Serum susceptibility is multifactorial and varies widely across closely related S. aureus isolates. (a & b) The susceptibility of 300 bacteraemia isolates from clonal complexes CC22 and CC30 were exposed to human serum and their ability to survive this exposure quantified. The survival of each isolate was quantified in triplicate and dot represents the mean of these data. (c & d) Manhattan plots representing the statistical associations (on the y axis) between individual SNPs across the genome (on the x axis) and serum survival. The dotted red line represents the uncorrected significance threshold, and the dotted blue line represents the Sidak corrected (for multiple comparisons) threshold.

Loci associated with serum survival in the CC22 (Table 1a) and CC30 (Table 1b) collections. The SNP position is relative to the origin of replication in the reference genomes: HO 5096 0412 (CC22 and MRSA252 (CC30). Locus tags and where available gene names or putative protein functions have been provided. NTML refers to the mutant available in the Nebraska transposon library.

Functional verification of loci involved in serum sensitivity of S. aureus. The effect of the inactivation of the genes associated serum sensitivity was examined using transposon mutants of strain JE2. Of the 32 mutants tested seven were significantly affected: (a) tcaA, (b) tarK, (c) gntK, (d) ilvC, (e) arsB, (f) yhfO and (g) pdhD. The effect of the mutations on serum sensitivity was complemented by expressing the gene from the expression plasmid pRMC2 (e.g. the tcaA complementing plasmid is called ptcaA). The dots represent individual data points, the bars the mean value, and the error bars the standard deviation. Significance was determined as * <0.05, ** <0.01, *** <0.001.

TcaA mutations affect resistance to both teicoplanin and serum across multiple S. aureus backgrounds.

(a) The individual clinical isolates with polymorphism in the tcaA gene are indicated (in red) on a graph displaying the range of serum survival of the collection. (b) The structure of the TcaA protein as predicted by AlphaFold. (c) The most common tcaA SNP decreases the sensitivity of S. aureus to serum. The dots represent individual data points, the bars the mean value, and the error bars the standard deviation.

TcaA production confers increased sensitivity to several antibacterial components of serum. (a) The inactivation of the tcaA gene decreases the sensitivity of three S. aureus strains (i.e., JE2, Newman and SH1000) to serum. (b) TcaA production by the wild type and ptcaA complemented strain confers increased sensitivity to arachidonic acid (100μM), (c) TcaA production by the JE2 wild type and ptcaA complemented strain confers increased sensitivity to LL37 (5μg/ml) and (d) TcaA production by the wild type and ptcaA complemented strain confers increased sensitivity to HNP-1 (5μg/ml). The dots represent individual data points, the bars the mean value, and the error bars the standard deviation. Significance was determined as * <0.05, ** <0.01, *** <0.001.

Wall teichoic acids are released from the cell wall in the tcaA mutant to affect resistance to HDFAs and AMPs. (a) WTA was extracted from both cells and supernatant of the wild type and tcaA mutant of S. aureus and visualised on and SDS-PAGE gel stained with 1 mg/ml Alcian blue. The tcaA mutant had significantly less WTA in the cell wall but more in the supernatant. (b) The phosphate content of the cell wall WTA extracts was quantified, which verified that the tcaA mutant has significantly less WTA. (c) The inactivation of the lcpA gene increases the sensitivity of S. aureus to killing by serum. (d) The wild type strain JE2 was grown in broth supplemented with supernatant of either itself (JE2), with that from a tcaA mutant, or with that from a lcpA mutant. These supernatants had no effect on the growth of JE2 in the absence of arachidonic acid. In the presence of arachidonic acid JE2 was unable to grow when supplemented with its own supernatant, however, when supplemented with the supernatant of the two mutants, which both contain soluble WTA, JE2 was able to grow. The addition of WTA extract from another S. aureus strain (LAC) also neutralised arachidonic acid, however an equivalent extract from an isogenic WTA mutant (LAC ΔtarO) did not. (e) The charge across the cell wall of the wild type and tcaA mutant was compared using cytochrome C, where the mutant was found to be less negatively charged. The dots represent individual data points, the bars the mean value, and the error bars the standard deviation. Significance was determined as * <0.05, ** <0.01, *** <0.001, **** <0.0001.

TcaA confers increased sensitivity to teicoplanin. (a) The inactivation of the tcaA gene decreases the sensitivity of three distinct S. aureus strains (i.e., JE2, Newman and SH1000) to teicoplanin (0.5μg/ml). (b) The clinical isolates with polymorphisms in the tcaA gene were on average less sensitive to teicoplanin than those with the wild type gene. (c) The most common tcaA SNP decreases the sensitivity of S. aureus to teicoplanin. (d) The expression of the tcaA gene was quantified qRT-PCR in both subinhibitory and inhibitory concentrations of serum and teicoplanin. There was a dose dependent effect on tcaA induction for all concentrations used. (e) Exposure of JE2 to inhibitory concentrations of human serum resulted in an increase in WTA abundance in the bacterial cell wall, an effect not seen when the tcaA mutant was exposed to serum. (f) Growth of JE2 in the presence of sub-inhibitory concentrations of teicoplanin (0.5 μg/ml) over a 24h period was quantified following pre-exposure to subinhibitory concentrations of human serum (2.5%), where preexposure to serum increased sensitivity to teicoplanin. The dots represent individual data points, the bars the mean value, and the error bars the standard deviation. Significance was determined as * <0.05, ** <0.01, *** <0.001, **** <0.0001.

TcaA alters the structure of the S. aureus cell wall. (a) The rate of lysis in the presence of lysostaphin was assayed, where the strain producing was lysed at a slower rate than the tcaA mutant. A pbp4 mutant was included as a control. (b) The rate of autolysis of the bacteria in the presence of Triton x100 was assayed where the TcaA producing strain lysed at a slower rate relative to the tcaA mutant. An autolysin (atl) mutant was included as a control. (c) Transmission electron micrograph (TEM) of a wild type JE2 and tcaA mutant cell at two magnifications showing the smooth and consistent density of the cell wall when TcaA is produced, compared to the rough and patchy density of the mutant cell wall.

TcaA contributes to increased disease severity in mice and humans.

(a & b) A graph and contingency table showing data from 113 patient with S. aureus bacteraemia. The 30-day mortality rate was significantly higher for those infected with a S. aureus strain with a wild type tcaA gene compared to those with a mutated tcaA gene. (c) Mice were injected intravenously with 100 μl of PBS containing an equal mixture of wild type and tcaA mutant (2 x 107 CFUs of each). After 48 hrs, the ratio of the wild type and mutant were quantified and the relative virulence ratio calculated. In the blood and all organs tested the wild type bacteria had a competitive advantage over the mutant demonstrating its increased relative virulence. Mean±s.d. The dots represent individual data points (n=5 per group), the bars the mean value, and the error bars the standard deviation. Significance was determined as * <0.05, ** <0.01, and *** <0.001.

Strains used in this study.

PCR primers used in this study.