Summary Statistics of Continuous Variables

Summary Distribution of Clinical and Pathological Characteristics

Combined Receptor Subtypes

Mutation profile of ZNF217, ZNF703, and ZNF750 genes in 23 patients.

(A) Mutation burden by gene showing the number of single nucleotide polymorphisms (SNPs) and indels, (B) Coding consequence composition illustrating the distribution of synonymous, missense, and frameshift variants, (C) Number of patients with detectable mutations per gene.

Nucleotide substitution spectrum across ZNF217, ZNF703, and ZNF750 genes in 23 patients.

Heatmap showing the distribution and frequency of nucleotide substitution classes (A/C, A/G, A/T, C/A, C/G, C/T, G/A, G/C, G/T, T/A, T/C, T/G) across the three genes, with color intensity representing mutation counts.

Gene Level Mutation Summary

Recurrent Coding Variants

Distribution of coding mutations across protein domains in ZNF217, ZNF703, and ZNF750 genes.

Lollipop plots showing the positions of coding mutations along the protein sequences of each gene, with annotated functional domains indicated.

Functional characterization of mutations across ZNF217, ZNF703, and ZNF750 genes.

(A) Mutation enrichment in protein domains showing the number of mutations occurring within and outside annotated functional domains for each gene, (B) Relative composition of mutation types illustrating the proportion of synonymous, missense, and frameshift variants across the three genes.

Recurrent mutations across ZNF217, ZNF703, and ZNF750 genes in 23 patients.

Bar plot showing the most frequently observed amino acid substitutions across the three genes, with bar length representing the number of samples harboring each mutation and colors indicating gene origin.

Combined Mutation Comparison

ZNF Expression Descriptive Statistics

ZNF Expression Descriptive Statistics

Differential expression patterns of ZNF217, ZNF703, and ZNF750 in paired breast tumor and normal tissues.

(A) Violin plots showing the distribution of normalized gene expression levels in tumor and matched normal samples for each gene. (B) Paired tumor–normal expression profiles for individual patients. (C) Histograms depicting the distribution of normalized expression values in tumor and normal tissues. (D) Boxplots of paired expression differences (tumor minus normal) for each gene.

Patient-level expression differences and relative expression patterns of ZNF217, ZNF703, and ZNF750.

(A) Ranked tumor–normal expression differences for each gene across individual patients. (B) Heatmap of z-score–normalized expression values for ZNF217, ZNF703, and ZNF750 across all samples, with color scale indicating relative expression levels.

Principal component analysis of gene expression profiles.

Scatter plot of the first two principal components based on normalized expression values of ZNF217, ZNF703, and ZNF750, with samples colored by condition (tumor and normal). Axes indicate the proportion of variance explained by each component.

Associations between gene expression and clinical variables.

(A) Scatter plots showing the relationship between body mass index (BMI) and tumor expression of ZNF217, ZNF703, and ZNF750. (B) Scatter plots showing the relationship between age and tumor expression for each gene. (C) Boxplots of tumor expression across tumor grades. (D) Boxplots of tumor expression across cancer stages.

Gene expression across receptor status categories.

(A) Boxplots of tumor expression of ZNF217, ZNF703, and ZNF750 stratified by HER2 status. (B) Boxplots of tumor expression stratified by estrogen receptor (ER) status. (C) Boxplots of tumor expression stratified by progesterone receptor (PR) status.

Multiple Regression Summary

Clinical association heatmap of ZNF217, ZNF703, and ZNF750 expression.

Heatmap showing correlation coefficients between tumor expression of ZNF217, ZNF703, and ZNF750 and clinical variables, including age, body mass index (BMI), tumor grade, cancer stage, HER2 status, estrogen receptor (ER) status, and progesterone receptor (PR) status. Color scale represents the strength and direction of correlations.