Identification of unique essential bacterial genes is important for not only the understanding of their cell biology but also the development of new antimicrobials. Here we report a previously unrecognized core component of the Acinetobacter baumannii divisome. Our results reveal that the protein, termed Aeg1 interacts with multiple cell division proteins, including FtsN, which is required for components of the divisome to localize to the midcell. We demonstrate that the FtsAE202K and FtsBE65A mutants effectively bypassed the need of Aeg1 by A. baumannii, as did the activation variants FtsWM254I and FtsWS274G. Our results suggest that Aeg1 is a cell division protein that arrives at the division site to initiate cell division by recruiting FtsN, which activates FtsQLB and FtsA to induce the septal peptidoglycan synthase FtsWI. The discovery of the new essential cell division protein has provided a new target for the development of antibacterial agents.
This study is useful in identifying several gain-of-function division variants that can suppress the elongated cell division defect phenotype caused by the depletion of Age1 (or AdvA as named in other studies), a known essential cell division protein in Acinetobacter baumannii. However, characterizations of AdvA's localization patterns and its interactions with other divisome proteins are incomplete due to the lack of (1) functional characterizations of fluorescent fusion proteins, (2) considerations for membrane protein topology in the bacterial two hybrid assay, and (3) lack of high-quality fluorescence images for the co-localization studies. The results do not yet support the major claim that Age1 plays a critical role in the assembly of the A. baumannii divisome.
Acinetobacter baumannii is a Gram-negative bacterial pathogen that causes a wide variety of nosocomial infections (1, 2), including urinary tract infections, gastrointestinal and skin/wound infections and secondary meningitis (3–6). The challenge posed by this pathogen is compounded by the emergence of isolates that are resistant to almost all of the available antibiotics, including some of the last resort for treatment of infections caused by bacterial pathogens, including colistin, tigecycline, and carbapenems (2, 7). As a result, the World Health Organization (WHO) has classified A. baumannii as a pathogen for which new antibiotics are urgently needed (8). Meeting this challenge requires the discovery of antimicrobials of new mechanisms with action or other mitigation strategies that enable reduction of disease burden caused by this pathogen.
Historically, the discovery of antibiotics was largely based on the search for secondary metabolites produced by soil-dwelling microbes (9, 10). The majority of clinically used antibiotic classes originate from soil bacteria, including lactams, aminoglycosides, polymyxins, and glycopeptides (11). Unfortunately, natural products discovery is now plagued by problems of so-called ‘dereplication problem’, wherein rediscovery of molecules or their close relatives (12). As a result, there has not been a novel antibiotic discovered in over 30 years using traditional methods of screening environmental samples (13). The overuse of these merely variants of existing compounds has further accelerated the problem associated with antibiotic resistance. Thus, identification of novel therapeutic targets in the pathogen may serve as an effective alternative to the problem.
A better understanding of gene function associated with A. baumannii pathogenesis and biology will provide us strategies to develop antibiotics with potentially novel mechanisms of action. One extensively explored avenue in this regard is the identification of previously unrecognized essential genes by methods enabled by new DNA sequencing technologies, including insertion sequencing (INSeq) (14), transposon sequencing (Tn-seq) (15), transposon-directed insertion site sequencing (TraDIS) (16), and high-throughput insertion tracking sequencing (HITS) (17). Application of these methods has identified genes believed to be required for bacterial viability under various conditions in such important pathogens as Streptococcus pneumoniae (15), Pseudomonas aeruginosa (18), Vibrio cholerae (19) and A. baumannii (20–22).
Similar to candidate genes identified by other methods, putative essential genes obtained by In-seq were deduced from sequencing results of surviving mutants, some of which can be false positives or negatives due to unsaturated mutant libraries or polar effects on downstream genes of the insertion sites. Using In-seq Wang et al identified 453 candidate essential genes for the growth of A. baumannii strain ATCC 17978 on rich medium (22). Among these, genes annotated as hypotheticals with orthologs in other taxonomically distant microorganisms are of particular interest because their potential role in cellular processes that can be explored as targets for the development of novel antimicrobials. We thus set out to test a subgroup of the predicted essential genes by a conditional gene deletion strategy based on the arabinose inducible promoter (23). From ten genes examined, three were found to be essential for A. baumannii viability on rich medium. Further study revealed that one of these genes codes for a novel bacterial cell division protein.
Identification of genes required for A. baumannii viability
Analysis of strains of A. baumannii, including the model strain 17978 by In-seq technologies have led to the identification of at least 453 genes potentially required for bacterial growth on rich medium (21, 22). Whereas many of these genes are involved in known cellular processes fundamental for cell viability such as DNA replication, transcription and translation, some code for proteins of unknown activity, are thus of great interest as potentially new antimicrobial targets (21). To determine the function of the candidate essential genes annotated as hypotheticals, we first confirmed their essentiality by a conditional gene deletion method. To this end, we employed a set of plasmids that allow inducible gene expression in A. baumannii (24). Each gene of interest was expressed from the arabinose-inducible promoter (23), and the corresponding gene was deleted from the chromosome by the standard allele exchange method (25) (Fig. 1A). Chromosomal deletion mutants harboring the arabinose-inducible gene were tested for essentiality by spotting serially diluted cells onto solid medium with or without arabinose (ara) (Fig. 1A). If the gene of interest is essential, the bacterium should fail to grow on plates without ara.
Our initial analysis of 10 genes (Table S1) identified A1S_0137, A1S_2249, and A1S_3387 (26) that met the criteria of being required for growth on LB agar (Fig. 1B). Importantly, gene A1S_3387 is also essential for A. baumannii growth in the Vogel-Bonner minimal medium with succinate (VBS) as the sole carbon source (27) (Fig. S1). These genes were thus designated as A. baumannii essential genes (Aeg) and Aeg1 (A1S_3387) was chosen for detailed study.
Sequence analysis revealed that Aeg1 has orthologs in a number of Gram-negative bacteria, including Klebsiella pneumoniae, Salmonella enterica members of the Moraxellaceae family and some strains of Escherichia coli, with sequence similarity ranging from 80% to identical (Fig. S2). Aeg1 is predicted to harbor a single transmembrane domain composing of 23 residues close to its amino terminal end (Fig. 1C). To determine the importance of the putative membrane spanning region in its function, we employed a dual inducible system with two compatible plasmids that use promoters that respond to ara and isopropyl-β-D-thiogalactopyranoside (IPTG), respectively. Strain Δaeg1(PBAD::Aeg1, PTAC::Aeg1) was able to grow on medium containing 1% ara or 0.5 mM IPTG (Fig. 1D) indicating that the chromosomal deletion can be complemented by either of the constructs when expression of Aeg1 was induced. Aeg1 is a bitopic integral membrane protein similar to ZipA (28). It has a similar arrangement where the N terminus of the protein traverses the membrane once, while the rest of the protein present in the cytoplasm. Importantly, expression of the Aeg1 mutant lacking the predicted membrane anchoring region (residues 7-29) was unable to restore the growth of the strain (Fig. 1D), suggesting that association with membrane is essential for its activity.
Depletion of Aeg1 caused cell filamentation in A. baumannii
We next examined the morphology of A. baumannii lacking Aeg1 by optical and scanning electron microscopy. Cultures of strain Δaeg1(PBAD::Aeg1) were induced by ara for 14 h, cells diluted into fresh LB with or without ara were allowed to grow for 18 h. We found that cells of cultures grown in medium containing 1% ara exhibited a short rod cell shape similar to that of the wild-type strain (Fig. 2A). In medium without ara most cells of strain Δaeg1(PBAD::Aeg1) displayed an elongated morphology which is in sharp contrast to those of wild-type cells grew under identical conditions (Fig. 2A). Quantitative analysis indicated that in medium supplemented with ara, more than 95% of the cells of strain Δaeg1(PBAD::Aeg1) were shorter than 5 μm. Incubation in medium without ara for 18 h caused more than 96% of the cells to become elongated with cell lengths ranging from 5 μm to over 10 μm (Fig. 2B). The phenotype can be partially reversed after growing the elongated cells in medium containing 1% ara for 4 h (Fig. 2C). These results indicate that depletion of Aeg1 causes cell elongation, a phenotype that had been observed in recent studies using transposon insertion sequencing (20, 29).
Mutations in the cell division gene ftsA bypasses the requirement of Aeg1
To explore the cellular process participated by Aeg1, we sought to isolate suppressor mutants that can survive in standard medium under conditions the gene is not expressed. To this end, cells of strain Δaeg1(PBAD::Aeg1) grown in medium supplemented with ara were exposed to the mutagen ethyl methanesulfonate (EMS), and the treated cells were plated on LB agar without ara. A few colonies were obtained from approximately 6×108 cells and two independent mutants M1 and M2 were chosen for further analysis. In spotting assays using diluted cells, each of these two mutants derived from strain Δaeg1(PBAD::Aeg1) was able to grow on medium without ara at rates comparable to those of wild-type bacteria (Fig. 3A). We then performed whole genome sequencing analysis of these two mutants, which revealed that M1 and M2 had 320 and 328 substitution mutations, respectively. In addition, 659 insertion-deletion (InDel) mutations were found in M1 and 665 such mutations were associated with M2 (Table S2).
Because depletion of Aeg1 caused cell elongation, we first examined mutated genes whose functions are known to be involved in cell division. An E202K mutation caused by a G to A substitution in the first position of the codon was found in the cell division protein FtsA in both M1 and M2 (Table S2). We thus verified the suppression phenotype by introducing a plasmid expressing FtsA or FtsAE202K into strain Δaeg1(PBAD::Aeg1) and examined the growth of the resulting bacterial strains under different induction conditions. Expression of FtsAE202K allowed strain Δaeg1(PBAD::Aeg1) to gain the ability to grow in the absence of ara. In contrast, although the protein was expressed at a comparable level, FtsA cannot suppress the growth defect caused by Aeg1 depletion (Fig. 3B). Consistent with the growth phenotypes, expression of FtsAE202K in strain Δaeg1(PBAD::Aeg1) under conditions in which the complementation gene was not expressed displayed a morphology similar to that of wild-type bacteria (Fig. 3C).
Previous studies have found that several mutations in FtsA bypassed the requirement of a number of essential cell division proteins in E. coli, including ZipA (30), FtsK (31), and FtsN (32). Among these, FtsAR286W (FtsA*) was the first gain-of-function mutation found in E. coli, which allowed the survival of mutants lacking ZipA (ΔzipA) or FtsK (ΔftsK) (30, 31, 33). Additionally, FtsAI143L(34) and FtsAE124A(32) weakly bypassed the requirement of FtsN. These mutations also rescued the FtsLL86F/E87K mutant when overexpressed (35). We thus examined whether similar mutations in FtsA of A. baumannii can bypass the need of Aeg1 by constructing substitution FtsA mutants D124A, V144L and Q285W, which correspond to E124A, I143L, and R286W in its E. coli counterpart, respectively (Fig. S3) and tested them in strain Δaeg1(PBAD::Aeg1). Whereas FtsAQ285W cannot detectably suppress Aeg1 depletion, expression of FtsAD124A or FtsAV144L partially resecured the growth of strain Δaeg1(PBAD::Aeg1) in medium without ara (Fig. 3D). Despite multiple attempts, we were unable to detect the expression of these FtsA mutants by immunoblotting using antibody specific to the Flag epitope fused to these alleles (Fig. 3D, lower panels), suggesting that the low activity exhibited by these mutants may result from low protein levels.
The observation that mutations in genes involved in cell division bypass the need of Aeg1 suggests that this protein participate in the same cellular process. We thus analyzed the cellular localization of Aeg1 in A. baumannii by introducing a plasmid that expresses Aeg1-mCherry from the PBAD promoter. Fluorescence microscopy analysis revealed that Aeg1 localized at the septum of dividing cells (Fig. 3E), which validated the genetic evidence for its role in cell division.
Aeg1 interacts with multiple core components of the A. baumannii divisome
To further explore the possibility that Aeg1 is a component of the A. baumannii divisome, we determined its interactions with known cell division proteins using the Cya-based bacterial two-hybrid method (36). Among the tested proteins FtsZ, FtsA, ZipA, FtsK, FtsQ, FtsL, FtsB, FtsW, FtsI, and FtsN, Aeg1 detectably interacts with ZipA, FtsK, FtsL, FtsB, and FtsN. ZipA and FtsN appearing to have higher binding affinity with Aeg1 as indicated by the hydrolysis of X-gal by derivatives of the E. coli reporter strain BTH101 (36) harboring appropriate protein fusions (Fig. 4A). When the interactions were quantitatively determined by measuring galactosidase activity, the binding between FtsN and Aeg1 appeared the strongest among all examined protein pairs (Fig. 4B).
We next analyzed the localization of Aeg1 and core divisome proteins during cell division of A. baumannii by taking advantage of the elongated morphology of Aeg1-depleted cells, which may allow better resolution of observation via fluorescence microscopy. To this end, we coexpressed the mCherry-Aeg1 fusion with GFP fusion of ZipA, FtsK, FtsL, FtsB, or FtsN in the Δaeg1 mutant from the ara- and IPTG-inducible promoter, respectively. Cultures of strains derived from Δaeg1(PBAD::mCherry-Aeg1) harboring the GFP fusions were induced by ara for 16 h, saturated bacterial cultures were then diluted into fresh LB broth without ara for 6 h to induce the elongation morphology. IPTG (0.25 mM) and ara (0.25%) were added to induce the expression of fusion proteins for 4 h before samples were processed for microscopic analysis. Consistent with results from the protein interaction experiments using the bacterial two-hybrid assay, Aeg1 colocalized with ZipA, FtsK, FtsL, FtsB, and FtsW (Fig. 4C). Thus, Aeg1 interacts with multiple core cell divisome proteins of A. baumannii.
Aeg1 is an essential core cell division protein in A. baumannii
The interaction map between Aeg1 and core divisome proteins prompted us to postulate that the absence of Aeg1 prevented these proteins from localizing to the midcell. To test this hypothesis, we introduced plasmids that direct the expression of GFP fusion of each of the divisome proteins into strain Δaeg1(PBAD::mCherry-Aeg1). We evaluated the protein of Aeg1-mCherry at 2, 4, and 6 h after withdrawing arabinose and found that at the 2 h and 4 h time points mCherry-Aeg1 was still readily detectable (Fig. S4). Importantly, we found that removal of arabinose for 6 h rendered Aeg1-mCherry undetectable in approximately 90% of the cells. We thus used the 6 h inducer depletion to examine the effects of Aeg1 depletion. In cells depleted of Aeg1, all of the examined core division proteins displayed midcell mistargeting, including ZipA, FtsK, FtsB, FtsL, and FtsN (Fig. 5A). These results suggest that Aeg1 is a new member of cell division protein, which plays a role in positioning several other core proteins at the septum of A. baumannii.
During the formation of the divisome, FtsN was believed to be the last protein being recruited to the septum where it converts FtsQLB and FtsA into their active forms, which subsequently activate the septal peptidoglycan synthase FtsWI (34, 37). If the interaction between Aeg1 and FtsN is critical for the ability of the latter to switch on FtsQLB and FtsA, constitutively active mutants of FtsQLB or Fts A should suppress the need of Aeg1 for cell viability. To test this hypothesis, we expressed HA-FtsBE65A and HA-FtsLQ70K of A. baumannii (equivalent to mutants of their E. coli counterparts that allow the bypass of the requirement of FtsN) (Fig. S3) (34, 38) in strain Δaeg1(PBAD::Aeg1) on medium without ara. Although the expression of HA-FtsBE65A was not detectable with the HA-specific antibody, induction of this mutant completely restored the growth of the Δaeg1 mutant in the absence of Aeg1 (Fig. 5B). In contrast, a similarly constructed plasmid that directs the expression of HA-FtsBD68H cannot bypass the requirement of Aeg1 under the same conditions (Fig. 5B). Similarly expressed HA-FtsLQ70K detectably rescued bacterial growth without Aeg1 at a level considerably lower than that of FtsBE65A (Fig. 5C). The low or undetectable activity exhibited by FtsLQ70K and FtsBD68H may result from the low protein levels in strain Δaeg1(PBAD::Aeg1). Nevertheless, these results support our hypothesis that Aeg1 functions upstream of FtsN, probably by functioning as its activator or recruiting factor.
It is known that expression of mutants FtsIK211I, FtsWM269I, and FtsWE289G driven by a strong promoter can bypass the need of FtsN in E. coli (39). The ability of FtsBE65A and FtsAE202K to suppress the growth defect of the Δaeg1 mutant prompted us to examine whether constitutively active alleles of FtsW or FtsI have the same effects. Overexpression of FtsWM254I and FtsWS274G of A. baumannii detectably rescued the growth of the strain in which Aeg1 had been depleted (Fig. 5D). FtsI from A. baumannii does not have a residue corresponding to K211 of its E. coli counterpart (Fig. S3), we thus did not test the effect of this mutation. Therefore, our results demonstrate that Aeg1 may collaborate with FtsN to activate at least a part of FtsQLB and FtsA for subsequently activation of FtsWI during cell division. Together with results that the variant FtsAE202K suppressed the growth defect caused by aeg1 depletion (Fig. 3B), these observations support a model in which the Aeg1-FtsN complex is involved in the activation of FtsQLB and FtsA.
Powerful In-seq DNA sequencing technologies based on saturated transposon insertion libraries have allowed the identification of genes essential for bacterial viability under specific conditions, including commonly used rich media such as LB broth (22). Using a conditional gene deletion method, we have revealed that from 10 putative essential genes identified by this method, three previously annotated as hypotheticals are essential for A. baumannii viability, suggesting that assignment of essentiality to genes identified by methods of this kind need to be individually verified.
Our assignment of aeg1 as an essential gene is consistent with a recent study which revealed its essentiality (termed advA) by transposon insertion sequencing, which also observed cell elongation upon the depletion of this gene (29), Our results provided at least two additional lines of evidence to support the notion that Aeg1 is a core cell division protein in A. baumannii. First, random suppression mutations that bypassed the requirement of Aeg1 mapped to a classic cell division gene and mutations in some cell division genes known to circumvent the need of specific cell division proteins rescued the growth defect caused by Aeg1 depletion. Second, Aeg1 interacts with at least a subset of proteins involved in cell division (Fig. 4A-B).
In the process of bacterial cell division, over 30 distinct proteins are assembled into a complex typically referred to as “divisome” (40). Ten of these are normally essential for both cell division and survival, and they are now mainly suggested to be the core of the divisome in various bacteria (40, 41). These essential division proteins are mostly named Fts (filamentation temperature sensitive), which stems from the fact that inactivating one of these Fts proteins causes cells to become filamentous, which eventually failed to survive (40, 42). Among these, the FtsQLB complex not only serves as a scaffold for recruitment of subsequent divisome proteins (43, 44), but also plays a an important role in regulating the activity of the divisome (34, 37). The constriction control domain (CCD) of FtsL was previously defined as a critical component for switching the conformation of FtsLB from an off to an on state in order to activate FtsWI by enabling the interaction between FtsIperi and the AWI (activation of FtsWI) region of FtsL (34, 37, 45). Division obstruction caused by Aeg1 depletion can be effectively circumvented by suppression mutations (FtsBE65A) in its CCD domain and by activation mutations (M254I and S274G) of FtsW (Fig. 5D), suggesting that Aeg1 functions upstream of these proteins.
In addition to FtsQLB-mediated activation of FtsI in the periplasm, the constitutively active form of FtsA (FtsA*) acting on FtsW in the cytoplasm, these two events functions synergistically to activate FtsWI for sPG synthesis (35). FtsA is a member of the actin family (46, 47), which interacts with FtsN via a unique IC domain positioned between the IA and IIA domains (48–50). Little is known about the function of the IA and IIA domains of FtsA. That the E202K mutation in the IIA domain bypasses the requirement of Aeg1 by A. baumannii (Fig. 3B) suggests that Aeg1 and FtsA work together to activate FtsW.
The maturation of the core divisome is facilitated by the recruitment in a nearly sequential order of the late-division proteins FtsK, FtsQ, FtsL/FtsB, FtsW, FtsI, and FtsN (51–53). Once the latest division protein, FtsN, is recruited, division-specific peptidoglycan synthesis conferred by the FtsWI complex is activated to build the septum (53, 54). More recent studies suggest that by binding to FtsA, a small amount of FtsN is recruited to the division site earlier than previously thought (34, 50, 55) where it induces a conformational switch in both FtsA and the FtsQLB subcomplex on both sides of the membrane (34). Furthermore, binding between FtsA and FtsN is regulated by ZipA (50). Our observation of strong binding of Aeg1 to ZipA and FtsN (Fig. 4A-B) prompted us to propose a model in which Aeg1 participates in cell division by anchoring to the division site together with ZipA where it recruits FtsN, which in turn signals to both the cytoplasmic or periplasmic branches of peptidoglycan synthesis involving FtsA and the FtsQLB complex, respectively (34) (Fig. 6A). The placement of Aeg1 as one of the early arrivals in the hierarchical sequential assembly of the divisome is consistent with the fact that the requirement of Aeg1 can be bypassed by constitutively active mutants of FtsAE202K (Fig. 3), FtsB (FtsBE65A), FtsL (FtsLQ70K) and of FtsW (FtsWM254I and FtsWS274G) (Fig. 5).
It is worth noting that that although homologs of Aeg1 are present in many Gram-negative bacteria, including some recent isolate of E. coli, it appears to be absent in more extensively studied model strains such as K12 (Fig. S2). It is possible that in strain K12, its activity is conferred by a yet unidentified protein that employs a conserved mechanism in cell division. Future study aiming at elucidating the biochemical and structural basis of the activity of Aeg1 and its homologs, and their relationship with other components of the divisome may provide not only insights into the mechanism of bacterial cell division but also leads for the design of antibiotics with novel mechanism of action.
Materials and methods
Bacterial strains, growth conditions, and plasmids construction
All bacterial strains and plasmids used in this study are listed in Table S3. All A. baumannii strains were derivatives of strain ATCC17978 (56). Bacteria were grown in Luria Bertani (LB) broth or agar. Antibiotics were added to media when necessary. For E. coli, the concentrations used were: ampicillin, 100 µg/mL, kanamycin, 30 μg/mL, chloramphenicol, 30 µg/mL, and gentamicin, 10 μg/mL. For A. baumannii, kanamycin, 30 μg/mL, gentamicin, 10 μg/mL, and streptomycin, 100 μg/mL.
To construct pUT18C-Flag, two oligos with sequences coding for the Flag tag and appropriate restriction sites were mixed at a 1: 1 ratio, heated to 100°C for 5 min and allowed the mixed DNA to anneal by gradually cooling down to room temperature. The resultant DNA fragment was then inserted into SalI/ClaI digested pUT18C (36). We introduced an EcoRV site into the inserted DNA fragment to facilitate the identification of plasmids carrying the intended insert. To make pJL05 that allows PTAC-driven expression of HA-tagged protein, primers containing the coding sequence of the HA tag were used to amplified the promoter region of pJL02 (24), PCR product was inserted as a PciI/BamHI fragment. This manipulation replaced the Flag tag on pJL02 with an HA tag. pJL03 is a pVRL1-based plasmid harboring PBAD, which allows expressing Flag-tagged proteins in A. baumannii (24).
The DNA fragment were amplified from genomic DNA of A. baumannii strain and digested with appropriate restriction enzyme (Table S3), following insert into similarly digested plasmids. The experimental procedure entails fusion of the GFP gene with the target gene, followed by ligation of the resulting construct into the pJL05 vector for subsequent expression.
Introduction of plasmids into A. baumannii
Plasmids were introduced into A. baumannii by electroporation (57) or by triparental mating. Electrocompetent cells were prepared using a previously described protocol (58) with minor modifications. Briefly, saturated cultures of A. baumannii were diluted 1:100 into 50 mL LB medium to grow for 18 h at 37°C and cells were harvested by centrifugation (4000g) at room temperature (RT) for 5 min. Cells were washed twice with 10 mL of 10% glycerol, resuspended in 1 mL of 10% glycerol, cells aliquoted in 50 μL. were stored at −80°C. For transformation, 1 μg of plasmid DNA was mixed with 50 μL competent cells and electroporation was performed in 0.2-cm cuvettes on a Bio-Rad Gene Pulser system (2.5 kV/cm, 25 μF, 200 Ω).
The E. coli MT607 harboring pRK600 (59) was used for triparental mating. Briefly, 100 μL of overnight cultures of the donor, recipient, and the helper strain were mixed and cells were harvest by centrifugation (4000g) for 5 min. Mixed cells washed twice with 1 mL of sterile water were spotted onto LB agar and the mating was allowed to proceeded for 4-6 h at 37°C before plating onto selective medium.
Construction of in-frame deletion mutants of A. baumannii
To delete the putative essential genes, we first introduced a plasmid expressing the gene of interest into A. baumannii and the resulting strains were used to construct chromosomal deletion mutants using a method based on the suicide plasmid pSR47s (25). Briefly, the coding region of the gene was inserted into pJL03, which allows expression of Flag-tagged protein from the PBAD promoter (24) and the resulting plasmid was introduced into A. baumannii by electroporation.
To construct the plasmid used for in-frame deletion of A. baumannii genes, 1.0 kilobase DNA fragment was amplified from the upstream and downstream of the gene to delete, respectively, digested with appropriate restriction enzymes and inserted into similarly digested pSR47s (25). The primers were designed so that in each case the gene to be deleted was replaced with an open reading frame consisting of the first and last 15 residues linked by two amino acids encoded by the sequence of the restriction enzyme used to fuse the two DNA fragments (Table S3). The plasmid was then introduced into the A. baumannii strain harboring the pJL03 derivative expressing the gene to be deleted from the PBAD promoter by triparental mating. Transconjugants obtained on LB agar containing 1% ara were grown in LB broth containing 5% sucrose for 18 h prior to being streaked onto LB agar supplemented with 5% sucrose. Deletion mutants were identified by colony PCR with primers corresponding to the 5’ end of the upstream fragment and the 3’ of the downstream fragments.
Ethyl methanesulfonate (EMS) mutagenesis
The A. baumannii strain Δaeg1(PBAD::Aeg1) was grown in LB broth containing 1% ara to saturation, cells from 2-mL culture collected by centrifugation were washed twice with cold buffer A [60 mM K2HPO4, 33 mM KH2PO4, 7.6 mM (NH4)2SO4, 1.7 mM sodium citrate, PH=7]. Cells resuspended in 2x buffer A supplemented with 1.4% EMS were incubated in a shaker (220 rpm) at 37°C for 15 min for 30 min, 45 min and 60 min, respectively. Treated cells harvested by centrifugation were washed twice with buffer A prior to being plated onto LB agar supplemented with 5% glucose. Colonies appeared were purified and tested by spotting diluted cells onto LB agar with or without ara, respectively to confirm the ability to grown in the absence Aeg1. To eliminate the possibility that the ara-independent growth phenotype was due to mutations in the PBAD promoter, the plasmid from each mutant candidate was rescued and the promoter region was sequenced. Only mutants harboring an intact PBAD promoter were retained for further study.
Genome sequencing was performed by Suzhou Golden Wisdom Biological Technology Co., Ltd, and mutations were identified using the genome of the parent strain ATCC 17978 as reference (26).
Bacterial Two-Hybrid assays
To assess interactions between Aeg1 and A. baumannii Fts proteins, we employed the Bordetella pertussis adenylate cyclase (Cya)-based bacterial two-hybrid system (36). The coding region of Aeg1 was fused to the T25 fragment of Cya and testing Fts proteins were individually fused to the T18 fragment on pUT18C-Flag, which allows the detection of the fusion protein with the Flag-specific antibody (Table S3). Interaction was evaluated in the cya- E. coli strain BTH101 (36) by the ability of the testing strains to hydrolyze X-Gal. Quantitative measurement of β-galactosidase activity with performed with ortho-nitrophenyl-β-galactoside (ONPG) using an established protocol (60). Testing bacterial strains were cultured in 2 mL LB broth containing 0.5 mM IPTG for 18 h, and 0.2 mL cells were harvest and resuspended in 1 mL Z-buffer (60 mM Na2HPO4, 40 mM NaH2PO4·H2O, 1 mM MgSO4, 10 mM KCl, and 7 mL of 2-mercaptoethanol per liter). After adding 50 µL 1% (m/v) SDS and 100 μL of chloroform, cells were lysed by 30 s vortex and 50 μL of ONPG (40 mg/mL) was added. The reaction was terminated with 500 µL of 1 M Na2CO3 after ONPG hydrolysis had occurred as determined the presence of the yellow color product. β-galactosidase activity is defined as in Miller units using the following equation: (OD420×1000)/ [(time of incubation in min)x(volume of cell suspension in mL)x(cell density of the culture in OD600)]. All assays were done in triplicate.
Antibodies and immunoblotting
HA-specific antibody (1:1000) was purchased from Santa Cruz (Cat #sc-7392), and rabbit anti-ICDH (1:2000) was described previously (24). To prepare protein samples, we diluted (1:100) saturated bacterial cultures into fresh LB broth. When OD600 of the cultures reached 0.6, IPTG was added to a final concentration of 0.5 mM. Approximately 1×109 cells were collected, resuspended in 100 μL SDS sample buffer and were boiled for 5 min. Proteins separated by SDS-PAGE were transferred onto nitrocellulose membranes, which were first blocked with 5% nonfat milk for 1 h followed by incubation with appropriate primary antibodies for 14 h. Washed membranes were incubated with the appropriate IRDye-labeled secondary antibodies and signals were detected using an Odyssey CLx system (LI-COR).
To determine the cellular localization of Aeg1 and its colocalization with members of the divisome, we constructed pJL03::Aeg1-mCherry, which expressed the fusion from the PBAD promoter (23). GFP chimera of Fts proteins were expressed from pJL05 driven by the PTAC promoter inducible by IPTG. Each of the GFP-Fts fusion was introduced into strain Δaeg1(PBAD::mCherry-Aeg1). All strains were maintained in media containing 1% ara to ensure their viability.
To visualize the localization of the fusions, bacterial strains were grown to saturation in LB broth containing ara and the cells washed twice with PBS were diluted to a density of approximately 5×106 cells/mL. The cultures were split into two 2-mL subcultures, the one used to determine how the absence of Aeg1 impacted the cellular localization of Fts proteins received only IPTG but not ara. Cultures were incubated in a shaker for an additional 6 h, and 100 μL cells were withdrawn and fixed by incubation in glutaraldehyde for 20 min at RT. After washing twice with PBS, samples were stained with Hoechst 33342 (1:5000) (Invitrogen, cat#H3570) and mounted on slides for observation with an Olympus IX-83 fluorescence microscope.
Aeg1 homologs were identified using tBLASTn (61) using the Aeg1 protein (A1S_3387) from strain ATCC17978 (GenBank: CP000521.1) as the query sequence. Clustal omega (https://www.ebi.ac.uk/Tools/msa/clustalo/) was used to perform multiple sequence alignments. The homology comparison of Fts proteins between E. coli and A. baumannii was also performed using Clustal omega. Jalview was used for editing and viewing sequence alignments (62). The transmembrane regions of Aeg1 were predicted by the SMART Web site (63).
Data quantitation, statistical analyses
Student’s t-test was used to compare the mean between two groups each with at least three independent samples.
This work in part was supported by the Natural Science Foundation of Jilin Province grant YDZJ202201ZYTS025 (XC) and Jilin Science and Technology Agency grant 20200403117SF (LS).
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