Effect of glycolytic activators on β-cell oscillations.
(A) Schematic of glycolysis showing the targets of glucokinase activator (GKa) and pyruvate kinase activator (PKa). (B) Illustration indicating the oscillation period, active phase duration, silent phase duration, and duty cycle (active phase/period) calculated at half-maximal Ca2+. (C-E) Sample traces and comparison of period, active phase duration, silent phase duration and duty cycle before and after vehicle (0.1% DMSO) (n=11284 cells, 13 islets, 7 mice) (C), GKa (50 nM RO-28-1675) (n=6871 cells, 8 islets, 7 mice) (D) and PKa (n=10700 cells, 13 islets, 7 mice) (10 µM TEPP-46) (E). Data are displayed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by Student’s t-test.