure S1A) and bone marrow-derived macrophages (Figure S1B), we observed the similar phenomenon that unlike saturated FA, unsaturated FA significantly induced LD formation in these macrophages. Oil Red O staining confirmed the presence of LD formation (Figure S1C). Of note, the observation of unsaturated FA-induced LD formation in macrophages was consistent regardless of FA concentrations (Figure S1D) and treatment durations (Figure S1E). With a multispectral imaging analysis, we demonstrated that OA/LA-induced LD accumulation was not associated with the lysosome, but colocalized in the ER (Figure 1F). Transmission electron microscope further confirmed that OA and LA specifically induced LD formation in the cytoplasm (Figure 1G), which were bud from ER (Figure S1F). Collectively, these data suggest that unsaturated FA-induced LD biogenesis in macrophages mainly occurs in the ER.
Unsaturated FAs upregulate key enzymes for LD biogenesis
It is well known that key enzymes, including GPATs, AGPATs, LIPINs, DGATs, are involved in TAG synthesis and LD biogenesis (Figure 2A). To determine how unsaturated FAs, but not saturated FAs, induced LD biogenesis in macrophages, we treated macrophages with either LA or PA, and measured their impact on LD biogenesis-related enzymes. In response to PA or LA treatment, Acs1, Cpt1a, Cpt1b, HMGCR, Acat1 and Acat2 had similar expression pattern in macrophages (Figure S2A-S2F), suggesting that both FA types exerted similar effects on FA activation, mitochondrial oxidation, cholesterol synthesis and esterification. Interestingly, genes encoding rate-limiting enzymes in TAG synthesis, including Gpam1, Dgat1 and Dgat2, were significantly increased by treatment with LA, but not PA (Figure 2B-2H), suggesting a unique role of LA in transcriptional upregulation of neutral lipid synthesis pathways. We observed by confocal microscopy that macrophages treated with LA, but not BSA or PA, exhibited elevated levels of LipidTOX, GPAT1 and DGAT1(Figure 2I). Quantitative measurements showed a significant increase of lipid accumulation (LipidTOX), GPAT1 and DGAT1 proteins in the LA-treated vs. the BSA or PA groups (Figure 2J-2M). Moreover, intracellular flow cytometric staining (Figure S2G-S2I) and quantification by mean fluorescent intensity (MFI) (Figure S2J-S2L) also confirmed our observations that unsaturated FAs significantly increased lipid accumulation by enhancing GPAT/DGAT-mediated lipid accumulation in macrophages.
C/EBPα transcriptionally controls LA-induced TAG synthesis
To determine how unsaturated LA transcriptionally upregulated gene expression in the TAG synthesis pathway, we searched for transcription factor binding sites, focusing on the pooled genes related to TAG synthesis pathways. Two transcriptional factors, C/EBPα and C/EBPβ, were predicted to commonly bind the promotor regions of these genes (Figure S3A). When macrophages were treated with PA or LA, C/EBPα (Figure 3A), but not C/EBPβ (Figure S3B), was significantly upregulated by LA treatment. Confocal microscopy showed that C/EBPα was mainly localized in the cytosol with BSA and PA treatment but was significantly upregulated in the nuclei with LA treatment (Figure 3B, 3C). In contrast, C/EBPβ was present in both cytosol and nuclei regardless of PA or LA treatment (Figure S3C, S3D). These results suggest that LA treatment induces specific activation of C/EBPα in macrophages.
To further dissect the role of C/EBPs in LA-treated macrophages, we knocked down C/EBPα (Figure 3D) and C/EBPβ (Figure S3E), respectively, in macrophages. LA-induced gene expression of Gpam1, Dgat1, Dgat2 was abrogated when C/EBPα was silenced (Figure 3E-3G). Moreover, the uptake of FA (Cd36), lysosome lipase (Lipa) and mitochondrial oxidation (Cpt1b) were significantly increased in C/EBPα-knockdown macrophages (Figure 3H-3J), suggesting that C/EBPα knockdown not only inhibited lipid synthesis, but also promoted lipolysis and oxidation. As such, C/EBPα knockdown significantly inhibited LA-induced neutral lipid accumulation as shown by the staining of BODIPY (Figure 3K) and Oil Red O (Figure 3L, 3M) in macrophages. By contract, C/EBPβ silencing did not affect the expression of Gpam1, Cd36, and Lipa expression (Figure S3F-S3H). Although C/EBPβ knockdown appeared to inhibit the expression of Dgat1 and Dgat2, it also inhibited Cpt1b-mediated FA oxidation (Figure S3I-S3K). Overall, C/EBPβ knockdown resulted in lipid accumulation in response to LA treatment in macrophages (Figure S3L). Altogether, these data suggest that C/EBPα, but not C/EBPβ, is a critical transcriptional factor controlling LA-induced TAG synthesis and LD formation in macrophages.
FABP4 mediates LA-induced C/EBPα activation
Given the insolubility of long chain FAs, it was of great interest to dissect how LA, unlike SA, specifically activated C/EBPα in macrophages. As FA chaperones, FABPs bind different FAs, facilitating their transport and responses. We measured the profile of common FABP members in macrophages and demonstrated that FA treatment mainly induced expression of FABP4, but not FABP3 and FABP5, in macrophages (Figure 4A-4C). Analyzing the TCGA human breast cancer database, we found that compared to other FABP family members FABP4 was mostly correlated with C/EPBα (Figure S4A, S4B), but not with other C/EBP members (Figure S4C). The positive associations of FABP4 with C/EBPα and key TAG synthesis genes were also evidenced in multiple GEO datasets (Figure S4D-S4F).
To gain insights into our observations, we performed single cell RNA-sequencing using splenic macrophages. UMAP plots showed that macrophages consisted of 13 clusters based on untargeted gene expression profiles (Figure S4G). FABP4 was enriched in clusters 0, 1 and 2 (Figure 4D). Compared to other non-enriched clusters (Fabp4-), GO molecular function analysis showed that FABP4 was mainly involved in protein-lipid complex, lipid binding and signaling receptors (Figure S4H). Moreover, Fabp4 enriched clusters (Fabp4+) were highly associated with genes known to be critical for lipid uptake and accumulation, including Cd36, PPARγ, CEBPA, Plin2 (Figure 4E-4I). In contrast, Fabp4 enrichment was not related to Fabp5, Acsl1, Lipa, Fasn and Cpt1b (Figure 4J, Figure S4I-S4L), suggesting a unique role of FABP4 in lipid uptake and storage in macrophages.
Confocal microscopy showed that FABP4 was mainly expressed in the cytosol in BSA- or PA-treated cells, whereas LA treatment led to FABP4 present both in the cytosol and nuclei (Figure 4K), suggesting a LA-induced FABP4 nuclear translocation effect for potential nuclear transactivation. To confirm the role of FABP4, we measured C/EBPα expression in FABP4-deficienct macrophages. Deletion of FABP4 in macrophages abrogated LA-induced C/EBPα expression in nuclei (Figure 4L, 4M). We further confirmed that FABP4 deficiency significantly blocked LA-induced expression of C/EBPα (Figure S4M, S4N), but not C/EBPβ (Figure S4O, S4P), in primary peritoneal macrophages and bone marrow-derived macrophages. Collectively, these data demonstrated a pivotal role for FABP4 in mediating LA-induced C/EBPα activation in macrophages.
FABP4 expression enhances lipid accumulation in macrophages
Given the critical role of FABP4 in activating the LA-C/EBPα axis, we reasoned that FABP4 deficiency suppressed LA-induced lipid accumulation in macrophages. To this end, we first measured if FABP4 deficiency reduced key enzymes of TAG synthesis in different macrophages. Using FABP4 wild type (WT) and knockout (KO) macrophage cell lines (Figure 5A), we demonstrated that upregulation of Gpam1, Dgat1, and Dgat2, but not Gpam4 and Agpat2, in response to LA treatment in WT macrophages, was significantly reduced when FABP4 was genetically depleted (Figure 5B-5F). Similarly, using peritoneal macrophages purified from WT and KO mice, we confirmed that FABP4 deficiency significantly reduced the expression of LA-induced Gpam1, Dgat1, Dgat2 (Figure S5A-S5D). Of note, FABP4 deficiency also reduced expression of other lipid transporters, including Cd36 and Fabp5 (Figure S5E-S5F), suggesting a general inhibition of fatty acid uptake and metabolism in the absence of FABP4 in primary macrophages.
To evaluate the role of FABP4 in lipid accumulation, we measured GPAT1 and DGAT1 protein expression and lipid droplet formation in LA-treated WT and KO macrophages. Confocal microscopy showed that GPAT1, DGAT1 and lipidTOX were colocalized in the cytoplasm of WT macrophages whereas FABP4 deficiency reduced the expression of GPAT1, DGAT1 and lipidTOX in KO macrophages (Figure 5G). Quantitative analysis demonstrated that GPAT1, DGAT1 and lipidTOX levels were significantly reduced when FABP4 was absent (Figure 5H-5J). Using transmission electron microscopy, we further confirmed that FABP4 deficiency compromised LA- and OA-induced lipid accumulation in macrophages (Figure 5L, Figure S5G). The role of FABP4 in lipid accumulation was corroborated by analysis of primary peritoneal macrophages from FABP4 WT and KO mice. As shown in Figure S5H and S5I, LA-induced neutral lipid accumulation in macrophages was significantly reduced when FABP4 was deficient. Collectively, these data suggest that FABP4 plays a pivotal role in TAG synthesis and neutral lipid accumulation in macrophages.
Macrophage expression of FABP4 promotes breast cancer migration
To further dissect the functionality of FABP4 in TAMs, we analyzed FABP4-associated gene pathways in the TCGA breast cancer datasets. Interestingly, FABP4 expression was highly associated with the lipolysis regulation pathway, including genes of β-adrenergic receptors (Adrb2), G-protein-coupled receptor signaling (Adcy4), and lipolysis enzymes (Pnpla2, Lipe) (Figure S6A-S6D). When FABP4 WT and KO macrophages were treated with PA or LA, FABP4 deficiency impaired LA-induced lipolysis in macrophages as evidenced by significantly reduced levels of Adrb2, Adcy4, Pnpla2 and Lipe (Figure 6A-6D), suggesting a critical role of FABP4 in mediating lipolysis-associated pathways in macrophages. Given the protumor role of lipid accumulation in TAMs, we treated FABP4 WT or KO macrophages with PA, LA or BSA, and evaluated their protumor function using trans-well tumor migration assays (Figure 6E). As shown in Figure 6F and 6G, compared to BSA- or PA-treated WT macrophages, LA-treated macrophages greatly promoted MDA-MB-231 cell migration. Notably, FABP4 deficiency significantly reduced LA-mediated tumor migration. Using E0771 mammary tumor cells, we confirmed the essential role of FABP4 in mediating LA-induced tumor migration effect in macrophages (Figure 6H, 6I). The FABP4/LA-induced tumor migration effect was not due to tumor proliferation as Ki67 expression was similar among different FA-treated groups (Figure S6E). Instead, lipid droplets formed by LA in WT macrophages were depleted following coculture with tumor cells (Figure 6J), showing the pro-migration effect of FABP4-mediated lipolysis. Furthermore, we collected the conditioned media collected from FA-treated macrophages and demonstrated that only media from LA-treated macrophages promoted migration of MDA-MB-231 and E0771 in a FABP4-dependent manner (Figure S6F-6I). These data suggested that intracellular FABP4 was secreted from macrophages into the extracellular environment to mediate the pro-migration effect. Indeed, LA-treated macrophages exhibited the highest levels of FABP4 compared to other groups (Figure S6J). Altogether, FABP4 expression in macrophages was essential for LA-induced pro-tumor effects.
High expression of FABP4 in TAMs is associated with breast cancer metastasis
Recently, meta-analysis demonstrated that a high density of TAMs, especially CD163+ TAMs, predicted poor survival outcomes in breast cancer4. When we analyzed pro-tumor activity of TAMs using human breast cancer tissue, we verified that high expression of the macrophage marker CD163 was significantly associated with a reduced overall survival of these breast cancer patients in multiple breast cancer datasets (Figure S7A-S7D). To verify the results from these publicly accessible datasets, we collected breast cancer tissue specimens from a cohort of 59 women with different subtypes of breast cancer (Table S1) and performed immunohistochemical (IHC) staining. Interestingly, CD163+ TAMs were positively correlated with breast tumor size (Figure 7A, 7B), corroborating the pro-tumor function of CD163+ macrophages in breast cancer. We also noticed that compared to lean patients, obese patients had more CD163+ macrophages in the TME (Figure S7E, S7F), suggesting that dysregulated lipids in obese patients promoted macrophage tumor infiltration. Given the pivotal role of FABP4 in lipid metabolism in macrophages, we analyzed FABP4 expression in CD163+ TAMs. Interestingly, FABP4 expression significantly correlated with CD163+ macrophages (p<0.001) (Figure 7C). Among the cohort, 22% of patients exhibited lymph node and distant metastasis (Figure 7D). When we compared macrophages and FABP4 expression in tumor tissues in patients with or without tumor metastasis (Figure 7E), we showed that tumors from metastatic patients had higher expression of CD163+ macrophages (Figure 7F) and FABP4 expression (Figure 7G). We also examined the association of other variables, including age, tumor grade, multifocality, lymphovascular invasion, mortality, hormone status, with CD163 and FABP4 expression in tumor tissues. Higher tumor grade and mortality were associated with more FABP4 expression in macrophages (Figure 7H, Table S2). Moreover, triple negative breast cancer exhibited higher FABP4 expression in macrophages than ER+ or HER2+ breast cancer (Figure S7G, S7H). Collectively, CD163+ TAMs, especially those with FABP4 expression, are positively associated with tumor growth and metastasis in breast cancer patients.
Discussion
Breast cancer is the most prevalent cancer in women globally 36. The World Health Organization reported in 2020 that 2.3 million women were diagnosed with breast cancer, with 685,000 deaths from the disease 36,37. Given that metastatic breast cancer is the leading cause of breast cancer-related death38, identifying new cellular and molecular mechanisms underlying breast cancer metastasis is an urgent need for breast cancer research. The TME of breast cancer is lipid enriched, and these lipids can be taken up by macrophages, leading to their alternative activation39. Alternative activated macrophages are known to stimulate angiogenesis and promote tumor progession16. However, the molecular mechanisms by which extracellular lipids contribute to intracellular lipid accumulation and pro-tumor functions in macrophages are largely unexplored.
As professional phagocytes, macrophages are engaged with uptake of extracellular lipids to mitigate their toxicity and to process them for use by themselves or adjacent cells40. Macrophages have evolved to mainly express FABP4 and FABP5 to facilitate FA transport and metabolism. We previously demonstrated that heterogenous macrophages exhibit different FABP expression profiles and activities. For instance, FABP5 expression in skin macrophages facilitates high fat diet-induced IL-1 family cytokine responses (e.g., IL-1β, IL-36γ) while FABP4 expression in TAMs promotes oncogenic IL-6/STAT3 signaling19,41,42. We further demonstrated that heterogenous macrophages exhibit a unique FABP expression profile. FABP4 is prominently expressed in CD11b+F4/80+CD11c−MHCII− macrophage subsets whereas FABP5 is mainly expressed in CD11b+F4/80+MHCII+CD11c+ macrophage subsets19,33,43, suggesting a distinct role of individual FABP family members in macrophages19,44. Interestingly, when macrophages were exposed to different species of dietary FAs, FABP4 appeared to be more responsive to FA treatment. Notably, FABP4 was mainly located in the cytoplasm under PA treatment but translocated to the nucleus in response to LA treatment. This was consistent with previous studies showing LA as an activating ligand for FABP4 nuclear translocation and PPARγ activation45. Considering that PPARγ induces CEBPα expression by binding to its enhancer and they synergistically enhance lipid accumulation in adipocytes46,47, we demonstrated that LA treatment specifically upregulated CEPBα expression and induced lipid droplet formation in macrophages. Importantly, CEBPα transcriptionally upregulated key enzymes for TAG synthesis, including GPAT1 and DGATs. Moreover, silencing CEBPα expression significantly inhibited LA-induced GPAT1 and DGAT1 expression and lipid droplet accumulation. Therefore, our data suggest a previously underappreciated axis of LA/FABP4/CEBPα in mediating lipid accumulation in macrophages.
To determine the role of the accumulated lipids in macrophages, we noticed that FABP4 expression was not only associated with the LA/CEBPα/DGAT-mediated lipid accumulation pathway, but also highly correlated with the β-AR/ATGL/HSL-mediated lipolysis pathway in breast cancer. Genetic deletion of FABP4 inhibited both lipid accumulation and lipolysis pathways, confirming a critical role of FABP4 in lipid metabolism in macrophages. When breast cancer cells were cocultured with FA-exposed macrophages, LA-treated macrophages dramatically promoted breast cancer cell migration as compared to PA- or BSA-treated cells, suggesting a specific effect of LA-mediated lipid accumulation and utilization in macrophages. Moreover, FABP4 deficiency impaired LA-mediated protumor migration effect of macrophages, further supporting that FABP4-mediated lipolysis in macrophages was critical for lipid utilization by breast cancer cells.
TAMs are the most abundant immune cells in breast cancer. To interrogate TAM’s role in the TME, M1/M2 dichotomy has been commonly proposed to polarize TAM functions. However, there have been no convincing markers or precise molecular mechanisms to determine TAM functions in vivo. By analyzing FABP4 expression in TAMs with human breast cancer specimens, we found that FABP4 expression in TAMs, especially CD163+ TAMs, correlated directly with tumor size, grade, metastasis and even patient survival. Considering the critical role of FABP4 in mediating lipid accumulation and lipolysis, it might function as a key player linking extracellular lipids to lipid storage and utilization in TAMs. Of note, compared to lipid-processing macrophages, most epithelial cell-derived cancer cells do not express FABP4, thus FABP4 may serve as a functional marker for lipophilic pro-tumor TAMs.
It is worth noting that dietary fats are linked to both breast cancer incidence and survival in epidemiologic studies48. However, the exact role of major dietary FAs, including PA, SA, OA, and LA, in breast cancer risk and progression remains unclear. Given the observations that saturated fat intake increases the risk of cardiovascular diseases, the American Dietary Guidelines recommend the consumption of unsaturated fats over saturated fats in our diets (https://www.dietaryguidelines.gov/). However, data from the present study raise the concerns about the consumption of unsaturated fats, especially LA, in patients with breast cancer. Unsaturated FAs can induce lipid accumulation in macrophages, which can subsequently be mobilized to facilitate breast cancer migration and metastasis. Furthermore, we demonstrated that FABP4 plays a critical role in facilitating unsaturated FA-induced storage and lipolysis. Upon activation of LA, FABP4 trafficks between the cytosol and nucleus to mediate lipid accumulation. In the presence of external factors (e.g., tumors), FABP4 can be secreted from macrophages during lipolysis, which in turn enhances the utilization of FA ligands by cancer cells. Thus, blocking FABP4 lipid binding and secretion might represent novel strategies for preventing lipid utilization by cancer cells.
In summary, our studies have several implications. First, as professional phagocytes, macrophages uptake different lipids. Unlike saturated FAs, unsaturated FAs are preferentially accumulated in macrophages through the FABP4/CEBPα pathway. Secondly, TAMs with lipid accumulation facilitate breast cancer progression through the FABP4-dependent lipolysis and lipid utilization pathways. Thirdly, secreted FABP4/FAs might offer a previously underappreciated mechanism for promoting breast tumor metastasis potential (Figure 7I). Thus, developing therapies that block the activity of the secreted FABP4 might provide a new avenue for the treatment of breast cancer.
Limitations of the study
In this study, we have identified FABP4 as a crucial lipid chaperone responsible for mediating lipid accumulation and lipolysis in TAMs. However, the mechanisms by which FABP4/FA complexes secreted from TAMs interact with tumor cells and mediate tumor metastasis remain unknown. While some potential FABP4 receptors, such as membrane phospholipids 49, desmoglein 2 50, cytokeratin 151, have been reported in various cell types, further investigations are needed to determine how FABP4 and its lipid ligands facilitate tumor metastasis - either by initiating oncogenic signals, by providing energy sources or both.
Acknowledgements
We thank the Iowa Institute of Human Genetics at the University of Iowa for single cell RNA-sequencing and analysis. We also thank the Holden Comprehensive Cancer Center’s P30 grant which partially funds the Breast Molecular Epidemiology Resource (BMER) (P30CA086862). B.L. thank the funding support from NIH grants U01CA272424, R01CA180986 and R01AI137324. This material should not be interpreted as representing the viewpoint of the U.S. Department of Health and Human Services, the National Institutes of Health, or the National Cancer Institute.
Declaration interests
Authors declare no competing interests.
Methods
Key resources table
Resource availability
Lead contact
Further information and requests for reagents may be directed to and will be fulfilled by the Lead Contact, Bing Li (bing-li@uiowa.edu).
Materials availability
All unique/stable reagents generated in this study are available from the lead contact with a completed materials transfer agreement.
Data and code availability
All data generated in this study will be made available upon request from the Lead Contact. This paper reports single cell sequencing data (GSE262196). Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.
Experimental model and study particpant details
Mice
Fabp4−/− mice and their wild-type (WT) mice (C57B/6 background) were maintained and bred under specific pathogen free conditions with water and regular chow ad libitum in the animal facility of the University of Iowa in accordance with the approved protocol by the University of Iowa Institutional Animal Care and Use Committee (#1042385). As breast cancer mainly occurs in female, female mice at 8-12 weeks age were used for collection of primary peritoneal macrophages, splenic macrophages and culture of bone marrow-derived macrophages.
Patient specimens
Fresh or formalin-fixed, paraffin-embedded breast cancer tissues were collected from the Breast and Molecular Epidemiology Resources (BMER) or Department of Pathology, University of Iowa. Breast sections were cut and transported to the study team without further patient identifiers. The University of Iowa Institutional Review Board approved the study protocol (#201003791, 202107133) prior to sample acquisition.
Method details
Primary Cells
WT and age matched FABP4−/− mice were euthanized by CO2 inhalation and death was confirmed via subsequent cardiac puncture. Peritoneal macrophages were isolated by injecting 10mL PBS (Gibco) into the mouse peritoneal cavity and incubated with 3 minutes with periodic abdominal palpation. EtOH was used to sterilize the surgical site and the skin of the abdomen was reflected. Using a 10mL syringe coupled with a 25G needle (Becton Dickinson and Company), PBS from the peritoneal cavity was aspirated, moved to a 15mL conical (Avantor) tube, centrifuged, and resuspended in RPMI media with 10% FBS. To obtain bone marrow derived macrophages (BMM) femurs were dissected, muscle tissue was thoroughly removed, and marrow was flushed with PBS into a 15mL conical tube by way of a 10mL syringe coupled with a 25G needle. Isolated marrow cells were resuspended in red blood cell lysis buffer (Tonbo Biosciences) for 15 minutes on ice, washed with PBS, filtered through 40µm cell strainers (VWR) and plated on 100mm culture dishes (Greiner Bio-One) at a concentration of 5×106 cells/mL in RPMI media with 10% FBS and 30ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF; BioLegend). After 48 hours and again after 120 hours from initial plating cell culture media was replaced with fresh media containing M-CSF. Cells were scrutinized after 168 hours in culture.
Cell Lines
Immortalized macrophage cell lines were established from FABP4−/− (FABP4−/− macrophages) or WT mice (WT macrophages) as described previously33,52,53. Briefly, bone marrow derived macrophages were culture as outlined above however following differentiation J2-virus conditioned media was used for 2 hours and replaced with M-CSF media. This procedure was repeated after 24 hours. Subsequently M-CSF media was removed step-wise over the course of 2 weeks at which point cells could be effectively passages. This procedure was completed for marrow cells obtained from a C57Bl/6J mouse as well as aged match FABP4−/− mouse. Breast cancer cell migration assays were completed using E0771 mouse mammary gland cells (CH3 Biosystems) and MDA-MB 231 human mammary gland cells (ATCC).
Free fatty acid (FFA) preparation
Due to their insolubility, all FFAs were conjugated with bovine serum albumin (BSA) as described previously33,44,54,55. Briefly, all FFAs used in the current study were purchased from Nu-Chek Prep (MN). PA (5 mM), SA (5 mM), OA (5 mM) or LA (5 mM) were respectively conjugated with 2 mM of endotoxin-free, FA-free BSA in PBS. FA-BSA conjugates were sonicated until dissolved. All FA-BSA conjugates or BSA control were filtered through a 0.2-μM sterile filter before use in cellular studies.
RNAi
Predesigned duplex small interfering RNA was obtained from Integrated DNA Technologies (IDT) specific to C/EBPα and C/EBPβ. J2-Immortalized macrophages were transfected using jetPRIME (Polyplus) as instructed by the manufacture. Efficacy of RNAi was confirmed by RT-PCR for each experiment.
Quantitative RT-PCR
Cells were lysed and RNA was extracted using the PureLink RNA mini kit (Invitrogen) as per manufactures instructions. RNA concentration was determined and standardized by the aid of a photospectrometer and complementary DNA was synthesized by use of QuantiTect reverse transcription kit (Qiagen). Power SYBR Green PCR Master Mix (Applied Biosystems) was included with target primers (Table S3) on MicroAmp optical 384-well reaction plates (Applied Biosystems) and a QuantStudio 7 Flex (Thermo Fisher) and the provided softeware (Design and Analysis Software V2.3, Thermo Fisher) was used to determine relative mRNA concentration by way of ΔΔCt method with hprt1 as an internal control. Standard PCR reaction parameters were used: 95°C hold stage for 10 minutes, 40 cycles of PCR reaction of 95°C for 15 seconds and 60°C for one minute, and a melt curve stage used 0.5°C steps from 60°C to 65°C.
Seahorse Cell Mito Stress
The Seahorse mitochondrial stress test (Agilent) was performed as specified by the manufacturer. Briefly, cells were plated at a concentration of 5×104 per well in RPMI media with 10% FBS. Before the assay cells were wash twice with RPMI-XF media with no FBS and incubated at 37°C for 15 minutes during equipment initialization. supplemented with 100µM BSA, PA, SA, OA, or LA. The acute injection protocol was utilized where some wells (n=5 for each FA for each genotype) received injections of RPMI-XF supplemented with 200µM BSA, PA, SA, OA, or LA, and all wells subsequently received injections of 2.5µM oligomycin, 1µM FCCP, and finally 1µM Rotenone/Antimycin/Hoechst. Assays were ran using the XFe96 extracellular flux analyzer (Agilent) coupled with automated normalization based on live cell number at the conclusion of the assay.
Breast Cancer Tissue immunohistochemistry
Formalin fixed Paraffin Embedded (FFPE) tissue specimens were deparaffinized in xylene and rehydrated through exposure to graded ethanol solutions. Antigen retrieval was performed by incubating the slides in 10 mM Citrate buffer pH6.0 at 95°C for 15 min. Then, quenching of endogenous peroxidase activity was performed by incubating the slides in BLOXALL Endogenous Blocking Solution (Vector Laboratories, SP-6000) for 10 min at room temperature. Slides were incubated with a primary antibody overnight at 4 °C, a secondary antibody for 30 min at room temperature (All the primary and secondary antibodies used in this study are summarized in key resources table). DAB peroxidase substrate (Vector Laboratories, SK-4100) or Vector Red alkaline phosphatase substrate (Vector Laboratories, SK-5100) were used for staining. Counterstain was performed by incubating the slides in Hematoxylin (Leica Gill III 3801541) for 10 seconds at room temperature. Slides were mounted with VectaMount® Express Mounting Medium (vector laboratories, H-5700-60), and were scanned by slide scanner (Leica Aperio GT 450) for quantification analysis. Hot spot quantification of CD163 and FABP4 expression in macrophages (per 1×2 mm2 area within the tumor tissue) was automatically calculated by the HALO software (Indica Labs).
Transmission Electron Microscopy
Cells were fixed with 2.5% glutaraldehyde (in 0.1 M Sodium cacodylate buffer [pH 7.4]) overnight at 4°C. samples were postfixed with 1% Osmium tetroxide for 1h and then rinsed in 0.1M Sodium cacodylate buffer. Following serial alcohol dehydration (50%, 75%, 95%, and 100%), the samples were embedded in Epon 12 (Ted Pella, Redding, CA). Ultramicrotomy was performed, and ultrathin sections (70 nm) were poststained with uranyl acetate and lead citrate. Samples were examined with a Hitachi HT-7800 transmission electron microscope (TEM) (Tokyo, Japan).
Immunogold analysis for FABP4 in macrophages
Cells were fixed with 4% paraformaldehyde (PFA), and then were dehydrated in graded concentrations of ethanol and embedded in LR White resin (Electron Microscopy Sciences, 14380). Ultrathin sections (70 nm) on grids were incubated with blocking solution (Aurion, 905.003) for 1 hr, and then incubated with primary antibody overnight at 4 °C, and then incubated with secondary antibody (Aurion, Rabbit-anti-Goat IgG (H&L) Ultra Small) for 2 hrs. Grids were re-fixed with 1% glutaraldehyde for 15 min and were incubated in sliver enhancement (Aurion, 500.033) for 25 min. Sections were then stained with uranyl acetate and lead citrate and examined with a Hitachi HT-7800 transmission electron microscope (TEM) (Tokyo, Japan).
Oil Red O staining
For each use Oil Red O powder (Alfa Aesar) was solubilized in 100% isopropanol (75mg in 25mL) and mixed on a orbital shaker for 30 minutes to make a stock solution. For a working solution Oil Red O stock solution was used at a ratio of 3 parts Oil Red O to 2 parts ddH2O and mixed on an orbital shaker for 10 minutes. The working solution was filtered twice through 0.2 µm syringe filter and used immediately on 4% PFA fixed cells. During the second of 3 washes with ddH2O, DAPI was included at 1:5000 for nuclear identification. Images were taken with an ECHO Revolution2 (ECHO) epifluorescence microscope and processed using ImageJ/Fiji. Following image collection, for the purpose of semi-quantitative analysis, samples were washed with 60% isopropanol 3 times for 5 minute each and the residual Oil Red O was solubilized with 100% Isopropanol and absorbance was assessed by spectrophotometer at 492nm, 100% isopropanol was used as a background.
Flow Cytometry
The following anti-mouse conjugated antibodies were used to label macrophages surface receptors from peritoneal and bone marrow derived cells: PE/Cyanine7-F4/80, BV711-CD11c, BV605-I-A/I-E (BioLegend), BUV737-CD11b, and BUV563-CD45 (BD Biosciences). Unconjugated antibodies were used for quantification of intracellular markers: m/rFABP4 (R&D Systems), GPAM (Invitrogen), DGAT1 (Sigma-Aldrich), C/EBPα (Cell Signaling), C/EBPβ (DSHB). The following organelle dyes were used: BODIPY 493/503 (Lipid Droplets; Molecular Probes), Cell Navigator Live Cell Endoplasmic Reticulum Staining Kit Red (ATT Bioquest), and LysoTracker Blue DND-22 (Invitrogen). Dead cells were identified and removed from analysis via Ghost Dye Violet 510 (Tonbo Biosciences). Briefly, live cells were scrutinized unless fixation was required for intracellular targets in which case 4% PFA was used for 30 minutes to fix the cells and 0.2% Triton X-100 in PBS was used for permeabilization. Antibodies or organelle dyes were incubated with cells for 30 minutes on ice in the dark. When necessary secondary antibodies were incubated with cells for 1 hour on ice in the dark. Data was acquired using a Cytek Aurora 5-laser flow cytometer or ImageStream cytometer (CytekBio) and subsequently analyzed FlowJo V10 (BD Biosciences).
Migration Assay
For the migration assay, we treated WT and FABP4 KO macrophages with BSA, PA, or LA (100μM) for 4 hours in a 24-well plate. After washing them three times with PBS, we seeded E0771 or MDA-MB 231 cells (5 × 104) into transwell inserts with a pore size of 8 μm (Corning). These cells were incubated with the FA-treated macrophages, respectively, to allow tumor cells to migrate. After a 24-hour culture at 37 °C, we removed the tumor cells inside the transwells that had not migrated using cotton swabs. The tumor cells that had migrated to the outside of the insert membrane were fixed with 4% PFA for 20 minutes and then stained with 0.1% crystal violet for 10 minutes. We counted the numbers of migrated E0771 and MDA-MB 231 cells in 12 random high fields under a microscope. Each assay was performed in triplicate wells. Additionally, in some experiments, we used conditional media (RPMI-1640 without FBS) collected from BSA or FA-treated WT and KO macrophages to coculture with tumor cells and observe medium-induced tumor cell migration.
Single cell RNA-seq assay
Macrophages from mouse spleen were used for the 10 x Genomics 3’ expression single cell assay. Briefly, splenocytes were isolated from two pairs of age-matched WT and FABP4−/− female mice. Dead cells were excluded using Zombie-Violet and anti-CD16/32 antibody was used to block Fc receptors before additional staining with Alexa Fluor 488-conjugated anti-mouse F4/480 antibody and APC-conjugated anti-mouse CD11b antibody. The F4/80+/CD11b+/Zombie-Voilet-cells were sorted using a FACS Aira III instrument and resuspended at a concentration of 1000 cell/μL cold PBS with 0.04% non-acetylated BSA. An equal number of 5000 targeted cells from each sample were prepared to create GEMs. The GEM generation/barcoding, post GEM-reverse transcription cDNA amplification, and library construction were performed according to the manufacturer’s guidelines.
Single cell data analysis
Single cell gene counting was performed by Cell Ranger (10X Genomics, version 7.1.0) using the refdata-gex-mm10-2020-A reference transcriptome. The resulting data matrices were subsequently imported into R (version 4.2.3) and analyzed using the Seurat package (version 4.9.9). Cells with less than 200 features or with percent mitochondrial gene expression greater than 5% were excluded from the analysis. The data across samples was integrated using the IntegrateData function. Gene expression was normalized and scaled using the default parameters. Based on visual inspection of the elbow plot, the first 30 Principal Components (PC) were used in UMAP-based dimensional reduction. The FindClusters function, with a resolution of 0.5, was then used to assign cells to clusters. The FindMarkers function was used to identify genes differentially expressed between wild-type and knockout samples or between different clusters within the same sample type. Feature plots and violin plots were used to visualize the results.
ELISA for FABP4 analysis
FABP4 excreted from cultured cells were analyzed with CircuLex Mouse FABP4 ELISA Kit (Medical and Biological Laboratories) as instructed by the manufactures. Briefly, for the ELISA 100uL of conditioned media was incubated in a 96-well plate precoated with FABP4 antibody, washed four times, incubated with the supplied secondary antibody, washed, and incubated with the developer to finally analyze with a spectrophotometer using a ratio of the absorbance at 450nm and 550nm.
Quantification and statistical analysis
All data were presented as the mean ± SD unless notified specifically. All experiment as indicated were performed by at least three independent experiments or technical replicates. For in vitro experiments, a two-tailed, unpaired student t-test, two-way ANOVA followed by Bonferroni’s multiple comparison test, were performed by GraphPad Prism 9. For in vivo experiments analyzing associations of FABP4, TAMs and other factors in breast cancer patients, the Kruskal-Wallis test was applied to compare whether the outcome variable is significantly different across the different levels of a categorical predictor variable. Multiple linear regression models were employed to examine the association between the predictor variables and the outcome, where log transformation and Box Cox transformation were used to meet the underlying model assumption for normality. All statistical analyses were carried out in the statistical analysis software R. A statistics test is claimed to be significant if the p-value is less than 0.05.
References
- 1.Regulation of macrophage functions by FABP-mediated inflammatory and metabolic pathwaysBiochim Biophys Acta Mol Cell Biol Lipids 1866https://doi.org/10.1016/j.bbalip.2021.158964
- 2.Tumour-educated macrophages promote tumour progression and metastasisNat Rev Cancer 4:71–78https://doi.org/10.1038/nrc1256
- 3.Macrophages: obligate partners for tumor cell migration, invasion, and metastasisCell 124:263–266https://doi.org/10.1016/j.cell.2006.01.007
- 4.Breast Cancer Survival Outcomes and Tumor-Associated Macrophage Markers: A Systematic Review and Meta-AnalysisOncol Ther 11:27–48https://doi.org/10.1007/s40487-022-00214-3
- 5.Stromal gene expression predicts clinical outcome in breast cancerNat Med 14:518–527https://doi.org/10.1038/nm1764
- 6.The macrophage colony-stimulating factor 1 response signature in breast carcinomaClin Cancer Res 15:778–787https://doi.org/10.1158/1078-0432.CCR-08-1283
- 7.Macrophage Biology and Mechanisms of Immune Suppression in Breast CancerFront Immunol 12https://doi.org/10.3389/fimmu.2021.643771
- 8.Targeting tumor-associated macrophages as a novel strategy against breast cancerJ Clin Invest 116:2132–2141https://doi.org/10.1172/JCI27648
- 9.Shigella mediated depletion of macrophages in a murine breast cancer model is associated with tumor regressionPLoS One 5https://doi.org/10.1371/journal.pone.0009572
- 10.Macrophages orchestrate breast cancer early dissemination and metastasisNat Commun 9https://doi.org/10.1038/s41467-017-02481-5
- 11.Monocyte and macrophage heterogeneityNat Rev Immunol 5:953–964https://doi.org/10.1038/nri1733
- 12.Functional plasticity of macrophages: reversible adaptation to changing microenvironmentsJ Leukoc Biol 76:509–513https://doi.org/10.1189/jlb.0504272
- 13.Transcriptome-based network analysis reveals a spectrum model of human macrophage activationImmunity 40:274–288https://doi.org/10.1016/j.immuni.2014.01.006
- 14.Alternative activation of macrophages: an immunologic functional perspectiveAnnu Rev Immunol 27:451–483https://doi.org/10.1146/annurev.immunol.021908.132532
- 15.Exploring the full spectrum of macrophage activationNat Rev Immunol 8:958–969https://doi.org/10.1038/nri2448
- 16.Macrophages, innate immunity and cancer: balance, tolerance, and diversityCurr Opin Immunol 22:231–237https://doi.org/10.1016/j.coi.2010.01.009
- 17.Tumor-associated macrophages: a molecular perspectiveInt Immunopharmacol 2:1045–1054https://doi.org/10.1016/s1567-5769(02)00064-4
- 18.Macrophage activation and polarization: nomenclature and experimental guidelinesImmunity 41:14–20https://doi.org/10.1016/j.immuni.2014.06.008
- 19.Expression of Adipocyte/Macrophage Fatty Acid-Binding Protein in Tumor-Associated Macrophages Promotes Breast Cancer ProgressionCancer Res 78:2343–2355https://doi.org/10.1158/0008-5472.CAN-17-2465
- 20.Itaconic acid mediates crosstalk between macrophage metabolism and peritoneal tumorsJ Clin Invest 128:3794–3805https://doi.org/10.1172/JCI99169
- 21.CD4(+) T cells regulate pulmonary metastasis of mammary carcinomas by enhancing protumor properties of macrophagesCancer Cell 16:91–102https://doi.org/10.1016/j.ccr.2009.06.018
- 22.HRG inhibits tumor growth and metastasis by inducing macrophage polarization and vessel normalization through downregulation of PlGFCancer Cell 19:31–44https://doi.org/10.1016/j.ccr.2010.11.009
- 23.Anti-inflammatory effect of IL-10 mediated by metabolic reprogramming of macrophagesScience 356:513–519https://doi.org/10.1126/science.aal3535
- 24.Immunometabolism governs dendritic cell and macrophage functionJ Exp Med 213:15–23https://doi.org/10.1084/jem.20151570
- 25.Characterizing Macrophage Diversity in Metastasis-Bearing Lungs Reveals a Lipid-Associated Macrophage SubsetCancer Res 81:5284–5295https://doi.org/10.1158/0008-5472.CAN-21-0101
- 26.Lipid-loaded tumor-associated macrophages sustain tumor growth and invasiveness in prostate cancerJ Exp Med 219https://doi.org/10.1084/jem.20210564
- 27.Lipid accumulation in macrophages confers protumorigenic polarization and immunity in gastric cancerCancer Sci 111:4000–4011https://doi.org/10.1111/cas.14616
- 28.Lipid droplet-dependent fatty acid metabolism controls the immune suppressive phenotype of tumor-associated macrophagesEMBO Mol Med 11https://doi.org/10.15252/emmm.201910698
- 29.Dynamics and functions of lipid dropletsNat Rev Mol Cell Biol 20:137–155https://doi.org/10.1038/s41580-018-0085-z
- 30.The surface of lipid droplets is a phospholipid monolayer with a unique Fatty Acid compositionJ Biol Chem 277:44507–44512https://doi.org/10.1074/jbc.M207712200
- 31.Fatty acid-binding proteins: role in metabolic diseases and potential as drug targetsNat Rev Drug Discov 7:489–503https://doi.org/10.1038/nrd2589
- 32.SnapShot: FABP FunctionsCell 182:1066–1066https://doi.org/10.1016/j.cell.2020.07.027
- 33.Adipose Fatty Acid Binding Protein Promotes Saturated Fatty Acid-Induced Macrophage Cell Death through Enhancing Ceramide ProductionJ Immunol 198:798–807https://doi.org/10.4049/jimmunol.1601403
- 34.Uptake of oxidized lipids by the scavenger receptor CD36 promotes lipid peroxidation and dysfunction in CD8(+) T cells in tumorsImmunity 54:1561–1577https://doi.org/10.1016/j.immuni.2021.05.003
- 35.Enhancing CD8(+) T Cell Fatty Acid Catabolism within a Metabolically Challenging Tumor Microenvironment Increases the Efficacy of Melanoma ImmunotherapyCancer Cell 32:377–391https://doi.org/10.1016/j.ccell.2017.08.004
- 36.Current and future burden of breast cancer: Global statistics for 2020 and 2040Breast 66:15–23https://doi.org/10.1016/j.breast.2022.08.010
- 37.Breast and cervical cancer in 187 countries between 1980 and 2010: a systematic analysisLancet 378:1461–1484https://doi.org/10.1016/S0140-6736(11)61351-2
- 38.Tumor metastasis: molecular insights and evolving paradigmsCell 147:275–292https://doi.org/10.1016/j.cell.2011.09.024
- 39.Cell-intrinsic lysosomal lipolysis is essential for alternative activation of macrophagesNat Immunol 15:846–855https://doi.org/10.1038/ni.2956
- 40.New Insights on the Role of Lipid Metabolism in the Metabolic Reprogramming of MacrophagesFront Immunol 10https://doi.org/10.3389/fimmu.2019.02993
- 41.Consumption of fish oil high-fat diet induces murine hair loss via epidermal fatty acid binding protein in skin macrophagesCell Rep 41https://doi.org/10.1016/j.celrep.2022.111804
- 42.Epidermal Fatty Acid binding protein promotes skin inflammation induced by high-fat dietImmunity 42:953–964https://doi.org/10.1016/j.immuni.2015.04.016
- 43.Stearic Acid Induces CD11c Expression in Proinflammatory Macrophages via Epidermal Fatty Acid Binding ProteinJ Immunol 200:3407–3419https://doi.org/10.4049/jimmunol.1701416
- 44.Fatty acid-binding protein E-FABP restricts tumor growth by promoting IFN-beta responses in tumor-associated macrophagesCancer Res 74:2986–2998https://doi.org/10.1158/0008-5472.CAN-13-2689
- 45.Structural basis for activation of fatty acid-binding protein 4J Mol Biol 372:1246–1260https://doi.org/10.1016/j.jmb.2007.07.040
- 46.PPARgamma and C/EBPalpha response to acute cold stress in brown adipose tissueiScience 26https://doi.org/10.1016/j.isci.2022.105848
- 47.Peroxisome proliferator-activated receptor gamma and C/EBPalpha synergistically activate key metabolic adipocyte genes by assisted loadingMol Cell Biol 34:939–954https://doi.org/10.1128/MCB.01344-13
- 48.The Impact of Dietary Fat on Breast Cancer Incidence and Survival: A Systematic ReviewCureus 14https://doi.org/10.7759/cureus.30003
- 49.Circulating Adipose Fatty Acid Binding Protein Is a New Link Underlying Obesity-Associated Breast/Mammary Tumor DevelopmentCell Metab 28:689–705https://doi.org/10.1016/j.cmet.2018.07.006
- 50.Desmoglein 2 Functions as a Receptor for Fatty Acid Binding Protein 4 in Breast Cancer Epithelial CellsMol Cancer Res 21:836–848https://doi.org/10.1158/1541-7786.MCR-22-0763
- 51.New insights into circulating FABP4: Interaction with cytokeratin 1 on endothelial cell membranesBiochim Biophys Acta 1853:2966–2974https://doi.org/10.1016/j.bbamcr.2015.09.002
- 52.The fatty acid-binding protein, aP2, coordinates macrophage cholesterol trafficking and inflammatory activity. Macrophage expression of aP2 impacts peroxisome proliferator-activated receptor gamma and IkappaB kinase activitiesJ Biol Chem 280:12888–12895https://doi.org/10.1074/jbc.M413788200
- 53.LPS stimulation of TNF-receptor deficient macrophages: a differential role for TNF-alpha autocrine signaling in the induction of cytokine and nitric oxide productionImmunobiology 202:477–492https://doi.org/10.1016/s0171-2985(00)80105-9
- 54.Dietary Fats High in Linoleic Acids Impair Antitumor T-cell Responses by Inducing E-FABP-Mediated Mitochondrial DysfunctionCancer Res 81:5296–5310https://doi.org/10.1158/0008-5472.CAN-21-0757
- 55.Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell DeathJ Vis Exp https://doi.org/10.3791/56535
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