Analysis of DUP-RAI14 exon 11 (ex 11) splicing reporter. A. Schematic of the beta globin pDUP-RAI14 ex 11 splicing reporters indicating the region of intron 10, exon 11, and intron 11 included; the inset below show the different constructs (wild type or mutant) tested. B. RT-PCR and BioAnalyzer gel-like image from RNA extracted from C2C12 cells transfected with RAI14 ex 11 wild type or deletion mutant reporters; the box plots below indicate the percent observed level of each splice variant shown (from top to bottom: unspliced RNA, *an unidentified/spurious product, the exon-included form, or exon-skipped form) with *P < 0.05, **P < 0.01, ***P < 0.001, or ****P < 0.0001. C. RT-qPCR measuring total reporter RNA level, normalized to Eef1a1, from RNA described in B., and shown as fold-change relative to WT (**P < 0.01 or ****P < 0.001 by Student’s t-test). D. RT-PCR and BioAnalyzer gel-like image from RNA extracted from C2C12 cells transfected with RAI14 ex 11 wild type or substitution mutant reporters, analyzed as described in B (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). E. RT-qPCR measuring total reporter RNA level, normalized to Eef1a1, from RNA described in D., and shown as fold-change relative to WT (*P < 0.05 or ****P < 0.001 by Student’s t-test). F. RT-PCR and BioAnalyzer gel-like image from RNA extracted from C2C12 cells transfected with RAI14 ex 11 wild type or half-site mutant reporters, analyzed as described in B (***P < 0.001, ****P < 0.0001). G. RT-qPCR measuring total reporter RNA level, normalized to Eef1a1, from RNA described in F., and shown as fold-change relative to WT (**P < 0.01 or ****P < 0.001 by Student’s t-test). Each experiment was conducted in biological triplicate.