Expression of immune signals in macrophage RAW264.7 and colon epithelial cells Caco-2 upon being transfected by total RNA extracted from Salmonella strains carrying different constructs.
RNA was transfected into the cell using Lipofectamine 2000 transfection reagent. (a) Expression of (a)TNF-α, (b)IL-1β, (c)MDA-5, (d)IFN-β in RAW264.7 cell line; Expression of (e)IFN-β, (f)MDA-5, (g)RIG-1 in Caco-2 cell line. The cells of no-bacteria control were treated with Lipofectamine 2000 transfection reagent without RNA.