Periosteal SSCs have a metabolic profile conferring a resistance to stress
A. Gene set enrichment analysis (GSEA) plots comparing P-SSCs versus BM-MSCs at steady state (n=3 per group). B. Quantitative RT-PCR analysis of mRNA expression of Cdkn1a, Cdkn1c, Cdk4 relative to Actb in sorted CD45-Ter119-CD31-CD51+CD200+ BM-MSCs and P-SSCs (n=3-6 per group). C. Flow cytometric analysis of glucose uptake at steady state in CD45-Ter119-CD31-CD51+CD200+ BM-MSCs and P-SSCs (n=5 per group). D. Quantification of cellular ROS at steady state in CD45-Ter119-CD31-CD51+CD200+ BM-MSCs and P-SSCs (n=8 per group). E. Quantitative RT-PCR analysis of mRNA expression of Sod1, Gls and Gpx1 relative to Actb in sorted CD45-Ter119-CD31-CD51+CD200+ BM-MSCs and P-SSCs (n=3-7 per group). F. Schematic illustration of the protocol for the in vitro apoptosis assay. BM-MSCs and P-SSCs were isolated and digested before plating in a 10cm dish. At near confluence, cells underwent CD45 lineage depletion and plated into multi-well plates. At near confluence, medium was switched from 20% FBS to 0% FBS. Cells were analyzed at the time of medium switch and 12 hours. G. Percentage of apoptotic BM-MSCs and P-SSCs cultured under 5% O2 at baseline and 12 hours after being in 0% FBS serum conditions (n=11-12 per group). Two-way ANOVA with Tukey’s multiple comparisons test was used to determine statistical significance. Data are represented as the mean ± SEM. Unless otherwise noted, statistical significance was determined using unpaired two-tailed Student’s t test. *p<0.05. ** p<0.01. *** p<0.001. ****p<0.0001. This figure was created using BioRender.com.